Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins

Abstract

et al.. Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins. Biosensors and Bioelectronics, Elsevier, 2019, 146, pp. AbstractKnowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion of three pathogens, Aeromonas salmonicida, Aeromonas hydrophila and Vibrio harveyi, to Atlantic salmon mucins isolated from different epithelial sites, using SPR and microtiter-based binding assays. We demonstrated that performing bacterial binding assays to mucins using SPR is feasible and has advantages over microtiter-based binding assays, especially under flow conditions. The fluid flow in the SPR is linear and continuous and SPR enables real-time reading of mucin-bacterial bonds, which provides an in vivo-like setup for analysis of bacterial binding to mucins. The variation between technical replicates was smaller using SPR detection compared to the adenosine 5'-triphosphate (ATP) bioluminescence assay in microtiter plates. Furthermore, we demonstrated that the effect of flow on pathogen-mucin interaction is significant and that bacterial adhesion differ non-linearly with flow rates and depend on the epithelial source of the mucin.

Unraveling the liquid gliding on vibrating solid liquid interfaces with dynamic nanoslip enactment

“…In contrast, our work shows changes in frequency of the quartz crystal microbalance simply due to change in flow rate of the buffer which is associated with liquid slip and not exclusively faster biomolecular binding. Similar concepts can also be extended to isolate slip effects in other flow based sensing systems such as surface plasmon resonance systems or routine electrochemical sensing platforms integrated with automatic fluidic systems where effects of flows may as well be associated with sensor sensitivity and binding affinities [37][38][39] . In addition, the equations developed also serve as a fundamental basis for the detection of liquid slip length using vibrating solid based sensors.…”

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

“…Batch configuration is less used because it requires longer time frames for signal stabilization. However, miniaturized instruments such as fiber optic surface plasmon resonance sensors (FO-SPR, where the prism is replaced by the core of an optical fiber) are better suited for monitoring interactions between immobilized ligands and different bacterial strains, similar to in vivo conditions [ 17 ]. On the other hand, flow conditions are mostly used for real-time monitoring and the determination of kinetic parameters.…”

Glycocalyx Components Detune the Cellular Uptake of Gold Nanoparticles in a Size- and Charge-Dependent Manner

“…There are a lot of methods to investigate the cellular uptake of NPs by cells. For visualization, the following techniques can be applied, such as the confocal, fluorescence, dark-field, differential interference contrast microscopies, surface-enhanced Raman spectroscopy, and transmission electron microscopy (TEM). − Inductively coupled plasma mass spectrometry (ICP–MS), ICP atomic emission spectroscopy, electron spin resonance, and fluorometry are demonstrated to measure the number of delivered NPs inside the cells. − ,− However, the most important drawback of these methods is that they provide end-point data, thus do not give information on the dynamics and kinetics of the uptake process. , Nowadays, label-free biosensors and techniques receive more focus − even in monitoring the real-time cellular uptake of NPs. ,, …”