Mucilaginibacter inviolabilis sp. nov., isolated from the phycosphere of Haematococcus lacustris NIES 144 culture

Abstract

A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterial strain, designated HC2T, was isolated from the phycosphere of Haematococcus lacustris NIES 144 culture. Strain HC2T was able to grow at pH 4.5–8.0, at 4–32 °C and in the presence of 0–2 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain HC2T was affiliated to the genus Mucilaginibacter and shared the highest sequence similarity with Mucilaginibacter lappiensis ANJKI2T (98.20 %) and Mucilaginibacter sabulilitoris SMS-12T (98.06 %). Strain HC2T contained summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C15 : 0 as the major fatty acids (>10.0 %). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. The respiratory quinone was menaquinone 7 (MK-7). The genomic DNA G+C content was 42.0 %. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain HC2T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter inviolabilis sp. nov. is proposed. The type strain is HC2T (=KCTC 82084T=JCM 34116T).

Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

“…Growth was observed in R2A broth (Difco) at different pH values (4.0–12.0 at 0.5 pH unit intervals) and temperatures (4, 10, 15, 20, 25, 30, 37, and 40°C) for five days [ 13 ]. Catalase and oxidase activity were detected as described previously [ 2 ]. Metabolic activity was determined by commercial API kits (bioMérieux, France).…”

“…The ability of strain JC4 T to grow on various media, including tryptone soy agar (TSA, Difco), nutrient agar (NA, Difco), and Luria-Bertani agar (LBA, Difco) was recorded for up to five days of incubation at 25 o C. Growth was observed in R2A broth (Difco) at different pH values (4.0-12.0 at 0.5 pH unit intervals) and temperatures (4,10,15,20,25,30,37, and 40 o C) for five days [13]. Catalase and oxidase activity were detected as described previously [2]. Metabolic activity was determined by commercial API kits (bioMérieux, France).…”

“…Phylogenetically, this genus is affiliated with the family Sphingobacteriaceae in the class Sphingobacteriia [ 1 ]. Currently, at least 74 validly published species have been isolated from diverse ecological niches, such as phycosphere of microalgae Haematococcus [ 2 ], fresh water [ 3 ], marine sand [ 4 ], sediment [ 5 ], and soil [ 6 ]. Members of the genus Mucilaginibacter were identified as Gram-negative, non-motile, and rod-shaped [ 1 ].…”

Mucilaginibacter straminoryzae sp. nov., isolated from rice straw used for growing periphyton

“…The pan-genome analysis of Mucilaginibacter species revealed they have a complex carbohydrate utilization process [12]. Members of this genus have been reported to produce exopolysaccharides [13], to degrade cellulose, hemicellulose [14] and chitin [5], and to promote the growth of plant and microalgae [15, 16]. Additionally, some Mucilaginibacter species can produce carotenoids, suggesting their potential application in the food, beverage, and cosmetics industries [5].…”

Mucilaginibacter rivuli sp. nov., isolated from a water rivulet

“…The major cellular fatty acids are iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (C 16 : 1 ω7c and/or C 16 : 1 ω6c) [3][4][5][6][7][8][9][10]. Some species are characterized by producing exopolysaccharides and degrading cellulose, hemicellulose, pectin, xylan and laminarin, and have potential plant-growth-promoting abilities [13,14]. In this study, strain HMF5004 T is described as a representative of a novel species of the genus Mucilaginibacter based on the results of a polyphasic approach.…”