Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

Abstract

Phylogenetic AnalysisGenomic DNA was extracted from three-day-old cultures according to Ko et al. [16]. Amplification and sequencing of the 16S rRNA gene were performed using universal primer set 27F (5'-AGAGTTTGATCATGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') [17]. The nearly full-length 16S rRNA gene sequence of strain JC4 T (1,402 bp) was compared with those of valid species in the EzTaxon server. Phylogenetic trees were A Gram-stain-negative, rod-shaped bacterial strain, JC4 T , was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4 T is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133 T . The average nucleotide identity and digital DNA-DNA hybridization values between strain JC4 T and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4 T contained summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) and iso-C 15:0 as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4 T was determined to be 42.44%. The above polyphasic evidences support that strain JC4 T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4 T (= KCTC 92230 T = LMG 32715 T ).