MspI Allelic Pattern of Bovine Growth Hormone Gene in Indian Zebu Cattle (Bos indicus) Breeds

Sodhi, Monika; Mukesh, Manishi; Prakash, B.; Mishra, Bina; Sobti, Ranbir Chander; Singh, Kiran; Singh, Satbir; Ahlawat, Sonika

doi:10.1007/s10528-006-9068-4

Cited by 38 publications

(41 citation statements)

References 11 publications

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“…The development in molecular genetic techniques has made it possible to identify differences between individuals at the DNA level. Recently, genetic polymorphisms at candidate genes affecting economic traits have stimulated substantial research interest because of their impending utilization as an aid to genetic selection and to demarcate evolutionary relationships in different livestock breeds (Sodhi et al, 2007). Association of several polymorphic sites (SNPs) in different candidate genes with economic traits has been much investigated in different animal species.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of six Sudanese camel breeds

Ishag¹,

Reißmann²,

Peters³

et al. 2011

SA J. An. Sci.

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The objective of this study was to sequence the growth hormone (GH) gene in Sudanese camel breeds (Kenani, Lahwee, Rashaidi, Anafi, Bishari and Kabbashi) searching for single nucleotide polymorphisms (SNPs) and contribute to the phenotypic characterization of the multitude of camel ecotypes in Sudan. This will also afford the chance of investigating the possibility of the presence of correlations between body measurements and SNPs of GH gene. A length of 1732 bp, spanning the region between -44 bp upstream of the first exon and +37 bp downstream of the last exon was sequenced in two animals from each breed. The sequence comparison of Sudanese camel GH sequences with the GenBank sequence identified one single nucleotide polymorphism (SNP). The SNP was detected in the non coding region (intron 1) in position AJ575419:g.419C>T. A PCR-RFLP method was used to genotype 181 animals representing the six tested Sudanese breeds for detected SNP. The Bishari and Anafi breeds that are classified as riding camels had slightly higher T allele frequencies (0.57 and 0.48, respectively) than those of the other four breeds which are classified as pack camels. The effect of genotype with regard to the SNP g.419C>T on those traits was not significant. ________________________________________________________________________________

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Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of six Sudanese camel breeds

Ishag¹,

Reißmann²,

Peters³

et al. 2011

SA J. An. Sci.

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“…Recently, genetic polymorphisms at candidate genes affecting economic traits have stimulated substantial research interest because of their impending utilization as an aid to genetic selection and to demarcate evolutionary relationships in different livestock breeds (Sodhi et al, 2007 Gupta et al, 2007).…”

mentioning

confidence: 99%

Genetic Variations in Two Casein Genes Among Maghrabi Camels Reared in Egypt

Othman¹,

Nowier²,

El-Denary³

2016

Biosci., Biotech. Res. Asia

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Camels play an important socio-economic role within the pastoral and agricultural system in the dry and semidry zones of Asia and African where they are dual purpose animals (meat and milk). In spite of the effective role of casein genes with their polymorphisms on quantitative traits and technological properties of milk, the studies on genetic polymorphism of camel milk genes are limited. This work aimed to identify the genetic polymorphisms and SNPs of two casein genes in Maghrabi camel breed in Egypt. The amplified fragments at 488-bp of κ κ κ κ κ-CN gene were digested with AluI endonuclease. The results showed the presence of three genotypes; CC (12%), TT (48%) CT (40%). The sequence analysis of two detected alleles declared the presence of a SNP (C→ → → → →T) at position 121 in amplified fragments. The nucleotide sequences of κ κ κ κ κ-CN alleles C and T were submitted to GenBank with accession numbers; KU055605 and KU055606, respectively. The primers used in this study amplified 942-bp fragments of ás1-casein gene. The results of SmlI digestion did not showed any restriction site whereas the digestion with AluI endonuclease revealed the presence of two restriction sites AG^CT at positions 68^69 and 631^632 in amplified fragments. The nucleotide sequence of monomorphic α α α α αs1-casein gene was submitted to GenBank with accession number KU145820.In conclusion, the genetic characterization of genes associated with milk yield and composition in camel is considered an essential step towards its genetic improvement through the selection of superior animals depending on the favorable alleles and genotypes; marker assisted selection (MAS).

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“…Variants of alleles in GH locus did not change genetic equilibrium in that population (Table 2). Previous study, Sodhi et al (2007) reported that frequency of MspI-in Indian cattle breeds was higher than MspI+ in taurine breeds (Bos taurus) from northern Eroupe, Mediteranean countries, and America. Frequencies of MspI-alleles were 0.94; 0.90; 0.88; 0.90 and 0.80 for Sahiwal, Tharparkar, Gir, Ongole and Hariana, respectively.…”

Section: Mspi(+/+) Mspi(+/-) and Mspi(-/-mentioning

confidence: 90%

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

Hartatik

Putra

Volkandari

et al. 2018

J. Indonesian Trop. Anim. Agric.

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ABSTRAKGen Growth Hormone (GH) merupakan sebuah gen yang mengontrol sintesis hormon pertumbuhan dan berperan dalam metabolisme tubuh. Identifikasi polimorfisme suatu gen penting dilakukan untuk memperoleh informasi awal dalam mengetahui penanda genetik yang berhubungan dengan sifat-sifat ekonomis yang diinginkan. Pemetaan genetik sangat penting dalam proses seleksi. Sapi Pesisir adalah salah satu sapi lokal Indonesia yang ditemukan di Sumatera Barat dan sangat adatif terhadap lingkungan setempat. Penelitian ini bertujuan untuk mengidentifikasi genotip berdasarkan sebagian gen Growth Hormone (GH891│MspI) pada sapi Pesisir dan sapi Persilangan Simmental-Pesisir (SimPes) dan hubungannya dengan sifat pertumbuhan (ukuran tubuh dan berat badan). Sebanyak 30 sampel darah sapi yang terdiri dari 15 Sapi Pesisir dan 15 Sapi SimPes digunakan dalam penelitian ini. Ukuran tubuh dan berat badan sapi diukur pada umur 12-18 bulan. Metode analisis yang digunakan adalah Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Hasil menunjukkan bahwa terdapat tiga (3) variasi genotip (MspI-/-, MspI+/-, MspI+/+) dengan frekuensi alel sebesar 53,3% untuk alel MspI-dan 46,7% untuk MspI+. Tidak ditemukan hubungan yang signifikan antara ukuran tubuh dan bobot badan kedua bangsa sapi tersebut berdasarkan ketiga genotip gen GH (GH891│MspI). Kesimpulan, terdapat tiga genotip gen GH pada populasi sapi Pesisir dan SimPes dan tidak berpengaruh signifikan terhadap performans pertumbuhan sapi.Kata Kunci: genotip, enzim MspI, gen Pertumbuhan, sifat pertumbuhan, sapi Pesisir, sapi SimPes ABSTRACTGrowth hormone gene that controls metabolism and body size of animals and polymorphism of the gene is known related to performance of livestock. Identification of gene polymorphisms was important to get early information to determine genetic markers associated with economically desirable traits. Genetic mapping is one of important characterizations in the selection process. Pesisir cattle is one of local cattle in Indonesia that are reared in West Sumatera and have become adaptive to local environment. The present study aimed to identify the genotype of Growth Hormone gene (GH891│MspI) of the Pesisir cattle and crossbred Simmental-Pesisir cattle (designated as SimPes) and relationship between growth performance (body weight and body size). The present study was Pesisir and Crossed SimPes Cattle (T. Hartatik et al.) 1 conducted to 30 blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight and body size were measured at 12-18 months of age. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in this research. The results showed that there exist three variant genotypes (MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI-and MspI+ were 53.3% and 46.7%, respectively. Non-significant effect has found between GH's genotype and growth traits (Body measurement and body weight) in both of breed cattle. In conclusion, three variant genotypes (GH891│MspI) in Pes...

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MspI Allelic Pattern of Bovine Growth Hormone Gene in Indian Zebu Cattle (Bos indicus) Breeds

Cited by 38 publications

References 11 publications

Phenotypic and molecular characterization of six Sudanese camel breeds

Phenotypic and molecular characterization of six Sudanese camel breeds

Genetic Variations in Two Casein Genes Among Maghrabi Camels Reared in Egypt

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

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