Phenotypic and molecular characterization of six Sudanese camel breeds

Ishag, Ibrahim A.; Reißmann, Monika; Peters, Kurtis; Musa, Lutfi M-A; Ahmed, Mohamed S.

doi:10.4314/sajas.v40i4.65244

Cited by 16 publications

(24 citation statements)

References 11 publications

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“…Many candidate genes have been identified and selected for analysis based on a known relationship with productivity traits (Spelman and Bovenhius, 1998). In farm animals, promising candidate genes for many traits are in the growth hormone axis (Ishag et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Othman¹,

Alam²,

El-Kader³

et al. 2015

Biotechnology

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A B S T R A C T Small ruminants are considered as one of the major sources of meat and milk production in Egypt. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. In this study, we aimed to detect the genetic polymorphism of GH gene in major Egyptian sheep and goat breeds using PCR-RFLP and identify the single nucleotide polymorphism between different genotypes detected in these breeds. The primers used in this study flanked a 422 bp fragment from exons 2 and 3 of GH gene in sheep and goat. These PCR amplified fragments were digested with HaeIII endonuclease and the results showed the presence of two genotypes; GG and AG with the absence of AA genotype in 149 tested animals for this gene. The total frequencies were 43.56 and 56.44% for GG and AG genotypes, respectively in 101 tested sheep animals whereas in goat animals, the total frequencies were 12.5 and 87.5% for GG and AG genotypes, respectively in 48 tested goat animals. The sequence analysis of purified PCR products representing these two detected genotypes declared the presence of a SNP (G6A) at position 55 in the amplified fragment which is responsible for the destruction of the restriction site GG^CC and consequently the presence of two different alleles G and A which were named in this work according to the detected SNP. The nucleotide sequences of sheep GH alleles G and A as well as goat GH alleles G and A were submitted to nucleotide sequences database NCBI/Bankit/GenBank and have accession numbers: KP120857, KP120858, KP120859 and KP120860, respectively. In conclusion, production improvements can be achieved by using new genetic technology for better selection of heritable traits through marker-assisted selection. Due to the reported association between genotype possess A/G nucleotide with different growth trait parameters, we recommend to increase this heterozygous genotype in native small ruminant breeds through the selection of animals have AG genotype of GH gene and enter them in breeding programs of Egyptian small ruminants as a way to increase their production traits.

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Section: Introductionmentioning

confidence: 99%

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Othman¹,

Alam²,

El-Kader³

et al. 2015

Biotechnology

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“…They have been domesticated about 3000 years ago and are present in high numbers in the arid parts of Africa, particularly in the arid lowlands of Eastern Africa (Somalia, Sudan, Ethiopia, Kenya and Djibouti) (Schwartz and Dioli, 1992). The dromedary camel contributes significantly to family food security in semi dry and dry climates, and is a major component of the agropastoral systems in vast pastoral areas in Asia and Africa (Ishag et al, 2010). Camels are economically important animals in Egypt where they are dual purpose animals (meat and milk).…”

Section: Resultsmentioning

confidence: 99%

“…The PCR products were electrophoresed on 2% agarose gel and stained with ethidium bromide to check amplification product. The primers used in this study were designed on the basis of DNA sequence of the camel GH gene (Ishag et al, 2010). The primer sequences are: F:…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Detection of MspI polymorphism and the single nucleotide polymorphism (SNP) of GH gene in camel breeds reared in Egypt

El-Aziem¹,

El-Kader²,

Alam³

et al. 2015

Afr. J. Biotechnol.

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Growth hormone (GH) is an anabolic hormone synthesized and secreted by the somatotroph cells of the anterior lobe of the pituitary gland in a circadian and pulsatile manner, the pattern of which plays an important role in postnatal longitudinal growth and development, tissue growth, lactation, reproduction as well as protein, lipid and carbohydrate metabolism. The aim of this study was to detect the genetic polymorphism of GH gene in five camel breeds reared in Egypt which are Sudany, Somali, Mowaled, Maghrabi and Falahy, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Also, this work aimed to identify the single nucleotide polymorphism (SNP) between different genotypes detected in these camel breeds. The amplified fragment of camel GH at 613-bp was digested with the restriction enzyme MspI and the result reveals the presence of three different genotypes; CC, CT and TT in tested breeds. Significant differences were recorded in the genotype frequencies between these camel breeds. The result shows that the Maghrabi breed that is classified as a dual purpose camels had higher frequency for allele C (0.75) than those in the other tested four breeds. The sequence analysis declared the presence of a SNP (C264T) in the amplified fragment which is responsible for the elimination of the restriction site C^CGG and consequently the appearance of two different alleles C and T. The nucleotide sequences of camel GH alleles T and C were submitted to nucleotide sequences database NCBI/Bankit/GenBank and have accession numbers: KP143517 and KP143518, respectively. It is concluded that only one SNP C→T was detected in GH gene among the five tested camel breeds reared in Egypt and this nucleotide substitution can be used as a marker for the genetic biodiversity between these camel breeds. Also, due to the possible association between allele C and higher growth rate, we can used it in marker assisted selection (MAS) for camels in breeding program as a way for enhancement of growth trait in camel breeds reared in Egypt.

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“…Growth hormone has a diverse biological activities including somatogenic, lactogenic (Ishag et al, 2010), it plays a key role in increasing growth performance or milk yield (Etherton and Bauman, 1998). In ruminants, GH is known to be responsible for galactopoesis and for persistency of lactation (Svennersten-Sjaunja and Olsson, 2005), and results in more efficient milk production in dairy cows.…”

Section: Introductionmentioning

confidence: 99%

Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep

Abdelmoneim

Brooks

Afifi

et al. 2016

IJAR

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This study aimed to evaluate the relationship between growth hormone (GH) gene polymorphism and estimated body weight in Harri sheep. One hundred Harri sheep lambs were used to determine the birth weight (BW) and weight at 120 days of age. The daily live-weight gain (DLWG) 0-120 days (g) was calculated. The complete CDS of the Harri sheep GH gene is 2117pb in length (GenBank acc. no. KU255857). Three novel SNPs were detected by comparing with GenBanke acc. no. X12546_1. The G871A SNP in intron II, G1383A in exon IV that resulted in conversion of the amino acid arginine number 121 to lysine (R121K) and the A1509G in intron IV. Each SNP was found on both alleles the mutant homozygote was more common (48, 56 and 50%) than the heterozygote (30, 18 and 20%) for G871A, G1383A and A1509G respectively. A positive significant (P<0.05) correlation between growth traits (BW, 120 day body weight and DLWG) and SNP and a highly significant correlation with the genotype were detected. The regression analysis indicated the positive effect of genotype and SNPs on the growth traits. Individuals carrying homozygote mutant alleles had the heaviest body weight and the highest DLWG. Consequently, these SNP may be useful indicators in the selection of lambs for higher growth rate and meat production.

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Phenotypic and molecular characterization of six Sudanese camel breeds

Cited by 16 publications

References 11 publications

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Detection of MspI polymorphism and the single nucleotide polymorphism (SNP) of GH gene in camel breeds reared in Egypt

Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep

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