Moving Domain Computational Fluid Dynamics to Interface with an Embryonic Model of Cardiac Morphogenesis

Lee, Juhyun; Esmaily, Mahdi; Kung, Ethan; Cao, Hung; Beebe, Tyler; Miller, Yury I.; Roman, Beth L.; Lien, Ching‐Ling; Neil, C.; Marsden, Alison L.; Hsiai, Tzung K.

doi:10.1371/journal.pone.0072924

Cited by 54 publications

(66 citation statements)

References 49 publications

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“…Cardiac contraction exposes endocardial cells to mechanical forces across many different length and time scales (Bartman et al, 2004;Boselli et al, 2015;Lee et al, 2013;Vermot et al, 2009). Endocardial cells are particularly well positioned to detect hemodynamic forces, including shear stress (the frictional force of parallel flow).…”

Section: Primary Cilia Are Required For Notch Activation In Endocardimentioning

confidence: 99%

Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish

et al. 2015

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Congenital heart disease often features structural abnormalities that emerge during development. Accumulating evidence indicates a crucial role for cardiac contraction and the resulting fluid forces in shaping the heart, yet the molecular basis of this function is largely unknown. Using the zebrafish as a model of early heart development, we investigated the role of cardiac contraction in chamber maturation, focusing on the formation of muscular protrusions called trabeculae. By genetic and pharmacological ablation of cardiac contraction, we showed that cardiac contraction is required for trabeculation through its role in regulating notch1b transcription in the ventricular endocardium. We also showed that Notch1 activation induces expression of ephrin b2a (efnb2a) and neuregulin 1 (nrg1) in the endocardium to promote trabeculation and that forced Notch activation in the absence of cardiac contraction rescues efnb2a and nrg1 expression. Using in vitro and in vivo systems, we showed that primary cilia are important mediators of fluid flow to stimulate Notch expression. Together, our findings describe an essential role for cardiac contraction-responsive transcriptional changes in endocardial cells to regulate cardiac chamber maturation.

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Section: Primary Cilia Are Required For Notch Activation In Endocardimentioning

confidence: 99%

Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish

et al. 2015

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“…Some models have even been made of fish hearts, e.g. a fluid flow model of the larval zebrafish heart [35] and a mechanical model of the carp heart [36], but no electrical simulations exist for the zebrafish larva.…”

Section: Introductionmentioning

confidence: 99%

3D Finite Element Electrical Model of Larval Zebrafish ECG Signals

Crowcombe¹,

Dhillon²,

Hurst³

et al. 2016

PLoS ONE

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Assessment of heart function in zebrafish larvae using electrocardiography (ECG) is a potentially useful tool in developing cardiac treatments and the assessment of drug therapies. In order to better understand how a measured ECG waveform is related to the structure of the heart, its position within the larva and the position of the electrodes, a 3D model of a 3 days post fertilisation (dpf) larval zebrafish was developed to simulate cardiac electrical activity and investigate the voltage distribution throughout the body. The geometry consisted of two main components; the zebrafish body was modelled as a homogeneous volume, while the heart was split into five distinct regions (sinoatrial region, atrial wall, atrioventricular band, ventricular wall and heart chambers). Similarly, the electrical model consisted of two parts with the body described by Laplace’s equation and the heart using a bidomain ionic model based upon the Fitzhugh-Nagumo equations. Each region of the heart was differentiated by action potential (AP) parameters and activation wave conduction velocities, which were fitted and scaled based on previously published experimental results. ECG measurements in vivo at different electrode recording positions were then compared to the model results. The model was able to simulate action potentials, wave propagation and all the major features (P wave, R wave, T wave) of the ECG, as well as polarity of the peaks observed at each position. This model was based upon our current understanding of the structure of the normal zebrafish larval heart. Further development would enable us to incorporate features associated with the diseased heart and hence assist in the interpretation of larval zebrafish ECGs in these conditions.

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“…5(d), respectively. The strain rate of the beating heart could also be computed based on the girth variation of the outer segmented boundary within a cardiac cycle [32] [ Fig. 5(e)].…”

Section: Image-based Quantification Of Beating Embryonic Zebrafish Heartmentioning

confidence: 99%

Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope

Guan¹,

Lee²,

Jiang³

et al. 2015

Biomed. Opt. Express

Self Cite

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Abstract:We developed a compact plane illumination plugin (PIP) device which enabled plane illumination and light sheet fluorescence imaging on a conventional inverted microscope. The PIP device allowed the integration of microscope with tunable laser sheet profile, fast image acquisition, and 3-D scanning. The device is both compact, measuring approximately 15 by 5 by 5 cm, and cost-effective, since we employed consumer electronics and an inexpensive device molding method. We demonstrated that PIP provided significant contrast and resolution enhancement to conventional microscopy through imaging different multi-cellular fluorescent structures, including 3-D branched cells in vitro and live zebrafish embryos. Imaging with the integration of PIP greatly reduced out-of-focus contamination and generated sharper contrast in acquired 2-D plane images when compared with the stand-alone inverted microscope. As a result, the dynamic fluid domain of the beating zebrafish heart was clearly segmented and the functional monitoring of the heart was achieved. Furthermore, the enhanced axial resolution established by thin plane illumination of PIP enabled the 3-D reconstruction of the branched cellular structures, which leads to the improvement on the functionality of the wide field microscopy. 1938-1940 (2007

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Moving Domain Computational Fluid Dynamics to Interface with an Embryonic Model of Cardiac Morphogenesis

Cited by 54 publications

References 49 publications

Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish

Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish

3D Finite Element Electrical Model of Larval Zebrafish ECG Signals

Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope

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