Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Furlan, Maria Montserrat Diaz Pedrosa; Molinari, Sônia Lucy; Neto, Marcílio Hübner de Miranda

doi:10.1590/s0004-282x2002000400012

Cited by 64 publications

(69 citation statements)

References 27 publications

(40 reference statements)

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“…The metabolic abnormalities found (polyuria, polydipsia, polyphagia and weight loss) are consistent with the literature, in the case of diabetic animals (17,39) . When the potential for glycogen storage of the kidneys is exceeded, the high glucose level in blood promotes glucosuria.…”

Section: Discussionsupporting

confidence: 91%

Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

Zanoni

Tronchini

Moure

et al. 2011

Arq. Gastroenterol.

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-Context -Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy.The imbalance between cellular antioxidants and free radicals, leading to an increase in oxidative stress, is considered one of the main factors responsible for neuronal damages in diabetes. Drugs that reduce the oxidative stress may play a significant role in the treatment of neurological complications of diabetes mellitus. Objective -To evaluate the effect of L-glutamine supplementation on the myenteric neurons from the cecum and duodenum of Wistar rats with streptozotocin-induced diabetes mellitus. Methods -The animals were divided in four groups (n = 5): non-treated normoglycemics, normoglycemics treated with L-glutamine, non-treated diabetics and diabetics treated with L-glutamine from the 4th day of diabetes induction on. The amino acid L-glutamine was added to their diet at 1%. Giemsa's technique was employed to stain the myenteric neurons. We determined the cell body area of 500 neurons in each group studied. The quantitative analysis was performed by sampling in an area of 16.6 mm 2 in the cecum and 3.6 mm 2 in the duodenum of each animal. Results -After the supplementation with L-glutamine in the duodenum, we observed a preservation of neuronal density in groups normoglycemic and diabetic (P<0.05). We also observed a preservation of the cell bodies area in diabetic animals (group treated with L-glutamine) (P<0.05). In the cecum, that preservation was not evident. Conclusion -Supplementation with L-glutamine (1%) promoted a neuroprotective effect on the myenteric neurons from the duodenum of rats, both in terms of natural aging and of diabetes mellitus.

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Section: Discussionsupporting

confidence: 91%

Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

Zanoni

Tronchini

Moure

et al. 2011

Arq. Gastroenterol.

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“…Similar results were found by Freitas et al (12) , who observed a significant 37.9% reduction in neuronal density in the jejunum in diabetic animals compared with non-diabetic animals. Other studies performed by our group have analyzed different segments of the gastrointestinal tract, including the ileum (3,22,46) , proximal colon (15,24,25,34,39) , and stomach (14) and showed similar results. This reduction in the number of myenteric neurons results from hyperglycemia, which initiates several biochemical events, such as disturbances in the polyol pathway, formation of AGEs, activation of the protein kinase C and hexosamine pathways, and glycolysis.…”

Section: Discussionsupporting

confidence: 65%

“…Moreover, vitamin E interferes with lipid peroxidation and inhibits the enzyme phospholipase A2, thus preventing membrane phospholipid hydrolysis (15,31,36,43,45) . The present study investigated the effects of vitamin E supplementation at different concentrations (0.1% and 2%) on the general population of myenteric neurons (myosin-V-immunoreactive) and on the subpopulation of nitrergic neurons (neuronal nitric oxide synthase [nNOS]-immunoreactive) in the jejunum of rats with DM.…”

Section: Supplementation With 01% and 2% Vitamin E In Diabetic Rats:mentioning

confidence: 99%

Supplementation with 0.1% and 2% vitamin e in diabetic rats: analysis of myenteric neurons immunostained for myosin-V and nNOS in the jejunum

Tronchini

Trevizan

Tashima

et al. 2012

Arq. Gastroenterol.

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-Context -Diabetes mellitus is a disease characterized by hyperglycemia that, when allowed to progress long-term untreated, develops vascular and neurological complications, which are responsible for the development of alterations in the enteric nervous system in diabetic patients. In the gastrointestinal tract, diabetes mellitus promotes motor and sensory changes, and in the reflex function of this system, causing gastroparesis, diarrhea, constipation, megacolon, slow gastrointestinal transit, gastric stasis and dilation with decreased or increased peristaltic contractions. Several studies have shown that oxidative stress is the main responsible for the vascular and neurological complications affecting the enteric nervous system of diabetics. Objective -The effects of 0.1% and 2% vitamin E on myosin-V-and nNOS-immunoreactive neurons in the jejunum of diabetic rats were investigated. Methods -Thirty rats were divided into the groups: normoglycemic, normoglycemic treated with 0.1% vitamin E, normoglycemic treated with 2% vitamin E, diabetic, diabetic treated with 0.1% vitamin E, and diabetic treated with 2% vitamin E. The neuronal density and areas of neuron cell bodies were determined. Results -Diabetes (diabetic group) significantly reduced the number of myosin-V-immunoreactive neurons compared with the normoglycemic group. The diabetic treated with 0.1% vitamin E and diabetic treated with 2% vitamin E groups did not exhibit a greater density than the D group (P>0.05). Nitrergic density did not change with diabetes (P>0.05). The areas of myosin-V-and nNOS-immunoreactive neurons significantly increased in the normoglycemic treated with 2% vitamin E and diabetic groups compared with the normoglycemic group. Conclusion -Supplementation with 2% vitamin E had a neurotrophic effect only in the area of myosin-V-immunoreactive neurons compared with the diabetic group. HEADINGS -Vitamin E. Diabetes mellitus, experimental. Jejunum. Neurons. Myosin type V. Nitric oxide sinthase. Rats.

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“…A decrease in the number of myenteric neurons in STZ-induced diabetes in rats was observed in the stomach (Fregonesi et al 2001, Clebis et al 2004, in the duodenum (Romano et al 1996, Buttow et al 1997, Furlan et al 1999, in the ileum (Zanoni et al 2003, Alves et al 2006, in the small intestine as a whole (Hernandes et al 2000), in the cecum (Zanoni et al 2007), in the proximal colon (Furlan et al 2002). We can justify the controversial results for the NADPH-dp neurons in Group D, C, CS and DS when compared to other reports: the diabetes maintenance period as well as the neurochemical phenotype of the stained neurons was not similar.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in neurons of the myenteric plexus have been described in different conditions and experimental models which have tried to elucidate the mechanisms that lead to functional impairment of the digestive tube (Fregonesi et al 2001, Furlan et al 2002, Zanoni et al 2003, Fontes et al 2004, Alves et al 2006, Araújo et al 2006, Silverio et al 2008, Silva et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

et al. 2009

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295Pesq. Vet. Bras. 29(4): 295-302, abril 2009 RESUMO.-[Efeitos da suplementação do ácido ascórbico nos neurônios mientéricos do íleo de ratos diabéticos induzidos por estreptozootocina.] A exacerbação do estresse oxidativo e da via do poliol que comprometem o plexo mioentérico são alterações metabóli-cas características do diabetes. O ácido ascórbico (AA) é antioxidante e inibidor da aldose redutase, podendo atuar como neuroprotetor. Verificaram-se os efeitos da suplementação com AA sobre o número e a área do perfil do corpo celular (PC) de neurônios mioentéricos em ratos diabéticos induzidos por estreptozootocina. Formaram-se quatro grupos com cinco animais cada: normoglicêmico (C); diabético (D); diabético tratado com AA (DS); e normoglicêmico tratado com AA (CS). Três vezes por semana administrou-se 50mg de AA para cada animal (grupos DS e CS). Após 90 dias e eutanásia com tiopental, o íleo foi coletado e processado para a técnica da NADPHdiaforase. Não se observaram diferenças (P>0,05) na densidade neuronal entre os grupos. A área do PC foi menor (P<0,05) para os grupos DS e CS, com incidência maior de neurônios com área do PC superior a 200μm 2 para os grupos C e D. Concluiu-se que o AA não influenciou a densidade neuronal do íleo, mas foi neuroprotetor The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200μm 2 for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200μm 2 .INDEX TERMS: Vitamin C, diabetes, intestine, myenteric plexus.

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Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Cited by 64 publications

References 27 publications

Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

Supplementation with 0.1% and 2% vitamin e in diabetic rats: analysis of myenteric neurons immunostained for myosin-V and nNOS in the jejunum

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

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