Morphometric analysis of normal skeletal muscles in infancy, childhood and adolescence

Oertel, G

doi:10.1016/0022-510x(88)90227-4

Cited by 102 publications

(93 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…type I fibers have more total nuclei and satellite cells (Verdijk et al, 2007). Though type I and II fibers from arm and leg muscles have a similar size and grow similarly in both boys and girls up to 15 years of age (Brooke and Engel, 1969;Oertel, 1988), it remains to be established to what extent changes in myonuclear domain size during maturation differ between fiber types and to what extend the loss of satellite cells in infancy is affected by the fiber type of the muscle studied. To test the possibility that changes in fiber type distribution as a function of age interfered with the increase of myonuclear domain, we performed additional stainings and measurements on non-serial sections using the following antibodies: A4.840 (Developmental Studies Hybridoma Bank [DSHB], Department of Biological Sciences, University of Iowa, 028 BBE, Iowa City, USA) a mouse IgM monoclonal antibody raised against myosin heavy chain to visualize human slow (type I) fibers; N2.261, a mouse IgG1 monoclonal antibody raised against myosin heavy chain, to visualize human fast (type IIa) fibers.…”

Section: Discussionmentioning

confidence: 99%

Steep Increase in Myonuclear Domain Size During Infancy

Delhaas

Meer

Schaart

et al. 2012

The Anatomical Record

View full text Add to dashboard Cite

We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 lm 2 Áper annum (R 2 ¼ 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase.

show abstract

Section: Discussionmentioning

confidence: 99%

Steep Increase in Myonuclear Domain Size During Infancy

Delhaas

Meer

Schaart

et al. 2012

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…In fact, some mechanisms can be attributed to explain the obtained values for young people explosive strength during biological development. hey include the largest responses of anabolic hormones, such as testosterone 20 and structural changes in muscle architecture, resulting signiicant changes in type I to type II ibers proportion 21,22 , observed in younger age. Volver et al 23 , further complement that the anabolic androgenic actions in the pubertal period stimulate the development of type II muscular ibers, responsible for explosive force generation.…”

Section: Discussionmentioning

confidence: 99%

Skeletal age and explosive strength in young volleyball players

Gantois

Pinto

Castro

et al. 2017

Rev. Bras. Cineantropom. Desempenho Hum.

View full text Add to dashboard Cite

-Identifying diferent anthropometric, physiological and motor aspects is essential to obtain success in sports practice. However, these components may be developed in same age subjects diferently, in part due biological development. he aim of the study was compare and correlate the explosive strength to biological maturation by sex, obtained by estimation of skeletal age based on anthropometric variables. he sample consisted of 239 subjects of both sexes, aged 10 to 13 years. Maturation was assessed by skeletal age predictive equation and upper and lower limbs explosive strength using medicine ball throw test and vertical jump, respectively. Skeletal age was assessed by skeletal age using a predictive anthropometrical based equation. Upper and lower limbs explosive strength were evaluated by medicine ball throwing test and vertical jump test, respectively. Post hoc analysis showed delayed biological development subjects had worst medicine ball throwing test performance than accelerated boys (p=0.001; d=0.96) and girls (p< 0.01; d= 2.01); regarding to vertical jump test, worst performance was also detected to maturational delayed boys when compared to accelerated ones (p=0.24; d=1.1) and girls (p=0.007; d=0.75). Regression analysis showed skeletal age explained variance of 36% and 19.2% for boys and 45.2% and 16.1% for girls upper and lower limbs explosive strength. Skeletal age is positively related to upper and lower limbs explosive strength and show higher performance for accelerated biological development young players when compared to delayed ones, independently from sex.

show abstract

“…Causes of the observed present age-dependent changes might be changes in muscle cross-sectional area and metabolism during growth that is still discussed as being controversial [17][18][19][20][21]. A comparison of muscular fatigue of boys and of adult men suggests that more fatigable Type 2 fibers are involved in men, resulting in greater lactic acid and ion accumulation during fatigue [18].…”

Section: Discussionmentioning

confidence: 99%

Forearm Oxygen Consumption and Forearm Blood Flow in Healthy Children and Adolescents Measured by Near Infrared Spectroscopy

et al. 2006

View full text Add to dashboard Cite

Abstract:The assessment of forearm oxygen consumption (VO 2 ) and forearm blood flow (FBF) by means of near infrared spectroscopy has become widespread in adults, whereas in children and adolescents no data are available. The aim of the present study was to analyze VO 2 and FBF in healthy children and adolescents. Methods: In a prospective cohort study, 20 male and 20 female healthy children and adolescents were investigated. The measurements of VO 2 and FBF were performed by means of near infrared spectroscopy in combination with the venous occlusion method. Results: Mean VO 2 was 0.08 ± 0.04 ml 100 g -1 min -1 in male and 0.09 ± 0.05 ml 100 g -1 min -1 in female subjects. Mean FBF was 1.95 ± 1.25 ml 100 g -1 min -1 in male and 1.82 ± 0.98 ml 100 g -1 min -1 in female subjects. No significant difference was found between male and female subjects. A significant negative correlation of VO 2 and FBF to age was observed in both groups. Conclusion: In the present study we were able to show that VO 2 and FBF decreased with increasing age in children and adolescents without significant differences between male and female subjects.Key words: near infrared spectroscopy, forearm oxygen consumption, forearm blood flow, development. Near infrared spectroscopy (NIRS) is a relatively newmethod, which is noninvasive and continuous and operates in real time, thus enabling in-vivo monitoring of tissue oxygenation and hemodynamics. NIRS has been applied to measure the oxygenation and hemodynamics of a variety of tissues, including muscle, brain, and connective tissue. With an adequate optode separation (more than 20 mm), the skin and the subcutaneous fat constitutes less than 5% of the signal measured over a given body segment in lean subjects [1,2]. The NIRS signal therefore predominately reflects the microcirculation and O 2 availability of tissue, which is dependent on interoptode distance, 2-6 cm deep [1]. Thus by positioning the optodes on the forearm, NIRS enables the measurement of the oxygenation and hemodynamics of forearm muscle.The venous occlusion, another noninvasive method, in combination with NIRS has been validated and has become an accepted method for the assessment of forearm muscular oxygen consumption (VO 2 ) [3][4][5] and forearm blood flow (FBF) [6]. Nevertheless, most studies on muscle oxygenation and hemodynamics were performed on healthy adults and adult patients [1,4,7] and on term and preterm neonates [8,9]. In a recent study we analyzed FBF in children and adolescents with type I diabetes and healthy children and adolescents [6].The aim of the present study was the measurement and analysis of VO 2 and FBF of forearm muscle in healthy children and adolescents. METHODSChildren and adolescents aged from 6 to 18 years were studied. The subjects, who were obese, i.e., body mass index (BMI) beyond the 90th percentile, or who had taken any medicaments within the past seven days, were excluded. Male and female subjects were matched for age (±6 months). Each subject was a volunteer, and informed consent was ob...

show abstract

Morphometric analysis of normal skeletal muscles in infancy, childhood and adolescence

Cited by 102 publications

References 12 publications

Steep Increase in Myonuclear Domain Size During Infancy

Steep Increase in Myonuclear Domain Size During Infancy

Skeletal age and explosive strength in young volleyball players

Forearm Oxygen Consumption and Forearm Blood Flow in Healthy Children and Adolescents Measured by Near Infrared Spectroscopy

Contact Info

Product

Resources

About