Morphology changes in human lung epithelial cells after exposure to diesel exhaust micron sub particles (PM1.0) and pollen allergens

Esposito, Vincenzo; Lucariello, Angela; Savarese, Leonilde; Cinelli, Mariapia; Ferraraccio, Francesca; Bianco, Andrea; Luca, Antonio De; Mazzarella, Gennaro

doi:10.1016/j.envpol.2012.07.006

Cited by 45 publications

(31 citation statements)

References 21 publications

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“…The relation between asthma and residence zone was investigated, but relevant differences were found only for school and residing location in an urban or suburban area. A significant association with outcome was found for asthmatic children/adolescents living in the municipality of Termoli that may be subjected to higher environmental pressure due to industrial/manufacturing activities and presence of toll road, state highway, railroad, and seaport which may cause air pollution from motor vehicle traffic and increase asthma induction, according to other studies [19,41,42]. …”

Section: Discussionmentioning

confidence: 71%

Asthma prevalence and risk factors among children and adolescents living around an industrial area: a cross-sectional study

et al. 2013

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BackgroundThe exposure to air pollution has negative effects on human health, increasing the risk of respiratory diseases, such as asthma. Few data are yet available on the epidemiology of childhood asthma in some areas of Italy. The aim of the study was to estimate asthma prevalence and related risk factors in children and adolescents residents around the industrial area of Termoli, Molise region, Central-South Italy.MethodsPrevalence was assessed through the administration of modified ISAAC questionnaires filled out by parents of 89 children and adolescents for the identification of confirmed and probable cases, and by analyzing pediatricians’ databases on drug prescriptions for symptoms control and treatment of assisted population in the study area (n = 1,004), compared to a control area (n = 920) with lower industrialization. The association of asthma with risk factors was evaluated by univariate (Chi-square or Fisher’s Exact test) and regression logistic analysis.ResultsA total of 22 (24.7%) asthmatics were identified, including both confirmed (n = 7; 7.9%) and probable cases (n = 15; 16.8%), most of them (n = 17; 77.3%) resident of Termoli town. All asthma cases were georeferenced based on the residence, however clusters were not found. Using drug prescriptions analysis, a higher prevalence (n = 138; 13.7%) of diagnosed cases was found. Lifetime history of both atopic dermatitis and bronchitis were significantly relateds to asthma cases, as well as an elevated body mass index, whose association is consistent with prevalence data of overweight/obese children living in the study area. Moreover, being resident of the town of Termoli was associated to the occurrence of cases.ConclusionsAlthough our data indicated a prevalence concordance with previous national studies in pediatric population, a definitive correlation with environmental industrial factors present in the study area was not established. However, asthma outcome was significantly associated to individuals living in the town of Termoli that, despite the industrial/manufacturing activities, is also subjected to a higher environmental pressure due to the presence of toll road, state highway, railroad, and seaport which may cause air pollution from motor vehicle traffic and increase asthma induction. This study provides hitherto unavailable data on asthma in childhood population living in an industrialized area which was never investigated before, could be part of a systematic review or meta-analysis procedure, might suggest significant findings for larger observational studies, and contribute to complete the frame of disease epidemiology in Italy.

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Section: Discussionmentioning

confidence: 71%

Asthma prevalence and risk factors among children and adolescents living around an industrial area: a cross-sectional study

et al. 2013

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“…Wang & Petersen (2013) showed that lipid-coated Au NPs promoted LB formation. An increase of LBs was also reported by Esposito et al (2012); however, for sub-micron particles (PM1.0). Similar to the observations made by Wang & Petersen (2013), who found Au NPs in MVBs and LBs, our TEM examination revealed that A549 cells internalize SiO 2 -SPIONs predominantly inside MVBs and LBs.…”

Section: Discussionmentioning

confidence: 88%

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

et al. 2017

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The pulmonary delivery of nanoparticles (NPs) is a promising approach in nanomedicine. For the efficient and safe use of inhalable NPs, understanding of NP interference with lung surfactant metabolism is needed. Lung surfactant is predominantly a phospholipid substance, synthesized in alveolar type II cells (ATII), where it is packed in special organelles, lamellar bodies (LBs). In vitro and in vivo studies have reported NPs impact on surfactant homeostasis, but this phenomenon has not yet been sufficiently examined. We showed that in ATII-like A549 human lung cancer cells, silica-coated superparamagnetic iron oxide NPs (SiO 2 -SPIONs), which have a high potential in medicine, caused an increased cellular amount of acid organelles and phospholipids. In SiO 2 -SPION treated cells, we observed elevated cellular quantity of multivesicular bodies (MVBs), organelles involved in LB biogenesis. In spite of the results indicating increased surfactant production, the cellular quantity of LBs was surprisingly diminished and the majority of the remaining LBs were filled with SiO 2 -SPIONs. Additionally, LBs were detected inside abundant autophagic vacuoles (AVs) and obviously destined for degradation. We also observed time-and dose-dependent changes in mRNA expression for proteins involved in lipid metabolism. Our results demonstrate that non-cytotoxic concentrations of SiO 2 -SPIONs interfere with surfactant metabolism and LB biogenesis, leading to disturbed ability to reduce hypophase surface tension. To ensure the safe use of NPs for pulmonary delivery, we propose that potential NP interference with LB biogenesis is obligatorily taken into account. ARTICLE HISTORY

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“…In addition, an increase in the number of dead cells was observed in these treatment groups. The different mineral granule treatments caused varying degrees of morphological damage (Esposito et al, 2012;Al-Hallak et al, 2012;Meenach et al, 2014). Montmorillonite and quartz treatment caused the most widespread destruction of the cell morphology (Xia et al, 2014;Sharma et al, 2014), whereas albite and sericite treatment caused the least amount of cell damage (Discoll et al, 1998;Ghio et al, 2014;Ziemann et al, 2014).…”

Section: The Influence Of Minerals On the Morphology Of Cellmentioning

confidence: 99%

Assessment of genetic toxicity with major inhalable mineral granules in A549 cells

Zeng

Deng

Huo

et al. 2016

Applied Clay Science

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Morphology changes in human lung epithelial cells after exposure to diesel exhaust micron sub particles (PM1.0) and pollen allergens

Cited by 45 publications

References 21 publications

Asthma prevalence and risk factors among children and adolescents living around an industrial area: a cross-sectional study

Asthma prevalence and risk factors among children and adolescents living around an industrial area: a cross-sectional study

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Assessment of genetic toxicity with major inhalable mineral granules in A549 cells

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Morphology changes in human lung epithelial cells after exposure to diesel exhaust micron sub particles (PM1.0) and pollen allergens

Cited by 45 publications

References 21 publications

Asthma prevalence and risk factors among children and adolescents living around an industrial area: a cross-sectional study

Asthma prevalence and risk factors among children and adolescents living around an industrial area: a cross-sectional study

Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Assessment of genetic toxicity with major inhalable mineral granules in A549 cells

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Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells