Assessment of genetic toxicity with major inhalable mineral granules in A549 cells

Zeng, Yiming; Deng, Jianjun; Huo, Tingting; Dong, Faqin; Wang, Limin

doi:10.1016/j.clay.2015.08.035

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…As dust concentration increases the number and sizes of the colonies decrease (Fig. 2B-C), supporting previous studies showing that dust suppresses the growth and survival of human cells (Zeng et al, 2016;Yang et al, 2017).…”

Section: Exposure To Dust Reduces Cell Viability But Leads To Cell Death In a Non-monotonic Waysupporting

confidence: 89%

“…Exposure to dust particles is known to affect cells' viability. Previous studies investigated this by focusing on cell populations, and quantifying thousands of cells at single fixed timepoints at usually 24 h (Yamada et al, 2012;Chang et al, 2016;Pardo et al, 2017) or 48 h (Deng et al, 2007;Zeng et al, 2016) post dust exposure. In this work, we used live imaging to follow individual cells over time in response to a wide range of dust concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…It was also shown that an increase in dust concentrations leads to a decrease in cell viability (Veranth et al, 2004(Veranth et al, , 2006Rodríguez-Cotto et al, 2013;Chang et al, 2016). Recent studies examined the effect of dust particles on live cells and discovered that an increase in dust concentrations inhibit cell proliferation (Deng et al, 2007;Yang et al, 2017;Zeng et al, 2016). Note that these studies examined the exposure to dust at a single time point and in a bulk population of cells (Yamada et al, 2012;Ortiz-Martínez et al, 2015) masking the dynamics of cell growth and death in single cells and the heterogeneity between cells.…”

Section: Introductionmentioning

confidence: 99%

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The effect of dust storm particles on single human lung cancer cells

Ardon‐Dryer

Mock

Reyes

et al. 2020

Environmental Research

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Exposure to dust particles during dust storms can lead to respiratory problems, diseases, and even death. The effect of dust particles at the cellular level is poorly understood. In this study, we investigated the impact that dust storm particles (Montmorillonite) have on human lung epithelial cells (A549) at the single cell level. Using live-cell imaging, we continuously followed individual cells after exposure to a wide range of concentrations of dust particles. We monitored the growth trajectory of each cell including number and timing of divisions, interaction with the dust particles, as well as time and mechanism of cell death. We found that individual cells show different cellular fates (survival or death) even in response to the same dust concentration. Cells that died interacted with dust particles for longer times, and engulfed more dust particles, compared with surviving cells. While higher dust concentrations reduced viability in a dose-dependent manner, the effect on cell death was non-monotonic, with intermediate dust concentration leading to a larger fraction of dying cells compared to lower and higher concentrations. This non-monotonic relationship was explained by our findings that high dust concentrations inhibit cell proliferation. Using cellular morphological features, supported by immunoblots and proinflammatory cytokines, we determined that apoptosis is the dominant death mechanism at low dust concentrations, while higher dust concentrations activate necrosis. Similar single cell approaches can serve as a baseline for evaluating other aerosol types that will improve our understanding of the health-related consequences of exposure to dust storms.

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Section: Exposure To Dust Reduces Cell Viability But Leads To Cell Death In a Non-monotonic Waysupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of dust storm particles on single human lung cancer cells

Ardon‐Dryer

Mock

Reyes

et al. 2020

Environmental Research

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show abstract

“…Interestingly, Zhou and co-workers (Yan et al, 2015, in this issue) describe zinc-bearing palygorskite which was used as an additive to the feed for chicken and discover that such a form of Zn in the feed had a higher bioavailability than single ZnSO 4 as an additive to the feed. Zeng et al (2015, in this issue) assess the genotoxicity of inhalable clay mineral particulate matter (PM) in A549 cells.…”

Section: Advanced Materials and Practical Applicationsmentioning

confidence: 99%

Current fundamental and applied research into clay minerals in China

Zhou

Zhao

Wang

et al. 2016

Applied Clay Science

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“…Estudos do efeito genotóxico de material particulado de ar em áreas urbanas utilizando o teste de micronúcleo foram realizados com diversos organismos e tipos celulares, como vegetais do gênero Tradescantia (Carreras et al, 2013;Ceretti et al, 2015), células das raízes de Vicia faba (Corrêa et al, 2016), linhagem celular de macrófagos RAW264.7 (Poma et al, 2006), células de carcinoma de pulmão humano (Roubicek et al, 2007;Zeng et al, 2015), células BEAS--2B de epitélio brônquico humano (Oh et al, 2011) e linfócitos de sangue periférico humano (O'Callaghan--Gordo et al, 2015).…”

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Genotoxicidade <i>in vitro</i> das frações orgânica e solúvel em água de material particulado de ar em três locais do Estado de São Paulo

Betancur¹,

Cristina²

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Geno to xicid ad e in vitro d as fraçõ es o rgânic a e so lúvel em água de mater ial p articulad o d e ar em três lo cais d o Estad o d e São Paulo / Isabel Cristina Palacio Betancur.-São Paulo, 2016. 1 32p. T ese (d o uto r ad o)-Faculd ad e d e Ciências Farmacêuticas da Univer sid ad e d e São P aul o. Dep artamento d e Análises Clínicas e T o xico ló gicas. Or ientad o r : B ar ro s, Silvia B erlanga d e Mo raes Coo r ientad o r : Ro ub icek, Deb o rah Arnsd o rff 1. P o luição amb iental : T o xico lo gia I. T. II. Mo raes, Silvia B er langa de, o r ientad o r. III. Ro ub icek, Deb o ra h Arnsd o rff, coo r ientad o r 615.9 CDD

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Assessment of genetic toxicity with major inhalable mineral granules in A549 cells

Cited by 8 publications

References 42 publications

The effect of dust storm particles on single human lung cancer cells

The effect of dust storm particles on single human lung cancer cells

Current fundamental and applied research into clay minerals in China

Genotoxicidade <i>in vitro</i> das frações orgânica e solúvel em água de material particulado de ar em três locais do Estado de São Paulo

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