Morphological identification of Ilyonectria liriodendri and its interaction with plant growth-promoting bacteria in grapevine rootstocks

Abstract

In order to analyze the pathogenicity and antagonism between grapevine rootstocks and soil microorganisms, plant growth-promoting bacteria with antibiotic effect were selected from the phytopathogen Ilyonectria liriodendri, 2T-VSC-101 isolated monosporic, in in vitro antibiosis tests. The bacterial access that produced the largest zone of inhibition was used in the seedling biotization of the grapevine rootstocks P1103, IBBT481 and VR039-16. From the interaction between isolates of plant growth-promoting bacteria and Ilyonectria liriodendri isolate resulted in three bacterial isolates with control of the mycelial growth of the phytopathogen, all of the Burkholderia gender. However, the highest control bacterium, Burkholderia catarinensis sp. nov. isolated POA 89, was found to be detrimental to the in vitro development of rootstocks, causing them to die. The cultivation through culture medium modified with sand was adequate for the in vitro growth of the rootstocks and appropriate for the observation of the black foot disease's characteristic symptoms. From the ex vitro cultures with the IBBT481 rootstock and the microorganisms, there were results in favorable effects to the plants inoculated with the bacterium in combination with the phytopathogen. It also demonstrated the difficulty of acclimatization and growth of this access in a greenhouse. Identificação morfológica de Ilyonectria liriodendri e sua interação com bactérias promotoras de crescimento de plantas em porta enxertos de videiraResumo -Com a finalidade de analisar a patogenicidade e o antagonismo entre porta-enxertos de videiras e microorganismos de solo, procedeu-se à seleção de bactérias promotoras de crescimento de plantas com efeito antibiótico ao fitopatógeno Ilyonectria liriodendri, isolado monospórico 2T-VSC-101, em testes de antibiose in vitro. O acesso bacteriano que produziu a maior zona de inibição foi utilizado na biotização de mudas dos porta-enxertos de videira P1103, IBBT481 e VR039-16. Da interação entre isolados de bactérias promotoras de crescimento de plantas e o fungo causador da doença-pé-preto-da-videira, resultaram, três isolados bacterianos com controle sobre o crescimento micelial do fitopatógeno, todos do gênero Burkholderia sp. Entretanto, a bactéria de maior controle, Burkholderia catarinensis sp. nov. isolado POA 89, mostrou-se prejudicial ao desenvolvimento in vitro dos porta-enxertos, causando-lhes a morte. O cultivo com meio de cultura modificado com pedrisco mostrou-se adequado ao crescimento in vitro dos porta-enxertos e apropriado à observação dos sintomas característicos da doença pé-preto. Das culturas ex vitro com o porta-enxerto IBBT481 e os microorganismos, resultaram efeitos favoráveis às plantas inoculadas com a bactéria em combinação com o fitopatógeno. Também demonstrou a dificuldade de aclimatização e de crescimento desse acesso em casa de vegetação. Termos para indexação: Micropropagação, aclimatização, Burkholderia catarinensis sp. nov., Ilyonectria liriodendri.

Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

“…The controls (CK) were treated with an equal volume of sterile water; three biological replicates of each treatment were set up for all experiments. The seedlings were cultivated at 25°C, 3,000 lx, 12-h light/dark photoperiod for 30 days ( de Brito et al 2019 ). Three replicates were made per treatment, and the experiment was repeated two times.…”

The functional microbiome of grapevine throughout plant evolutionary history and lifetime

“…(I. liriodendri, in particular), is responsible for root necrotic lesions, with a reduction in root biomass, besides other symptoms that include reduction of internodes, loss of vigor, patchy foliage and smaller leaves (Bleach et al, 2021). The fungal genus Ilyonectria is found in many metabarcoding analyses of vineyard soil because it can persist for several years after the removal of infected vines (Brito et al, 2019;Deyett and Rolshausen, 2020;Liu and Howell, 2021;Rivas et al, 2022). Several fungal genera associated with grapevine trunk diseases (Botryosphaeria and Diplodia; Section 2.5), Petri disease (Cadophora, Phaeoacremonium and Phaeomoniella) and Diaporthe dieback (Diaporthe) have been detected in asymptomatic root tissues (Deyett and Rolshausen, 2020;Liu and Howell, 2021;Martínez-Diz et al, 2019;Rivas et al, 2022).…”

Development and application of a droplet digital PCR assay for the detection of Fusarium oxysporum f. sp. cubense tropical race 4 from different biological samples

“…The ddPCR or third‐generation PCR has some advantages over qPCR, including that no standard curve is required for quantification, resilience to PCR inhibitory substances sometimes present in environmental samples and higher sensitivity and accuracy in the quantification of target DNA (Dingle et al., 2013; Falzone et al., 2020; Koepfli et al., 2016; Rački et al., 2014; Singh et al., 2017; Tang et al., 2016; Wang et al., 2019). To date, ddPCR has been used as a methodology for the diagnosis of microorganisms affecting different crops, including soil‐inhabiting fungi (Blaya et al., 2016; de Brito et al., 2019; Liu et al., 2020; Zhao et al., 2016).…”