Morphological Examination and Transcriptomic Profiling To Identify Prednisolone Treatment in Beef Cattle

Cannizzo, Francesca Tiziana; Pegolo, Sara; Pregel, Paola; Manuali, Elisabetta; Salamida, Sonia; Divari, Sara; Scaglione, Frine Eleonora; Bollo, E.; Biolatti, B.; Bargelloni, Luca

doi:10.1021/acs.jafc.6b02996

Cited by 5 publications

(6 citation statements)

References 51 publications

(122 reference statements)

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“…Moreover, PDN residues were not detected in the urine of treated animals likely because of the rapid metabolism of this CS. PDN is still used in livestock production because of its capacity to increase weight gains. , …”

Section: Resultsmentioning

confidence: 99%

“…PDN is still used in livestock production because of its capacity to increase weight gains. 17,18 For these reasons, it is even more pressing to identify biomarkers of PDN treatment in cattle to discriminate exogenous administration of this CS for illicit financial profit.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Animals and Experimental Design. The experimental design has been described previously by Cannizzo et al 6,17,18 For this study, in particular, 12 male Italian Friesian beef cattle (9−17 months old) were considered. They were randomly divided into two groups: group P (n = 6) was treated with 30 mg day −1 PDN acetate (PA, Novosterol, Italy) per os for 35 days, and group C (n = 6) was the control group that received a placebo.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The animal weight gain was recorded, as previously reported by Cannizzo et al 17,18 Tissue Sampling and Processing. Thymus glands were collected, weighed, and histologically examined as previously reported by Cannizzo et al 6 Samples of both VAT and SCAT were taken from each animal during slaughtering, frozen in liquid nitrogen, and stored at −80 °C.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

Divari

Berio

Biolatti

et al. 2017

J. Agric. Food Chem.

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Corticosteroids are frequently used in livestock production, and their use is permitted by the European Union for therapeutic purposes only. However, small doses of corticosteroids are often administered in meat-producing animals to improve zootechnical performance. Prednisolone is one of the most commonly used corticosteroids with a growth-promoting purpose in animal husbandry. This study proposes to identify a gene whose expression is significantly regulated by prednisolone in visceral and subcutaneous adipose tissues. The analysis was conducted on Friesian cattle treated with prednisolone (30 mg day). The reference gene expression stability and optimal number for gene expression normalization were calculated. Family with sequence similarity 107 member A (FAM107A) and pyruvate dehydrogenase kinase 4 are the prednisolone target genes identified in adipose tissue. FAM107A was downregulated by ∼2.9-fold by prednisolone in subcutaneous adipose tissue. This result suggests that FAM107A could be a possible indirect biomarker of prednisolone treatment in cattle and encourages a deeper investigation in this direction.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

Divari

Berio

Biolatti

et al. 2017

J. Agric. Food Chem.

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“…The collection of other matrices requires direct contact with the animal. A good example of this is blood, which is commonly used for in vivo transcriptomics assays [ 19 ]. Another option to carry out in vivo transcriptomics assays is the hair follicle, as hair is a common matrix to detect the use of illicit substances in cattle [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison

et al. 2018

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The use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden in the European Union. Due to the very low circulating concentration of rbST in treated animals, its direct detection is still a challenge. Therefore, the use of indirect methods to detect the ab(use) of rbST in dairy cattle appears as a good alternative. In the past few years, gene expression demonstrated its utility in screening the use of illicit substances in both humans and animals. In this study, a comparison of three types of matrices (milk somatic cells, blood, and hair follicles) was carried out to evaluate their potential use for routine control of rbST using 15 gene-expression profiles. A total of six rbST-treated cows and three control cows were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group. Samples of the three matrices were collected before rbST administration, and at three and nine days after treatment. The quality of RNA extracted was higher in the blood and hair-follicle samples than in the milk somatic cells. In the three matrices, there were significant differences in the expression of some genes, with milk somatic cells and blood presenting the the best matrices. On this note, the cyclin D1 (CCND1), interleukin 1 beta (IL-1β), tumor necrosis factor (TNF), and insulin-like growth factor 1 receptor (IGF-1R) genes showed potential as biomarkers of rbST treatment. Therefore, blood, somatic cells, and follicle hair should be considered as promising sources of RNA, and can be used in gene-expression assays to routinely control the illicit use of rbST.

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Influence and Detection of the Residues of Veterinary Formulations in Foods

Regal

Lamas

Fente

et al. 2021

Comprehensive Foodomics

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Morphological Examination and Transcriptomic Profiling To Identify Prednisolone Treatment in Beef Cattle

Cited by 5 publications

References 51 publications

Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison

Influence and Detection of the Residues of Veterinary Formulations in Foods

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