Morphological characters and SRAP analysis of two hybrids between Hibiscus dasycalyx and Hibiscus ‘Moy Grande’

Yu, Chang Yeon; Yin, Yongguang; Creech, David; Lü, Zhiguo

doi:10.1016/j.scienta.2015.11.037

Cited by 6 publications

(7 citation statements)

References 15 publications

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“…SRAP markers amplify open reading frames and display a co-dominant inheritance mode, which makes it easier for hybrid identification. This hybrid identification method could further be augmented with additional morphological variability as has been previously demonstrated for C. oleifera (Fan et al 2011;Peng S et al 2011;Peng F 2012;Huang et al 2014;Abbas et al 2015;Yu et al 2016;. In the present study, we used 17 SRAP primes which resulted in stable amplification and produced unambiguous highly polymorphic DNA fragments (average % polymorphic bands (PPB) across all accessions = 91.60 % (Table 2)).…”

Section: Yield and Fruit Indicators Relationship With Genetic Distancmentioning

confidence: 76%

“…Single primer or primer combinations diagnosed parental and hybrid plants. According to the method of Huang et al (2014) and Yu et al (2016), hybrids were successfully determined if they share bands exclusively amplified in male parents. However, when single primers were used, hybrid identification success rate varied across primers and ranged between 37.50 and 87.50 % for Me3-Em8 and Me3-Em7, respectively, with an overall average of 60.29 % ( Supplementary Table 1).…”

Section: Oleifera Hybrid Identificationmentioning

confidence: 99%

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Marker-assisted selection in C. oleifera hybrid population

et al. 2020

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Marker-assisted selection (MAS) is implemented to improve Camellia oleifera yield and fruit attributes for meeting China’s increased demand for new varieties development. We conducted three-generational (G0, G1, and G2) hybridization (diallel mating) and selection experiment and used Sequence-Related Amplified Polymorphism (SRAP). SRAP markers to investigate their utility in a MAS framework. The utilized SRAP markers were instrumental in hybrid authenticity and the identification of matroclinal or patroclinal inheritance presence, thus guiding mating pair selection and direction (their role as male or females). Across the studied 3 generations, estimates of genetic diversity parameters showed steady increase with percentage increase of ((G0 to G1 and G1 to G2) 9.25 and 9.05: observed number of alleles; 3.12 and 7.80: means effective number of alleles; 12.35 and 22.34: Nei‘s gene diversity; and 14.21 and 21.77: Shannon‘s index), indicating lack of diversity reduction associated with selection. Estimates of genetic distance and their correlation with heterosis were useful in guiding selection of mating pairs for achieving the desired yield and fruit attributes (fruit diameter, height, weight, and index, peel thickness, number of seeds per fruit, seed weight per fruit, and seed rate). Most yield and fruit attributes exhibited high broad-sense heritability with increasing trend over generation intervals, indicating the increased potential of hybrid breeding for this species.

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Section: Yield and Fruit Indicators Relationship With Genetic Distancmentioning

confidence: 76%

Section: Oleifera Hybrid Identificationmentioning

confidence: 99%

Marker-assisted selection in C. oleifera hybrid population

et al. 2020

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“…Some have found high associations between molecular and morphological markers in ornamental plants. Morphological character and sequence-related amplified polymorphism (SRAP) analyses of two hybrids between Hibiscus dasycalyx and Hibiscus 'Moy Grande' were preformed, and the hybrids showed hybrid vigor, and intermediate flower and leaf morphology compared with the parent plants and a higher genetic similarity coefficient with the female parent (H. dasycalyx) than with the male parent (H. 'Moy Grande') (Yu et al, 2016). Diversity analysis based on agromorphological traits and microsatellitebased markers were also used in global germplasm collections of roselle (Hibiscus sabdariffa L.).…”

Section: Resultsmentioning

confidence: 99%

Morphological and Molecular Analyses of Reciprocal Hybrids between ‘Slim Whitman’ and ‘Pinza’, Two Narcissus pseudonarcissus Cultivars

Tang

Shi

2017

horts

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Narcissus pseudonarcissus, also known as daffodil, is a world-famous ornamental flower. In this study, for the first time, cross-pollinations between two widely cultivated N. pseudonarcissus varieties ‘Slim Whitman’ and ‘Pinza’ were performed. After eight consecutive years of cultivation, 27 reciprocal hybrids with different genotypes survived; 15 hybrids in ‘Slim Whitman’ × ‘Pinza’ and 12 in ‘Pinza’ × ‘Slim Whitman’. Twenty ornamental and agronomic characters were observed to evaluate the pattern and extent of genetic variability of the hybrids and relatedness with their parents. The hybrids showed great variation in most morphological characters compared with the parents, especially in leaf and flower characters. Hybrids SP03, SP04, SP05, SP12, SP13, PS04, PS06, PS07, PS08, and PS11 had evident growth advantage in some aspects compared with both parents. Of these hybrids, SP04 got novel flowers with white petals and an yellow-orange corona, and had the potential to become a new popular N. pseudonarcissus cultivar. Hybrids SP01, SP03, SP05, PS04, PS06, and PS07 also possessed a great ornamental value. Using cluster analysis based on morphological traits and random amplified polymorphic DNA (RAPD) molecular markers, genetic relationships among the reciprocal cross hybrids and their parents were further analyzed. The 27 reciprocal hybrids and their parents grouped into divergent clusters, showing that there was rich genetic variation among the hybrids tested. This study will pave the way for hybridization breeding programs of N. pseudonarcissus.

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“…18 All MIPs (Major Intrinsic Proteins) include two highly conserved NPA (Asn-Pro-Ala) motifs that play a crucial role in selective water conduction in aquaporin water channels. 19 Recent developments in molecular biology have resulted in the development of molecular markers such as RAPD (Randomly Amplified polymorphic DNA), 20 AFLP (Amplified Fragment Length Polymorphism), 21 SSR(Simple Sequence Repeat), 22 ISSR (Inter-Simple Sequence Repeat), 20 SRAP (Sequence Related Amplified Polymorphism), 23 that are used in various studies like, genetic diversity, fingerprinting and elucidation of the genetic origin of various species. Due to their many advantages, including co-dominance, high polymorphism, relative abundance and reproducibility, efficiency and simplicity, SSR markers were considered relevant markers for several studies such as plant cultivar identification, genetic linkage map construction, genetic diversity analysis, and comparative genomics.…”

Section: Introductionmentioning

confidence: 99%

In Silico development of new SSRs primer for aquaporin linked to drought tolerance in plants

Karim

Gaboun²,

Belkadi

et al. 2018

Plant Signaling & Behavior

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Plants are exposed to various stress factors including biotic and abiotic stresses. Drought is a limiting factor that minimizes the development and growth of several plants in arid and semi-arid regions. Stress response is usually occur at different levels, Morphological, physiological and biochemical while at the molecular level a large number of genes are involved. This study aims at developing a new SSR primer for aquaporin related to drought stress in plants. A total of 177 complete coding sequences (CDS) available in the NCBI database are downloaded. After analyzing with BLAST, 163 sequences are selected. 1294 SSR derived from these sequences are characterized with MISA and indicating that all sequences contained SSRs. The most abundant SSR has been tetra-nucleotide repeat motif (36%) and among all the tetra-nucleotide repeats, the motif AAAG/CTTT was the most common type, whereas in tri-nucleotide, the motif CCG/CGG has been the predominate type. By using Primer3, 1120 primer pairs are generated and after analyzing, only 735 non redundant primer pairs that present the good characteristics are selected. Among them, some of the pairs of primers are randomly selected and validated on DNA of various species using PCR and agarose gel. ARTICLE HISTORY

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Morphological characters and SRAP analysis of two hybrids between Hibiscus dasycalyx and Hibiscus ‘Moy Grande’

Cited by 6 publications

References 15 publications

Marker-assisted selection in C. oleifera hybrid population

Marker-assisted selection in C. oleifera hybrid population

Morphological and Molecular Analyses of Reciprocal Hybrids between ‘Slim Whitman’ and ‘Pinza’, Two Narcissus pseudonarcissus Cultivars

In Silico development of new SSRs primer for aquaporin linked to drought tolerance in plants

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