Narcissus is widely used for cut flowers and potted plants, and is one of the most important commercial bulbous flowers in the floricultural industry. In this study, ten carotenoid and eighteen flavonoid compounds from the perianths and coronas of fifteen narcissus cultivars were measured by HPLC–APCI-MS/MS and UPLC-Q-TOF-MS/MS. Among these, six carotenoids, a total of seventeen flavonols and chlorogenic acid were identified in narcissus for the first time. A multivariate analysis was used to explore the relationship between flower color and pigment composition. We found that all-trans-violaxanthin and total carotenoid content were the main factors that affected flower color. These investigations could provide a global view of flower color formation and a theoretical basis for hybridization breeding in narcissus.

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Based on published reports and direct observation, the common resources of landscape plant species in the Shanghai area were identified and studied in terms of the landscape characteristics of timing sequence changes over a one year time period. The results showed that there were 199 common landscape plant species in Shanghai, distributed in 67 families and 129 genera. These plants were categorized by three ornamental characteristics: flowers, foliage and fruits. The species were thus further analyzed according to the time periods which these characteristics were present. The results showed that flower ornamental distributed throughout the year, particularly from April to June, and plant species with colorful foliage could be seen every month, and particularly in November and December. On the other hand, few plant species with fruit ornamentals were observed in Shanghai area. The diversity of landscape timing sequence changes of the 199 plant species under examination was then analyzed by using the Shannon-Weiner and Simpson index formulas. The results showed that the higher diversity was observed in January, February and from August to October, while the lowest diversity was recorded from March to July and during November and December. The diversity of landscape characteristics of these plants was ranked according to foliage, flowers and fruit. Using this method, one park and one plant community were selected to evaluate the advantages and disadvantages of their time-changing plant landscape, with the goal of assessing the validity of the chosen ranking method. These results in the present study would be helpful to evaluate the plant landscape in a specified space and further enrich the diversity of plant and landscape by a target-oriented way.Modern urban landscaping brings ecological and social benefits to city residents, including direct contact with seasonal scenery changes. Landscape plants are the main elements for these timing sequence changes, and their annual life rhythms provide the basis for developing harmonious landscaping [1] . Indeed, timing sequence changes of landscape plants is the key factor for enriching garden diversity, as emphasis on diverse seasonal changes of landscape plants is an important way to improve the quality of urban green spaces. Up to now, there were many reports on the importance and evaluation about the seasonal changes of plants around the world [2][3] .In the past two decades, landscaping has made significant achievements in improving the public parks in Shanghai. Specifically, considerable effort has been made to increase the seasonal changes and introduce the diverse colorful plant species in green space. For example, through selecting and domesticating a variety of colorful plant and shrub species not indigenous to the Shanghai area, the diversity of foliage ornamentals has increased [4][5] . However, this process requires long-term research and practice so as to achieve a properly multifaceted enriching diversity of landscape plants in Shanghai. It is therefore import...

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Followed a heat acclimation pretreatment, seedlings of Freesia hybrida 'Shangnong Jinghuanghou' were exposed to heat stress at 38°C for 6 h treatment and then recovered at 22°C for 72 h to study the impact of heat acclimation (30°C) on thermotolerance under heat stress. The results showed that the pretreated seedlings performed better under heat stress than control. Heat acclimation could slow down the decrease of chlorophyll contents under heat stress and recover better. Higher levels of soluble sugar and proline and slight lower level of soluble protein were observed in pretreated seedlings. After recovery, similar levels of proline and soluble protein were maintained in all seedlings. However, a higher level of soluble sugar was maintained in pretreated seedlings. MDA content and EL showed a stable level in pretreated seedlings while a significant increase in control, followed by a significant decrease after recovery. Significant different responses of SOD, POD, CAT, and APX activities were observed in pretreated seedlings and control. Heat acclimation led to higher activities of these enzymes and a significant response of antioxidant enzyme activities occurred in a time-dependent manner under heat stress. Exposure to high temperature caused a significant increase in SOD and APX activity, and much higher levels in SOD and APX activity were observed in pretreated seedlings compared to control during heat stress. A slight difference in change pattern of POD and CAT activity was presented. The highest activities of POD and CAT were observed at 4 and 6 h of heat stress in pretreated seedlings and control, respectively. After 72 h recovery, the activities of all tested enzymes decreased to similar levels in all seedlings.

Water stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. In this study, the Brassica oleracea var. acephala BoRS1 gene was transferred into tobacco through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis, Southern hybridization and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under water stress, and physiological responses to water stress of transgenic tobacco were studied. The results showed that the transgenic plants exhibited better growth status under water stress condition compared to the untransformed control plants. In physiological assessment of water tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of water stress than the untransformed control plants. This study shows that BoRS1 is a candidate gene in the engineering of crops for enhanced water stress tolerance.

Narcissus pseudonarcissus is an important bulbous plant with white or yellow perianths and light yellow to orange-red coronas, but little is known regarding the biochemical and molecular basis related to flower color polymorphisms. To investigate the mechanism of color formation, RNA-Seq of flower of two widely cultured cultivars (‘Slim Whitman’ and ‘Pinza’) with different flower color was performed. A total of 84,463 unigenes were generated from the perianths and coronas. By parallel metabolomic and transcriptomic analyses, we provide an overview of carotenoid biosynthesis, degradation, and accumulation in N. pseudonarcissus. The results showed that the content of carotenoids in the corona was higher than that in the perianth in both cultivars. Accordingly, phytoene synthase (PSY) transcripts have a higher abundance in the coronas than that in perianths. While the expression levels of carotenoid biosynthetic genes, like GGPPS, PSY, and LCY-e, were not significantly different between two cultivars. In contrast, the carotenoid degradation gene NpCCD4 was highly expressed in white-perianth cultivars, but was hardly detected in yellow-perianth cultivars. Silencing of NpCCD4 resulted in a significant increase in carotenoid accumulation, especially in all-trans-β-carotene. Therefore, we presume that NpCCD4 is a crucial factor that causes the low carotenoid content and color fading phenomenon of ‘Slim Whitman’ by mediating carotenoid turnover. Our findings provide mass RNA-seq data and new insights into carotenoid metabolism in N. pseudonarcissus.

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