Morphological and Morphometric Analysis on the Rabbit Conjunctival Goblet Cells in Different Hormonal Conditions

Aragona, Pasquale; Puzzolo, Domenico; Micali, Antonio; Ferreri, Giuseppe; Britti, Domenico

doi:10.1006/exer.1997.0406

Cited by 19 publications

(19 citation statements)

References 28 publications

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“…the goblet cell profile in our micrographs was usually rather squat or even elongated in the plane parallel to the ocular surface, as opposed to being tall and elongated in a plane perpendicular to the ocular surface (e.g. Aragona et al, 1998) …”

Section: Discussionmentioning

confidence: 96%

“…The surface cells appear to be the same as the type II cells reported extensively in other TEM studies of the conjunctival epithelium (Rohen, 1986 ;Rohen and Steuhl, 1982 ;Steuhl and Rohen, 1984 ;Steuhl and Knorr, 1990). The more electron-lucent granules and\or those with an electron dense core may represent a different stage of a synthetic and secretory cycle of these granules, analogous to differences in goblet cell granule density (Aragona et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

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The Acinar and Ductal Organisation of the Tarsal Accessory Lacrimal Gland of Wolfring in Rabbit Eyelid

Bergmanson

Doughty

Blocker

1999

Experimental Eye Research

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

The Acinar and Ductal Organisation of the Tarsal Accessory Lacrimal Gland of Wolfring in Rabbit Eyelid

Bergmanson

Doughty

Blocker

1999

Experimental Eye Research

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“…Goblet cells can be observed either isolated [8,12,13] or gathered in clusters [10,14] and show different topographical distribution across the conjunctival surface [15,16] .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, different cytoplasmic features can be detected: dark and tightly arranged granules, clear and loosely arranged granules, mucus-discharging cells or with an empty theca [9,12,17] .…”

Section: Introductionmentioning

confidence: 99%

“…The above-mentioned morphological variability could be influenced by gender and hormonal conditions [12] or external stimuli [18][19][20] . Also several pathological conditions, such as keratoconjunctivitis sicca [16,[21][22][23][24] , vernal conjunctivitis [25,26] or intolerance to contact lens [19,27,28] , are able to induce structural changes of conjunctival epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Tear Substitutes on Conjunctival Epithelium of Mice

Aragona

Micali

Paladino³

et al. 2007

Ophthalmic Res

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Aim: The effects of the administration of three tear substitutes on normal conjunctival epithelium of the mouse, with particular regard to goblet cells, were studied. Methods: Three-month-old Swiss CD 1 mice were divided into four groups of 7 animals each. Group 1 was untreated (control). The other animals were treated with the instillation of 5 drops/day for 10 days as follows: group 2, 0.2% sodium hyaluronate (SH); group 3, 0.5% hydroxypropylmethylcellulose (HPMC); group 4, 0.9% NaCl. The entire conjunctiva was processed for light and electron microscopy. A morphometric study was performed to evaluate: the number and size of goblet cells and the number, size and optical density of mucin granules. Furthermore, Alcian blue/periodic acid-Schiff (AB/PAS) staining for goblet cells was carried out. Results: The SH-treated group showed that the area and number of goblet cells were similar to the untreated group; the mucin granules were reduced in number and optical density but increased in size. The HPMC-treated group showed a reduced area of goblet cells with reduced optical density of mucin granules. The saline-treated group showed that goblet cells were reduced in area and number; mucin granules showed a reduction in number and optical density and an increased size. The AB/PAS stain showed that each treatment was able to induce a treatment-specific modification of the staining characteristics of goblet cells. Conclusion: Our results strongly suggest that tear substitutes can induce treatment-specific modifications of the conjunctival epithelium with particular regard to goblet cell structure and ultrastructure.

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Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?

et al. 2009

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Although odorant-binding proteins (OBP) are one of the most abundant classes of proteins in the mammalian olfactory mucus, they have only recently been ascribed a functional role in the detection of odorants by olfactory neurons. Among the three OBPs described in the rat, OBP-1f is mainly secreted by the lateral nasal glands (LNG) and Bowman's glands, and its expression is transcriptionally regulated by food deprivation in the olfactory mucosa, but not in LNG. Therefore, mucus composition might be locally regulated by hormones or molecules relevant to nutritional status. Our aim has been to investigate the mechanisms of such physiological regulation at the cellular level, through both the examination of OBP-1f synthesis sites in the olfactory mucosa and their putative regulation by leptin, a locally acting satiety hormone. Immunohistochemical observations have allowed the identification of a novel population of OBP-1f-secreting cells displaying morphological and functional characteristics similar to those of epithelial mucous cells. Ultrastructural analyses by both transmission and scanning electron microscopy has enabled a more complete cytoarchitectural characterization of these specialized olfactory mucous cells in their tissue environment. These globular cells are localized in discrete zones of the olfactory epithelium, mainly in the fourth turbinate, and are often scattered from the basal to the apical surface of the epithelium. They contain numerous small droplets of mucosubstances. Using an in-vitro-derived model of olfactory mucosa primary culture, we have been able to demonstrate that leptin increases the production of mucus by these cells, so that they constitute potential targets for the physiological modulation of mucus composition by nutritional cues.

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Morphological and Morphometric Analysis on the Rabbit Conjunctival Goblet Cells in Different Hormonal Conditions

Cited by 19 publications

References 28 publications

The Acinar and Ductal Organisation of the Tarsal Accessory Lacrimal Gland of Wolfring in Rabbit Eyelid

The Acinar and Ductal Organisation of the Tarsal Accessory Lacrimal Gland of Wolfring in Rabbit Eyelid

Effects of Tear Substitutes on Conjunctival Epithelium of Mice

Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?

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