Morphological and molecular identification of Parabronema skrjabini of camels (Camelus dromedary) in Najaf province

A-Fatlawy, Haider H.; Al-Fatlawi, Monyer A.

doi:10.33899/ijvs.2020.127101.1459

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…A total of 50 milk samples were obtained from camels randomly collected from different areas around Nineveh Governorate between May and June 2019. The samples were collected directly from the udder as following: first streams were discarded, and the subsequent milk was placed in sterile tubes and stored in the cool box (12) and transported to the Department of Veterinary Public Health, College of Veterinary Medicine, Public Health Laboratory. All milk samples were inoculated on blood agar and Mannitol salt agar 7.5% plates 118 g/L.…”

Section: Camel Milk Samplesmentioning

confidence: 99%

Direct detection of Staphylococcus aureus in camel milk in the Nineveh governorate by using the PCR technique

Sheet¹,

Jwher²,

Al-Sanjary³

et al. 2021

IJVS

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Staphylococcus (S.) aureus is the main facultative organism of contagious intramammary infections from lactating animals. It is considered a major foodborne organism that can cause food poisoning conditions around the world. Camels are very important to the lifestyle of many countries because they can produce milk that contains the major components such as proteins, energy, vitamins, and minerals. The present study used a polymerase chain reaction (PCR) method on a base of the nuc gene as a target gene, which is a specific gene that recognizes the S. aureus amongst other microorganisms. Fifty milk samples have been collected from camels from different areas of the Nineveh Governorate, Iraq. According to the phenotypic characteristics, isolation and identification of S. aureus have been accomplished by characterizing the shape of the colonies, painting the suspected isolates by gram stain, using the biochemical tests such as coagulase and catalase. In this study, S. aureus was isolated from 70% (35/50) camel milk samples. The classical method of identifying the S. aureus isolated from camel milk was consistent with the PCR method. The PCR technique indicated that all positive S. aureus possessed the nuc gene. The increased percentage of S. aureus isolated from the camel milk has a relationship with the type of farm management, poor nutrition, and/or environmental conditions, rather than treatment of the infected camel. The PCR method is considered one of the best-used techniques to identify the S. aureus isolated from camel milk by detection of nuc gene, the specific gene of S. aureus.

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Section: Camel Milk Samplesmentioning

confidence: 99%

Direct detection of Staphylococcus aureus in camel milk in the Nineveh governorate by using the PCR technique

Sheet¹,

Jwher²,

Al-Sanjary³

et al. 2021

IJVS

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“…It can also transmit babesiosis, theileriosis, and rickettsiasis (21). The findings revealed evolutionary links of the locally identified isolates to different world isolates, probably due to the trademoving of food animals (22).…”

Section: Discussionmentioning

confidence: 86%

Genotypic analysis of ticks species infesting cattle in Al-Diwaniyah abattoir

Ali¹,

Atiyah²,

Al-Fatlawi³

et al. 2021

IJVS

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Different vectors are considered critical for disease transmission between animals; however, ticks play a significant role in the dissemination of various infectious illnesses of animals and human importance. The current work was carried out to categorize ticks genetically of those isolated from cattle that entered Al-Diwaniyah abattoir. In the present study, 50 tick samples were collected and subjected to microscopic examination and genetic-based methods of polymerase chain reaction and partial gene sequencing, both utilized the mitochondrial cytochrome c oxidase subunit I (COX1) gene as a genotyping element. The findings of the microscopic examination showed that the ticks were from Hyalomma spp. Further analysis, the polymerase chain reaction revealed the genus of Hyalomma of the ticks, but when the PGS was performed, one sample of H. detritum, three samples of H. excavatum, and two samples of H. marginatum were identified. When the phylogenetic analyses were conducted, H. detritum showed close genetic similarity to an isolate from Spain EU827695.1. H. excavatum revealed similarity with isolates from India MK863382.1 and Turkey MT230050.1. In contrast, H. marginatum displayed close identity to an isolate from Iran (MG557555.1). In conclusion, these findings may indicate evolutionary links of the locally identified isolates to different world isolates, probably due to the trade-moving of animals.

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“…Consequently, it is unlikely to diagnose this pathogen according to the clinical or postmortem examination (25). Consequently, definitive diagnosis of this disease is based on a significant rise in antibody and molecular detection (25)(26)(27). The decrease in pregnancy and/or milk production might be attributed to the high susceptibility of C burnetii to the placenta, amniotic fluids, and udder (8,18).…”

Section: Discussionmentioning

confidence: 99%

First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq

Al-Graibawi¹,

Yousif²,

Gharban³

et al. 2021

IJVS

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This study aims to detect Coxiella burnetii in one-humped female camels (Camelus dromedarius) using ELISA and confirmation of infection by PCR with the phylogenetic analysis of local isolates. The 91 adult female camels were selected for clinical examination and blood sampling from different areas in Badra and Al-Numaniyah districts in Wasit governorate, Iraq, from February to April 2019. The prevalence of Coxiella (C.) burnetii was 19.8% and 4.4% by ELISA and PCR, respectively. Targeting 16S rRNA genes from three positive samples were documented in the Genbank-NCBI under accession numbers of MN900579.1, MN900580.1, and MN900581.1. Clinical evaluation revealed insignificant variation in temperature, pulse, respiratory rates, and lymph node enlargement among the positive and negative animals. The findings also showed that camels of the Badra regions have positive signs. burnetii compared to other regions, and the infection was increased significantly in April and March. In conclusion, our findings confirmed the prevalence of C. burneth among Iraqi female camels, suggesting that these animals might be a source of the pathogen for humans and other animal species. Therefore, further studies are necessary to provide more detailed data about the prevalence of C. burnetiito to improve effective control measures.

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Morphological and molecular identification of Parabronema skrjabini of camels (Camelus dromedary) in Najaf province

Cited by 4 publications

References 14 publications

Direct detection of Staphylococcus aureus in camel milk in the Nineveh governorate by using the PCR technique

Direct detection of Staphylococcus aureus in camel milk in the Nineveh governorate by using the PCR technique

Genotypic analysis of ticks species infesting cattle in Al-Diwaniyah abattoir

First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq

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