Morphological and functional features of the blood in humans during a nine-day stay in an oxyargon environment with various oxygen contents

Ivanova, Saška; Моруков, Б. В.; Максимов, Г. В.; Чурин, А. А.; Yarlykova, Yu. V.; Labetskaya, O. I.

doi:10.1134/s0362119712070092

Cited by 4 publications

(2 citation statements)

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“…The morphology of erythrocytes is sensitive to the action of sodium nitroprusside and phenolic compounds of natural origin. Considering the abundant literature [55–57] and the results of our previous study of the lipid component of erythrocyte membranes [58, 59], we thought that the structure and condition of erythrocytes is interconnected not only with the lipid components of membranes, but also with proteins of the cytoskeleton. Therefore, in the second series of experiments, we quantified the condition of the erythrocyte membrane-skeletal proteins during hypoxia and treatment with nitroprusside sodium and quercetin.…”

Section: Resultsmentioning

confidence: 99%

Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane-Skeletal Proteins, and Erythrocyte Structure under Hypoxia

Revin

Gromova

Revina

et al. 2019

BioMed Research International

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This study shows that membrane-associated cytoskeletal protein structures and the erythrocyte morphology undergo profound changes during hypoxia. Hypoxia also intensified oxidative processes in the lipid phase of the bilayer of red blood cell membranes. Sodium nitroprusside impaired the morphology of red blood cells and altered quantitative and qualitative composition of membrane-skeletal proteins. The findings suggest that hypoxia causes changes at all levels of red blood cell organization, which can cause the functional disorders of hemoglobin oxygen-transporting properties and, eventually, the complete degradation of red blood cells. The use of flavonoids has a protective effect against hypoxia.

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Section: Resultsmentioning

confidence: 99%

Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane-Skeletal Proteins, and Erythrocyte Structure under Hypoxia

Revin

Gromova

Revina

et al. 2019

BioMed Research International

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“…Many studies [1][2][3], including ours [4][5][6], have provided evidence that the lipid phase plays an active role in the regulation of the oxygen-transport function of haemoglobin during hypoxia, which is the cause and consequence of many cardiovascular diseases [7]. However, the human body often faces a situation when in some tissues and, in particular, in the blood, there may be an excessive amount of oxygen.…”

Section: Introductionmentioning

confidence: 99%

The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

Ревин

Gromova

Revina

et al. 2018

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes' oxygentransport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen-air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl 2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca 2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids' composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

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Surface-enhanced Raman spectroscopy in modern chemical analysis: advances and prospects

Eremina¹,

Semenova²,

Sergeeva³

et al. 2018

Russ. Chem. Rev.

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The review concerns the state of the art, the advances in and prospects for application of surface-enhanced Raman spectroscopy (SERS) in chemical analysis. Key advantages (nondestructive origin, high sensitivity and selectivity, easiness of sample preparation) and drawbacks (a relatively small number of analytes, insufficient metrological characteristics of certain objects) of the method are pointed out. Particular attention is paid to the development of novel chemical approaches and to the design of versatile optical sensors to significantly enhance the analytical potential of the method and to extend the range of analytes. Examples are given of the application of SERS in key branches of science and technology that require both qualitative and quantitative chemical analysis of complex objects, viz., environmental monitoring, fuel and energy area, medical diagnosis, biology and biochemistry. The bibliography includes 502 references.

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Morphological and functional features of the blood in humans during a nine-day stay in an oxyargon environment with various oxygen contents

Cited by 4 publications

References 0 publications

Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane-Skeletal Proteins, and Erythrocyte Structure under Hypoxia

Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane-Skeletal Proteins, and Erythrocyte Structure under Hypoxia

The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

Surface-enhanced Raman spectroscopy in modern chemical analysis: advances and prospects

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