Morphological and biochemical responses of sainfoin (Onobrychis viciifolia Scop.) ecotypes to salinity

Abstract

This study was conducted to evaluate the effect of different NaCl concentrations (0, 100 and 150 mM) on the morphological and biochemical parameters of different sainfoin ecotypes ‘Koçaº’, ‘Malya,’ ‘Altýnova’ and ‘Ulaþ’ under controlled conditions. The morphological parameters included seed germination, seedling and root lengths, and seedling fresh and dry weights while the biochemical parameters included chlorophyll and proline contents, lipid peroxidation (MDA), and the activities of antioxidant enzymes (SOD, CAT, APX, and GR). The results demonstrated that increase in NaCl concentration caused an overall decrease in morphological parameters and an increase in the biochemical parameters in all ecotypes. The findings showed that among all ecotypes, ‘Koçaþ’ had higher chlorophyll and proline content, increased activity of CAT, GR, and APX, and increased fresh and dry weight in response to salt stress. To the best of our knowledge, this is the first report on the evaluation of enzymatic and non-enzymatic defense systems in sainfoin plants under salt stress.

Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture

“…Irani et al (2015) reported that proline accumulation was elevated in leaves of sainfoin that were exposed to drought stress under field conditions. Proline accumulation was seen in the sainfoin leaves against stress stimulation (Beyaz et al, 2017). Pýwowarczyk et al (2014) also reported that the accumulation of proline was significantly greater under PEG-6000 induced osmotic stress than under unstressed conditions in vitro in a Lathyrus sativus (grass pea) culture.…”

“…In contrast, Irani et al (2015) reported that strong negative association between antioxidant activity and total chlorophyll in the -1 (µmol min -1 (µmol min -1 (U min -1 (µmol min -1 (µmol min -1 mg -1 FW) mg -1 FW) mg -1 FW) mg -1 FW) mg -1 FW) mg -1 FW) mg -1 FW) sainfoin leaves under drought stress. Additionally, Beyaz et al (2017) reported that the chlorophyll contents of the leaves of the sainfoin seedlings increased under salt stress. Here, it may be speculated that different stressors may be effective on chlorophyll contents in different ways.…”

Exogenous antioxidants on quality of cabbage seeds

“…ROS at low concentrations play a vital role in cell signaling, which supports and makes germination viable, in addition to having a protective effect against pathogens. However, in excess, ROS accumulation impairs germination due to oxidative damage in proteins, lipids and deoxyribonucleic acid (Awasthi et al 2017;Kumar et al 2015), which, inevitably, promotes the activation of the vegetal defense system, synthetizing antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), all responsible for ROS elimination or reduction (Xiong et al 2018;Beyaz et al 2017).…”

“…Superoxide dismutase (SOD) is the first enzyme that acts on the defense mechanism against oxidative stress and breaks free oxygen into oxygen peroxide (H 2 O 2 ), followed by the coordinated action of a set of enzymes including catalase (CAT) and ascorbate peroxidase (APX), which convert H 2 O 2 into water and oxygen (Beyaz et al 2017;Barreiros et al 2006).…”

The complete chloroplast genome sequence of Sainfoin (Onobrychis viciifolia)

“…Sainfoin can be grown in salty and arid regions, and chloroplast is one of the primary organelles affected by salt and drought stress (Beyaz et al 2018;Beyaz 2019aBeyaz , 2019bBeyaz and Yildiz 2020). The chloroplast is an important organelle that has unique genome sequence, and the chloroplast genome of plants has been a hot spot in plant molecular evolution and systematics (Clegg et al 1994).…”