Moringa Oleifera Lam. Leaves Extract Reduces Human T-Cell Hyporesponsiveness and Dna Damage Induced by Oxidative Stress

Abstract

Chronic inflammatory conditions lead to the generation of excessive reactive oxygen species which can give rise to T cell hyporesponsiveness. Thus there has been an interest to target these mediators with nutrients which have anti-oxidant properties. Accordingly Moringa oleifera extract has been reported to have anti-inflammatory and anti-oxidant properties. We have now examined the effects of Moringa oleifera Lam. on the depressed human T-cell responses, (IL-2 and IL-2 mRNA production) and DNA damage (quantifying the oxidized derivative of deoxyguanosine, 8-OHdG) induced oxidative stress. T-cells treated with oxidative substances, uric acid, aluminum chloride, hydrogen peroxide, and ultraviolet irradiation showed marked reduction in IL-2 production, which was prevented by pre-treating the cells with Moringa extract. These effects correlated with its prevention of the down-regulation of IL2 gene expression and a reduction in the DNA damage caused by oxidative stress. Thus the ability of Moringa extract to reduce inflammation is likely to relate to its ability to prevent T-cell hyporesponsiveness experienced in inflammatory diseases, through an action at the pre-transcriptional level.

A review of Leaves and Seeds Moringa oleifera Extract: The potential Moringa oleifera as Antibacterial, Anti-Inflammatory, Antidiarrhoeal, And Antiulcer Approaches To Bacterial Gastroenteritis

“…Some of the phytochemical contents of M. oleifera were found to be different in each part of the plant, as shown in Table 2 [11], [22], [23], [24], [25], [26], [27], [28], [29]. Table 3 shows the qualitative phytochemical screening result of both aqueous and ethanol extract of M. oliefera leaves [28].…”

“…It is also important to note that the phytochemical constituent has a role in determining the antibacterial activities of the leaf extract [17], [18]. In another study using qualitative and quantitative phytochemical analysis, M. oleifera leaf extract also contains tannins, saponins, phenols, alkaloids, and phlobatannins [26].…”

“…In another study, it was found that M. oleifera can eliminate the production of monocyte-derived macrophage factors, such as TNFα, IL-6, and IL-8. M. oleifera exerts some of its immunerelated effects mainly through direct pathogen removal or modulating the balance of pro-and anti-inflammatory mediators released from various types of immune cells by regulating the activity of signaling pathways, such as the NF-B pathway (Figure 1) [25], [26], [27].…”

Moringa oleifera Lam and its Therapeutic Effects in Immune Disorders

“…However, few studies have focused on the response of immunocytes to MO. Kooltheat et al found that MO could eliminate the production of monocyte-derived macrophage factors, like tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-8 (Kooltheat et al, 2014(Kooltheat et al, , 2017. Notably, the decrease in these factors was evident at both mRNA and protein levels.…”