Monolithic hydrogel nanowells-in-microwells enabling simultaneous single cell secretion and phenotype analysis

Abstract

We used laser micropatterning to fabricate monolithic hydrogel nanowells in standard microwell plates for simultaneous single cell secretion and phenotype analysis.

Field-Portable Leukocyte Classification Device Based on Lens-Free Shadow Imaging Technique

“…Recently, several point-of-care (POC) systems have been proposed to assess blood count with high precision to overcome these drawbacks. Microfluidics-based blood analysis systems that either perform fluorescence analysis or combine magnetic purification of cells with chemotaxis assay from small blood samples have been reported [ 13 , 14 , 15 , 16 , 17 ]. However, they all have accessibility limitations and require additional fluorescence analysis to identify the class and concentration of fluorescently stained cells or additional fluorescence staining to confirm the pathway of cell migration.…”

Multiplexed analysis of signalling proteins at the single-immune cell level

“…However, droplet microfluidics is hampered by the inability to replace reagents which can affect the detection of secreted proteins with high sensitivity. Open nano wells have been utilized for the purpose of highly sensitive detection of singlecell secreted proteins 38,39 . The shortcoming with this technology is that the secreted proteins are not contained in the well but can diffuse out of them 38 .…”

“…Open nano wells have been utilized for the purpose of highly sensitive detection of singlecell secreted proteins 38,39 . The shortcoming with this technology is that the secreted proteins are not contained in the well but can diffuse out of them 38 . In contrast, microchambers have been developed for isolation of single cells with the flexibility of conventional 96-well plates 28,29 .…”

A photo-triggering double cross-linked adhesive, antibacterial, and biocompatible hydrogel for wound healing

“…Hydrogels have attracted increasing attention in recent years for their unique properties such as high-water content, viscoelastic, and biocompatibility ( Gong et al., 2013 ; Guo et al., 2013 ; Wei et al., 2021 ). The porous structure and suitable swelling ratio support various biomedical applications such as biosensing ( Choi et al., 2020 ; Mao et al., 2021 ), drug delivery ( Zhao et al., 2021 ), tissue engineering ( Ghorbani et al., 2020 ), and microenvironment regulation ( Fan et al., 2014 ). Moreover, numerous surface groups ensure the combination of hydrogels with functional materials to enhance mechanical property and treatment effect ( Kwak et al., 2009 ; Van Nieuwenhove et al., 2016 ; Ahmad et al., 2017 ).…”