Multiplexed analysis of signalling proteins at the single-immune cell level

Dietsche, Claudius; Hirth, Elisabeth; Dittrich, Petra S.

doi:10.1039/d2lc00891b

Cited by 5 publications

(1 citation statement)

References 41 publications

(92 reference statements)

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“…Valve setups can allow studying complex behaviors such as cell polarization by trapping cells in hydrodynamic traps together with barcoded nanobeads to capture selective secreted proteins while also enabling fluidic control, media exchange, and most importantly, cell isolation using pneumatically activated valves. 126 Moreover, valve systems are ideal for downstream sorting and washing steps for antibody sequencing from hydrodynamically trapped cells because pneumatic valves are already implemented within the fluidic chip. 127 Even though valve systems remain niche compared to droplets and wells in antibody discovery, several published applications demonstrate the power that precise fluidic control can exercise to detect and manipulate antibodyproducing cells.…”

Section: Valve-based Techniquesmentioning

confidence: 99%

Antibodies, repertoires and microdevices in antibody discovery and characterization

Schlotheuber,

Lüchtefeld,

Eyer

2024

Lab Chip

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Section: Valve-based Techniquesmentioning

confidence: 99%

Antibodies, repertoires and microdevices in antibody discovery and characterization

Schlotheuber,

Lüchtefeld,

Eyer

2024

Lab Chip

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Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review

Azuaje-Hualde,

Alonso-Cabrera,

de Pancorbo

et al. 2024

Anal Bioanal Chem

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Monitoring cell secretion in complex microenvironments is crucial for understanding cellular behavior and advancing physiological and pathological research. While traditional cell culture methods, including organoids and spheroids, provide valuable models, real-time monitoring of cell secretion of signaling molecules remains challenging. Integrating advanced monitoring technologies into these systems often disrupts the delicate balance of the microenvironment, making it difficult to achieve sensitivity and specificity. This review explored recent strategies for integrating the monitoring of cell secretion of signaling molecules, crucial for understanding and replicating cell microenvironments, within cell culture platforms, addressing challenges such as non-adherent cell models and the focus on single-cell methodologies. We highlight advancements in biosensors, microfluidics, and three-dimensional culture methods, and discuss their potential to enhance real-time, multiplexed cell monitoring. By examining the advantages, limitations, and future prospects of these technologies, we aim to contribute to the development of integrated systems that facilitate comprehensive cell monitoring, ultimately advancing biological research and pharmaceutical development.

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Application and prospect of microfluidic devices for rapid assay of cell activities in the tumor microenvironment

Zhu,

Cui,

Jiang

et al. 2024

Biomicrofluidics

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The global impact of cancer on human health has raised significant concern. In this context, the tumor microenvironment (TME) plays a pivotal role in the tumorigenesis and malignant progression. In order to enhance the accuracy and efficacy of therapeutic outcomes, there is an imminent requirement for in vitro models that can accurately replicate the intricate characteristics and constituents of TME. Microfluidic devices exhibit notable advantages in investigating the progression and treatment of tumors and have the potential to become a novel methodology for evaluating immune cell activities in TME and assist clinicians in assessing the prognosis of patients. In addition, it shows great advantages compared to traditional cell experiments. Therefore, the review first outlines the applications and advantages of microfluidic chips in facilitating tumor cell culture, constructing TME and investigating immune cell activities. Second, the roles of microfluidic devices in the analysis of circulating tumor cells, tumor prognosis, and drug screening have also been mentioned. Moreover, a forward-looking perspective is discussed, anticipating the widespread clinical adoption of microfluidic devices in the future.

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Multiplexed analysis of signalling proteins at the single-immune cell level

Abstract: High numbers of tumour-associated macrophages (TAMs) in the tumour microenvironment are associated with a poor prognosis. However, the effect of TAMs on tumour progression depends on the proteins secreted by...

Cited by 5 publications

References 41 publications

Antibodies, repertoires and microdevices in antibody discovery and characterization

Antibodies, repertoires and microdevices in antibody discovery and characterization

Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review

Application and prospect of microfluidic devices for rapid assay of cell activities in the tumor microenvironment

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