Monodisperse Branched Molybdenum‐Based Bioactive Nanoparticles Significantly Promote Osteogenic Differentiation of Adipose‐Derived Stem Cells

Abstract

Adipose‐derived stem cells (ADSCs) are considered to be ideal stem cell sources for bone‐tissue regeneration owing to their ease of collection and high activity. However, the regulation of osteogenic differentiation of ADSCs using biomaterials without adding growth factors is still not satisfactory. For the first time, molybdenum‐doped bioactive glass nanoparticles with a radial porous morphology (Mo‐rBGNs) are reported and their role in the osteogenic differentiation of ADSCs is investigated. The results show that Mo‐rBGNs exhibit radially porous and spherical morphology, relatively homogeneous particle size (200–400 nm), and excellent apatite‐forming bioactivity. They do not affect the proliferation of ADSCs, but significantly regulate their osteogenic differentiation and biomineralization. 5% Mo‐rBGNs significantly enhance the alkaline phosphatase activity and biomineralization ability and promote the osteogenic gene expressions of collagen I secretion and bone sialo protein in ADSCs. A reasonable and promising strategy for designing nanoscale bioactive materials with the excellent osteogenic ability for stem cell–based bone tissue regeneration is provided.

Monodispersed β‐Glycerophosphate‐Decorated Bioactive Glass Nanoparticles Reinforce Osteogenic Differentiation of Adipose Stem Cells and Bone Regeneration In Vivo

“…The enhanced osteogenic differentiation of ADMSCs was probably attributed to the bioactive feature of BGNs and GP. Previous studies showed that monodispersed BGNs could induce the osteogenic differentiation of ADMSCs through the signaling pathway of TGF‐beta 14,33. The main reasons of GP functionalization enhancing the osteogenic differentiation of BGN on ADMSCs could be shown as follows.…”

“…Previous studies have shown that monodispersed bioactive glass nanoparticles (BGNs) presented enhanced cell uptake and biodegradation, and improved biominerialization ability, demonstrated promising biomedical applications in bioimaging, gene delivery, bone regeneration, and wound healing 10,21,25,27,30. Monodispersed BGNs and nanoscale silicon‐based biomaterials could also efficiently enhance the osteogenic differentiation of osteoblasts and bone marrow stem cells 31–33. Recent years, our group further found that monodispersed BGNs could induce the osteogenic differentiation of adipose mesenchymal stem cells (ADMSCs) through activating the TGF‐beta signaling pathway, and the molybdenum‐based branched BGNs could also promote the osteogenic differentiation of ADMSCs 34,35.…”

3D Printing and Nanotechnology: A Multiscale Alliance in Personalized Medicine

“…BGNs compared with traditional bioactive glass have a controlled surface area, which improves the local formation of apatite and osteogenic bioactivity. [ 101,212 ] Chen et al. (2020) decided to use poly(citrate‐siloxane) (PCS) to coat the surface of BGNS (BGNS@PCS) to produce photoluminescent biomimetic elastomeric BGNS with potential application in bioimaging.…”

Fine analysis of interaction mechanism of bioactive glass surface after soaking in SBF solution: AFM and ICP-OES investigations

“…In addition, recent studies show the possibility of further benefit brought by these materials. They may have other advantages such as the functionalization of their surface for the transport of molecules [9] of biological interest and activates biological reactions useful for the restoration of bone tissue such as cell differentiation [10], osteogenesis and angiogenesis [11]. All these characteristics must be investigated during the engineering of new biomaterials.…”