Tumor de mama é a neoplasia mais freqüente em cadelas, entretanto, há controvérsias sobre os fatores que influenciam o desenvolvimento do tumor. O objetivo deste trabalho foi estudar o perfil das cadelas com tumor de mama atendidas no Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul (HCV-UFRGS). Foram coletados os dados de 85 cadelas apresentando neoplasias mamárias, entre junho de 1999 e maio de 2000. Foram analisados o tempo de evolução dos tumores, neoplasias anteriores, histórico reprodutivo, localização, tamanho dos nódulos, ulcerações, metástases pulmonares e resultados da histopatologia. A partir destes dados, 71,8% foram lesões malignas e, 28,2%, benignas. A maioria dos malignos foi de carcinomas e, dos benignos, adenoma. A idade média foi de 9 anos para as cadelas com tumores benignos e de 9 anos para os malignos. O progestágeno foi associado a um maior número de tumores benignos. As pseudocieses foram relacionadas a tumores de mama malignos. Tanto o uso de progestágenos como as pseudocieses foram relacionados com o aparecimento precoce de neoplasias mamárias em cadelas.

3D printing and nanotechnology have been two important tools in the development of therapeutic approaches for personalized medicine. More recently, their alliance has been improved in an effort to build innovative, versatile, multifunctional, and/or smart medical and pharmaceutical products. Therefore, an extensive review about scientific studies that ally 3D printing and nanomaterials in the development of new approaches for pharmaceutical and medical applications for the treatment and prevention of diseases is presented here. The articles are classified into five categories according to their main application: Cell growth and tissue engineering, antimicrobial, drug delivery, stimulus‐response, and theranostics. Semisolid extrusion, inorganic nanoparticles, and cell growth and tissue engineering are the most reported 3D printing technique, type of nanomaterial, and application, respectively. The increase in papers dedicated to these areas is also notable, especially in the 2019 and 2020, when semisolid extrusion became the most used technique, overcoming fused deposition modelling. In fact, this review highlights that the possibility of an alliance between 3D printing and nanotechnology for the production of multiscale materials is undoubtedly a great opportunity for knowledge and innovation in the pharmaceutical and medical area.

3D printing has been widely used for the personalization of therapies and on-demand production of complex pharmaceutical forms. Recently, 3D printing has been explored as a tool for the development of topical dosage forms and wound dressings. Thus, this review aims to present advances related to the use of 3D printing for the development of pharmaceutical and biomedical products for topical skin applications, covering plain dressing and products for the delivery of active ingredients to the skin. Based on the data acquired, the important growth in the number of publications over the last years confirms its interest. The semisolid extrusion technique has been the most reported one, probably because it allows the use of a broad range of polymers, creating the most diverse therapeutic approaches. 3D printing has been an excellent field for customizing dressings, according to individual needs. Studies discussed here imply the use of metals, nanoparticles, drugs, natural compounds and proteins and peptides for the treatment of wound healing, acne, pain relief, and anti-wrinkle, among others. The confluence of 3D printing and topical applications has undeniable advantages, and we would like to encourage the research groups to explore this field to improve the patient’s life quality, adherence and treatment efficacy.

The interaction of small molecules with DNA has been quite important, since this biomolecule is currently the major target for a wide range of drugs in clinical use or advanced clinical research phase. Thus, the present work aimed to assess the interaction process between the bioactive compound 11a-N-tosyl-5-carba-pterocarpan, (LQB-223), that presents antitumor activity, with DNA, employing spectroscopic techniques, electrophoresis, viscosity and theoretical studies. Through UV-vis and molecular fluorescence spectroscopy, it was possible to infer that the preferential quenching mechanism was static, characterized by non-fluorescent supramolecular complex formation between the LQB-223 and DNA. The binding constant was 1.94∙10Lmol (30°C) and, according to the thermodynamic parameters, the main forces involved in the interaction process are hydrophobic. Potassium iodide assay, competition with ethidium bromide, fluorescence contact energy transfer and melting temperature profile of DNA were employed to evaluate the binding mode. Except for KI assay, all results obtained indicated minor groove as the preferential binding mode of LQB-223 to DNA. These observations were supported by ionic strength assay, viscosity and molecular dynamics and docking studies. Finally, electrophoresis analysis demonstrated that the interaction does not promote DNA fragmentation, but it leads to variation in the migration profile after increasing the ligand concentration.

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O carcinoma inflamatório mamário é um carcinoma anaplásico com características clínicas e histopatológicas como crescimento rápido, envolvimento difuso, eritema, calor e dor nas mamas, edema nos membros posteriores, extensa infiltração de células inflamatórias, células epiteliais malignas nos linfonodos regionais apresentando um péssimo prognóstico. O cão é a única espécie animal em que esta neoplasia ocorre espontaneamente, entretanto apresenta uma incidência bastante rara tanto em humanos quanto em caninos. Este trabalho descreve dois casos de carcinoma inflamatório mamário em duas cadelas da raça Dobermann apresentando aumento de volume mamário com espessamento da pele na região das mamas, com secreção liquido-sanguinolenta local, aumento da temperatura e dor no local e edema de membros posteriores. Em ambos os casos, o resultado do exame citológico revelou carcinoma anaplásico e os animais apresentaram uma rápida evolução da doença em menos de 30 dias, optando-se pela eutanásia nos dois casos. Os achados de necrópsia indicaram metástases pulmonares em ambos os cães e com o exame histológico confirmaram o diagnóstico de carcinoma anaplásico. Este trabalho elucida bem os sinais clínicos característicos dos cães com carcinoma inflamatório, que se assemelham muito a mastite, demonstra o prognóstico reservado desta enfermidade, além de chamar a atenção para este raro tumor ter ocorrido na mesma raça.Descritores: Carcinoma inflamatório mamário, tumor de mama, câncer.

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Descreve-se, no Rio Grande do Sul, Brasil, o primeiro caso de adenomatose pulmonar ("jaagsiekte ") em um ovino da raça Karakul, fêmea de 2 anos, filha de pais importados da Alemanha. O ovino morreu de acidose lática por sobrecarga alimentar e as lesões pulmonares representaram achados incidentais. Macroscopicamente encontraram-se duas áreas esbranquiçadas nodulares com aproximadamente 4 e 6cm de diâmetro respectivamente, com bordos irregulares que, microscopicamente, correspondiam a tumoração epitelial bem diferenciada, formando projeçôes papilares para o interior do lúmen alveolar e bronquiolar. Não foram encontrados indícios de associação com Maedi/Visna através dos achados sorológicos e histopatológicos. O diagnóstico de adenomatose pulmonar, baseou-se na histopatologia.