Monoclonal Hybridoma Antibodies against Human IgE and Their Use in a Rapid and Sensitive Enzyme Immunoassay for the Semiquantitative Assessment of Total IgE Levels in Human Blood

Chandler, Howard M.; Coulter, A.R.; Healey, K.; Kornitschuk, M; MacGregor, A.; Hurrell, John G.R.

doi:10.1159/000234879

Cited by 18 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Normally, the IgE quantity in serum is ~5x10 -5 mg/ml. In 1983, the anti-human IgE monoclonal antibody was produced by Chandler et al (13), and since then the total IgE levels can be detected by the method of semiquantitative ELISA. In the present study, the positive rate of total IgE was 65.0% by the method of immunoblotting, which may result from the selection of cases or certain hypersensitivity that were not IgE-mediated (14).…”

Section: Patients With Grade 3 Sige ---------------------------------mentioning

confidence: 99%

Analysis of total immunoglobulin E and specific immunoglobulin E of 3,721 patients with allergic disease

et al. 2015

View full text Add to dashboard Cite

Abstract. Due to the increase in the prevalence and incidence of allergic diseases, improving the sensitivity and specificity of screening indexes is critical. Total immunoglobulin E (IgE) is a traditional index for judging allergic diseases, while its specificity is relatively poor. Serum-specific IgE (sIgE) is an objective index with high specificity in the diagnosis of allergic diseases. In the present research, the total IgE and sIgE of 3,721 patients with allergic diseases were analyzed to further illuminate the association between them. The data were derived from 3,721 patients. The serum-sIgE to 14 types of common allergens and total IgE were detected. A total of 2,419 cases (65.0%) of 3,721 patients exhibited increasing total IgE and 1,215 patients (32.7%) exhibited positive sIgE. The consistency rate of the two indexes was 60.4%, and the κ-value was 0.28. In 135 patients with normal total IgE, 82.2% exhibited one sIgE positive and 17.8% exhibited two or more sIgE positive. While the number of positive sIgE increased and the detecting level enhanced, the number of positive total IgE markedly increased. Patients (84.1%) with increasing total IgE were associated with positive sIgE, but the increase of total IgE could not be completely explained by the total accumulation of sIgE. Total IgE may play an important role on screening allergic disease while sIgE could be used as crucial evidence for allergy diagnosis. Although the consistency of the two methods was poor, neither total IgE nor sIgE could replace each other. Combining the two indexes with clinical manifestations together will improve the method.

show abstract

Section: Patients With Grade 3 Sige ---------------------------------mentioning

confidence: 99%

Analysis of total immunoglobulin E and specific immunoglobulin E of 3,721 patients with allergic disease

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Whilst the terms "RAST" and "RAST class" are still widely used in clinical practice to refer to the presence of allergen-specific IgE in serum, the original radioallergosorbent test has been made obsolete by advances in assay technology. Modern immunoassays for the detection of allergen-specific IgE have undergone relevant improvements, including substitution of the radio-labelled polyclonal antibodies with enzyme-labelled monoclonal detection antibodies [13], the development of solid phase systems with higher allergen binding capacities [14], and the introduction of fully automated devices [15]. Most of the currently used IgE detection systems are calibrated against the World Health Organization (WHO) Standard 72/502, and can be considered "quantitative" assays [12].…”

Section: Assays For the In Vitro Determination Of Specific Ige Antibomentioning

confidence: 99%

Specific IgE Testing (RAST)

Crameri¹

2009

Aspergillosis: From Diagnosis to Prevention

View full text Add to dashboard Cite

The diagnosis of allergic diseases is based on a combination of clinical history, physical examination, in vivo challenges, and in vitro test methods for the detection of allergen-specific IgE antibodies in the serum of patients. In spite of the fact that the determination of allergen-specific IgE in serum is widely used in daily clinical practice, one should be aware that such determinations, although useful for routine screening of possible allergies, are not sufficient to diagnose the disease. Clinical history and provocation tests should always be considered to confirm an allergy indicated by elevated allergen-specific IgE levels in serum. This specially applies to the diagnosis of mould allergy in general, and of Aspergillus fumigatusrelated allergic complications in particular.

show abstract

“…Several monoclonal an tibodies specific for human IgE have been produced [1,11,12], but no assay system utilizing such mono clonal antibodies for PBMC culture supernatants has been described and validated. A monoclonal anti-IgE antibody has been produced which can be used in conjunction with a highly specific polyclonal antiIgE antibody for assay of culture supernatants, as well as being suitable for use as l25I-labelled tracer in conventional solid phase assays of lower sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A Monoclonal Antibody-Based Radioimmunoassay for the in vitro Production of IgE by Lymphocyte Cultures

Zaunders

Ziegler

Penny

et al. 1985

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Very sensitive radioimmunoassay systems have been described for the measurement of IgE produced in cultures of human peripheral blood mononuclear cells. However, differing results have been reported when cultures from non-atopic donors are stimulated with pokeweed mitogen, which may be due to cross-reactivity of anti-IgE antibodies with IgG. A monoclonal antibody specific for the Fc region of human IgE, and two polyclonal affinity-purified antibodies to IgE were tested for binding to ¹²⁵I-labelled IgE myeloma proteins and polyclonal IgG in a sensitive double antibody precipitation assay. The monoclonal antibody and one of the polyclonal antibodies bound only IgE, whereas the other polyclonal antibody bound a significant proportion of labelled IgG. A solid phase radioimmunoassay was developed which combined the specificity of the monoclonal antibody with the sensitivity of the first polyclonal antibody as radioactive tracer. A second assay system was also tested using the cross-reacting antibody as tracer. Supernatants of pokeweed mitogen-stimulated peripheral blood mononuclear cell cultures from non-atopic donors were examined for IgE synthesis using both assays. The assay based on the monoclonal antibody did not detect IgE synthesis, whilst the second assay, based on the cross-reacting antibody indicated that spurious IgE had been produced in the same cultures. This study shows that protein-binding assays provide a simple means for checking the specificity of antibodies in solid phase radioimmunoassays, and confirms that pokeweed mitogen does not stimulate IgE production by cells from non-atopic donors when measured by a specific radioimmunoassay.

show abstract

Monoclonal Hybridoma Antibodies against Human IgE and Their Use in a Rapid and Sensitive Enzyme Immunoassay for the Semiquantitative Assessment of Total IgE Levels in Human Blood

Cited by 18 publications

References 14 publications

Analysis of total immunoglobulin E and specific immunoglobulin E of 3,721 patients with allergic disease

Analysis of total immunoglobulin E and specific immunoglobulin E of 3,721 patients with allergic disease

Specific IgE Testing (RAST)

A Monoclonal Antibody-Based Radioimmunoassay for the in vitro Production of IgE by Lymphocyte Cultures

Contact Info

Product

Resources

About