A Monoclonal Antibody-Based Radioimmunoassay for the in vitro Production of IgE by Lymphocyte Cultures

Zaunders, John; Ziegler, John B.; Penny, Ronald; Cooper, David A.

doi:10.1159/000233853

Cited by 7 publications

(3 citation statements)

References 11 publications

(12 reference statements)

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“…The plasma levels of IgG and lgM were measured by standard nephelometric method using a Beckman Nephelometer. Levels of the immunoglobulins were determined in unconcentrated lavage fluid using a radioimmunoassay method similar to that described previously [11]. ELF IgG and lgM levels were calculated as described above for albumin.…”

Section: Evaluation Of Elf Volumes and Albumin Contentmentioning

confidence: 99%

Changes in epithelial lining fluid albumin associated with smoking and interstitial lung disease

Roberts

Cairns²,

Bryant

et al. 1993

Eur Respir J

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Albumin is an important plasma protein which is useful in the assessment of in vivo membrane permeability in the lung. In subjects with interstitial lung disease (ILD) the relationship between albumin recovered from bronchoalveolar lavage (BAL) and other markers of inflammatory activity may provide useful information of the pathogenesis of the disease process. Unfortunately, its measurement is hampered by the variable dilution in BAL fluid. In this study, urea was used as a marker of epithelial lining fluid (ELF) dilution allowing the calculation of an apparent epithelial lining fluid volume and adjusted albumin content. We examined the relationship between ELF albumin content and BAL cell counts, immunoglobulin content, respiratory function tests and gallium lung scans in both smokers and nonsmokers with and without interstitial lung disease. Forty seven subjects with connective tissue disease and interstitial lung disease and 51 subjects with either connective tissue disease but no pulmonary involvement or non pulmonary malignancy (18 current smokers) underwent BAL, gallium lung scans and respiratory function tests. The subjects with ILD were further subdivided into those with active ILD or bronchiolitis using cluster analysis. In smokers without ILD an increased ELF volume and a decrease in ELF albumin were found. Increased ELF albumin was related to increased age. In subjects with ILD, increased albumin was strongly correlated with increased BAL lymphocyte absolute and differential counts, which overwhelmed any age or smoking effect. These findings suggest a possible causal relationship between lung vascular permeability and lymphocyte numbers in subjects with interstitial lung disease and reinforce the need to consider smoking and age as confounding factors in BAL analysis.

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Section: Evaluation Of Elf Volumes and Albumin Contentmentioning

confidence: 99%

Changes in epithelial lining fluid albumin associated with smoking and interstitial lung disease

Roberts

Cairns²,

Bryant

et al. 1993

Eur Respir J

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“…The plasma level s of IgG and lgM were measured by standard nephelometric methods using a Beckman Nephelometer. Levels of the immunoglobulins were determined in unconcentrated lavage fluid using a radio-immunoassay as described previously [10,11]. ELF JgG and lgM were calculated as described above for albumin.…”

Section: Analysis Of Elf Volume and Albumin Contentmentioning

confidence: 99%

Smoking-induced changes in epithelial lining fluid volume, cell density and protein

et al. 1992

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Bronchoalveolar lavage has proved a useful research technique for recovering cellular and molecular contents of the lower respiratory tract. Because the recovered fluid is variably diluted, an accurate estimation of molecular and cellular concentrations can only be made if the epithelial lining fluid volume recovered is also known. It has been suggested that smoking may alter epithelial lining fluid volume by reducing clearance or by stimulating production and, thus, affect the interpretation of bronchoalveolar lavage studies. In this study, urea was used as an endogenous marker of epithelial lining fluid volume in a comparison of 26 smokers and 31 nonsmokers. The mean epithelial lining fluid volume recovered from smokers was significantly greater than that of nonsmokers (2.4 +/- 1.40 ml vs 1.2 +/- 0.75 ml, p less than 0.005). The total cellular concentration in the bronchoalveolar lavage fluid in smokers was also greater (94.2 +/- 46 x 10(6) vs 33.9 +/- 21.5 x 10(6) cells per 300 ml lavage), even when corrected for bronchoalveolar lavage volume recovered (63.1 +/- 32.5 x 10(6) vs 24.9 +/- 13.3 x 10(6) cells per 100 ml recovered lavage fluid). This was true for macrophage, lymphocyte and neutrophil cell numbers. However, when corrected for the apparent epithelial lining fluid volume, only the macrophage count remained significantly higher in the smokers compared with nonsmokers (30.66 +/- 20.7 x 10(6) vs 18.21 +/- 8.6 x 10(6) macrophages.ml-1 ELF). In addition, concentrations of albumin and immunoglobulin M (IgM) were significantly lower in smokers after correction for epithelial lining fluid volume. These results highlight smoking as a confounding factor in the interpretation of bronchoalveolar lavage data.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…This is a solid-phase RIA which was adapted from an IgE assay developed by Zaunders et al [30]. Briefly, flexible polyvin ylchloride microtitre plates (Dynatech Laboratories, Va.) were coated with specific antichain IgG (Dako, USA; Cat No.…”

Section: Radio-immunoassaymentioning

confidence: 99%

Effect of Interferon on the in vitro Synthesis of Paraprotein by Plasma Cells in Myeloma

McGinnes¹,

Penny²

1988

Acta Haematol

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The aim of this study was to determine the effects of interferon on in vitro immunoglobulin synthesis by the plasma cells of patients with multiple myeloma. Bone marrow cultures were set up in the presence of media alone or α-interferon. Supernatants were harvested over a 24-hour period and assayed for paraprotein immunoglobulin synthesis using radio-immunoassays developed in this department. The 24-hour paraprotein synthesis ranged from 9 to 493 μg/10⁶ plasma cells in patients with IgG myeloma (n = 11), and from 0.3 to 8.3 μg/10⁶ plasma cells in those with IgA (n = 4). α-Interferon had variable effects on paraprotein synthesis in IgG myelomas, causing an inhibition of synthesis (up to 61%) in 3 patients and an enhancement of synthesis (up to 95%) in 5 cases. In IgA myelomas, on the other hand, α-interferon caused inhibition of synthesis in 1 case. In the 2 patients studied during α-interferon administration, IgG synthesis was significantly reduced. More cases are currently being investigated. Clearly, immunoglobulin synthesis by plasma cells shows variable modulation by α-interferon in vitro.

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A Monoclonal Antibody-Based Radioimmunoassay for the in vitro Production of IgE by Lymphocyte Cultures

Cited by 7 publications

References 11 publications

Changes in epithelial lining fluid albumin associated with smoking and interstitial lung disease

Changes in epithelial lining fluid albumin associated with smoking and interstitial lung disease

Smoking-induced changes in epithelial lining fluid volume, cell density and protein

Effect of Interferon on the in vitro Synthesis of Paraprotein by Plasma Cells in Myeloma

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