Monoclonal antibody for identification of Bacteroides gingivalis lipopolysaccharide

Millar, Stephen J.; Chen, P B; Hausmann, Ernest

doi:10.1128/jcm.25.12.2437-2439.1987

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…Results from a representative experiment for five individual spleens are presented as mean stimulation index (E/C signifies mean counts per minute of stimulated cultures/ mean counts per minute of unstimulated cultures) ± standard deviation. Serum samples were tested for antibody to B. gingivalis by immunoblotting on nitrocellulose with a Bio-Dot microfiltration apparatus (Bio-Rad) as previously described (18) by using 4-chloro-1-naphthol (Bio-Rad) as the substrate or by enzyme-linked immunosorbent assay (ELISA) as previously described (2) with p-nitrophenylphosphate (Sigma) as the substrate. Mouse sera were diluted 1/500.…”

Section: 5mentioning

confidence: 99%

Protective immunization against experimental Bacteroides (Porphyromonas) gingivalis infection

et al. 1990

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The effects of immunization in modulating the pathogenesis of Bacteroides (Porphyromonas) gingivalis infection in a murine model system were examined. BALB/c mice were immunized by intraperitoneal injection with B. gingivalis ATCC 53977 (one injection per week for 3 weeks), or with a lithium diiodosalicylate (LIS) extract (one injection per week for 3 weeks), or with lipopolysaccharide (LPS; one intravenous or intraperitoneal injection) from this same strain. Two weeks after the final immunization, the mice were challenged by * Corresponding author.

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Section: 5mentioning

confidence: 99%

Protective immunization against experimental Bacteroides (Porphyromonas) gingivalis infection

et al. 1990

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“…Confirmation is done with an indirect fluorescent-antibody (IFA) test using polyclonal rabbit antiserum (10). Monoclonal antibodies (MAb) have proven valuable in identifying other organisms by various methods (3,4,11,12). It was thought that this approach would prove to be a quick and useful tool for identifying M. bovis when used with an IFA test.…”

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confidence: 99%