1987
DOI: 10.1128/jcm.25.12.2437-2439.1987
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Monoclonal antibody for identification of Bacteroides gingivalis lipopolysaccharide

Abstract: A monoclonal antibody, BBG-25, raised in BALB/c mice demonstrated specificity for Bacteroides gingivalis lipopolysaccharide. Immunoblotting indicated that this monoclonal antibody does not cross-react with lipopolysaccharide prepared from enterobacterial organisms or from other Bacteroides species.

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Cited by 6 publications
(2 citation statements)
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“…Results from a representative experiment for five individual spleens are presented as mean stimulation index (E/C signifies mean counts per minute of stimulated cultures/ mean counts per minute of unstimulated cultures) ± standard deviation. Serum samples were tested for antibody to B. gingivalis by immunoblotting on nitrocellulose with a Bio-Dot microfiltration apparatus (Bio-Rad) as previously described (18) by using 4-chloro-1-naphthol (Bio-Rad) as the substrate or by enzyme-linked immunosorbent assay (ELISA) as previously described (2) with p-nitrophenylphosphate (Sigma) as the substrate. Mouse sera were diluted 1/500.…”
Section: 5mentioning
confidence: 99%
“…Results from a representative experiment for five individual spleens are presented as mean stimulation index (E/C signifies mean counts per minute of stimulated cultures/ mean counts per minute of unstimulated cultures) ± standard deviation. Serum samples were tested for antibody to B. gingivalis by immunoblotting on nitrocellulose with a Bio-Dot microfiltration apparatus (Bio-Rad) as previously described (18) by using 4-chloro-1-naphthol (Bio-Rad) as the substrate or by enzyme-linked immunosorbent assay (ELISA) as previously described (2) with p-nitrophenylphosphate (Sigma) as the substrate. Mouse sera were diluted 1/500.…”
Section: 5mentioning
confidence: 99%
“…Confirmation is done with an indirect fluorescent-antibody (IFA) test using polyclonal rabbit antiserum (10). Monoclonal antibodies (MAb) have proven valuable in identifying other organisms by various methods (3,4,11,12). It was thought that this approach would prove to be a quick and useful tool for identifying M. bovis when used with an IFA test.…”
mentioning
confidence: 99%