Monoclonal antibodies against bacteria

Macario, Alberto J. L.; Macario, Everly Conway de

doi:10.1016/0734-9750(88)90001-8

Cited by 5 publications

(2 citation statements)

References 106 publications

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“…4 One of the major reasons for the lack of suitable drug candidates is that there is practically no academic discovery pipeline in the United States because of insufficient funding. 9 Novel approaches to treat infectious diseases, for instance, the discovery and up-scaling of antibodies against bacterial pathogens, 11,12 and identifying novel antibiotics through genomic mining 13,14 are still in their infancy.…”

Section: ■ Introductionmentioning

confidence: 99%

Pyrazolyl Thioureas and Carbothioamides with an NNSN Motif against MSSA and MRSA

et al. 2021

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A novel series of copper-activatable drugs intended for use against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillinresistant S. aureus (MRSA) were synthesized, characterized, and tested against the MSSA strain Newman and the MRSA Lac strain (a USA300 strain), respectively. These drugs feature an NNSN structural motif, which enables the binding of copper. In the absence of copper, no activity against MSSA and MRSA at realistic drug concentrations was observed. Although none of the novel drug candidates exhibits a stereocenter, sub-micromolar activities against SA Newman and micromolar activities against SA Lac were observed in the presence, but not in the absence, of bioavailable copper.Copper influx is a component of cellular response to bacterial infections, which is often described as nutritional immunity.

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Section: ■ Introductionmentioning

confidence: 99%

Pyrazolyl Thioureas and Carbothioamides with an NNSN Motif against MSSA and MRSA

et al. 2021

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“…However, polyclonal antibodies (PAbs) against plant pathogens produced by animal immunization may show cross-reactivity with unrelated pathogenic species due to the limited specificity of PAbs. 8 With the development of monoclonal antibodies (MAbs), specificity was improved since they target a single epitope in a pathogen protein. 9 Hence, various antibodybased diagnostic methods such as enzyme-linked immunosorbent assays (ELISAs) 10,11 , immunoblot 12 , immunofluorescent test 13 and lateral flow devices (LFD) 14 have been developed and widely used to identify plant pathogens.…”

Section: Antibody-based Detectionmentioning

confidence: 99%

Development of point-of-care and multiplex diagnostic methods for the detection of plant pathogens

Lau¹

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New diagnostic technologies for the detection of plant pathogens with high multiplexing ability and point-of-care (POC) capability are an essential tool in the fight to reduce the large agricultural production losses caused by plant diseases. The main desirable characteristics for such diagnostic assays are high specificity, sensitivity, reproducibility, quickness, cost efficiency and highthroughput multiplex detection capability. The aim of this project is to develop new methods combining nucleic acid amplification, multiplexing and point-of-care application for the detection of plant pathogens.Isothermal amplification methods, such as recombinase polymerase amplification (RPA) which operates at low constant temperature are especially suited for POC applications. However, the development of a rapid and simplified detection method for the isothermal amplification products in resource-poor settings is still challenging. A new method to visualize the success of the amplification step, and therefore the presence of pathogen DNA in a sample, was developed based on bridging flocculation. This method requires minimal equipment and can be assessed by the naked eye allowing its use in POC settings. The method was initially applied to the rapid and sensitive detection of several important plant pathogens and subsequently extended to animal and human pathogens to demonstrate the wide applications of the approach. A nanoparticle based electrochemical biosensor was also developed for rapid and sensitive detection of amplified plant pathogen DNA on screen-printed carbon electrodes. Gold nanoparticles were employed as a probe for electrochemical assessment by differential pulse voltammetry (DPV). This method was 10,000 times more sensitive than conventional polymerase chain reaction (PCR)/gel electrophoresis and could readily identify P. syringae infected plant samples even before the disease symptoms were visible.To allow multiplex detection of plant pathogen, a novel method that can screen for thousands of plant pathogens with high specificity and sensitivity using molecular inversion probes (MIPs) was been developed. As proof of concept, a MIP targeting a unique DNA sequence present in the F.oxysporum f.sp. conglutinans (Foc) genome was designed. The specificity, sensitivity and detection limit of the assay were assessed and used to detect the presence of pathogen in infected Arabidopsis thaliana samples. This methodology successfully detected as little as 2.5 ng of pathogen DNA and it was highly specific, being able to accurately differentiate Fusarium oxysporum f.sp. conglutinans from other fungal pathogens such as Botrytis cinerea and even pathogens of the same species such as Fusarium oxysporum f.sp. lycopersici. Finally, a diagnostic platform for POC plant pathogen detection using a combination of surface-enhanced Raman ii scattering (SERS) and RPA was developed for rapid multiplex detection. The RPA-SERS method was faster, more sensitive than polymerase chain reaction and could detect as little as 2 copies of...

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Melt Spinning of Poly(3‐hydroxybutyrate) for Tissue Engineering Using Electron‐Beam‐Irradiated Poly(3‐hydroxybutyrate) as Nucleation Agent

Vogel

Voigt

Tändler

et al. 2008

Macromolecular Bioscience

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Electron-beam-irradiated poly(3-hydroxybutyrate) was used as a nucleating agent for poly(3-hydroxybutyrate) in a melt-spinning process. Molecular data and thermal properties of the irradiated samples were determined. The thermal properties of the nucleated melts were determined to assess the influence of the nucleation agents, and then spinning tests were carried out. Thermal and textile properties of the spun fibers were also determined. Estimations of the improvement of the crystallization in the spinline and of the inhibition of secondary crystallization in the fibers from the use of the described blood-compatible nucleation agents are given.

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Monoclonal antibodies against bacteria

Cited by 5 publications

References 106 publications

Pyrazolyl Thioureas and Carbothioamides with an NNSN Motif against MSSA and MRSA

Pyrazolyl Thioureas and Carbothioamides with an NNSN Motif against MSSA and MRSA

Development of point-of-care and multiplex diagnostic methods for the detection of plant pathogens

Melt Spinning of Poly(3‐hydroxybutyrate) for Tissue Engineering Using Electron‐Beam‐Irradiated Poly(3‐hydroxybutyrate) as Nucleation Agent

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