Protective immunization against experimental Bacteroides (Porphyromonas) gingivalis infection

Chen, P B; Davern, Lynda B.; Schifferle, Robert E.; Zambon, Joseph J.

doi:10.1128/iai.58.10.3394-3400.1990

Cited by 66 publications

(58 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, immunization with highly purified P. gingivalis fimbriae was found to be protective against periodontal bone destruction in P. gingivalis-infected gnotobiotic rats (8). On the other hand, Chen et al (3) have reported that immunization with P. gingivalis lipopolysaccharide does not result in readily detectable antibody levels nor does it afford protection from a challenge infection with P. gingivalis.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the immunodominant antigens of Porphyromonas gingivalis 381 in high‐responder patients

Boutsi

Koseki

Nishihara

et al. 1996

Oral Microbiology and Immunology

View full text Add to dashboard Cite

The immunodominant antigens of Porphyromonas gingivalis 381 whole cells that reacted with sera from high-responder patients were examined in this study. Whole cells, phenol-water extracted lipopolysaccharide, and fimbriae from P. gingivalis 381 were analyzed using sera from 14 patients with adult periodontitis, rapidly progressive periodontitis or juvenile periodontitis as well as from two healthy subjects. Western blot analysis and enzyme-linked immunosorbent assay (ELISA) were performed. On Western blots, among many prominent protein bands, a smear was observed which was removed after adsorption of the sera with P. gingivalis phenol-water extracted lipopolysaccharide. Two major protein bands of 43 kDa and 41 kDa were found to be prominent even at very high dilutions of sera, the latter of which showed the same molecular weight as the fimbrilin band. These two bands were resistant to treatment by papain and trypsin. ELISA titers remained high after adsorption of the sera with P. gingivalis phenol-water extracted lipopolysaccharide. The results of this study suggest that the 43-kDa and the fimbrilin (41 kDa) proteins may play an important role as immunodominant antigens of P. gingivalis 381.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the immunodominant antigens of Porphyromonas gingivalis 381 in high‐responder patients

Boutsi

Koseki

Nishihara

et al. 1996

Oral Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…Serum samples were collected at death and tested for antibody activity to P. gingivalis or A. actinomycetemcomitans by ELISA, as previously described (10). Briefly, 96-well microtiter plates were coated with P. gingivalis or A. actinomycetemcomitans at a cell concentration of OD5go=0.3 (~2xlOVml).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…Serum antibody reactivity to P. gingivalis and A. actinomycetemcomitans was also evaluated by Western immunoblot analysis, as previously described (10). After separation of a P. gingivalis lithium diiodosalicylate extract (10) and a sonicate extract of A. actinomycetemcomitans by sodium dodecyl sulfatepolyacrylamide (12%) gel electrophoresis, the antigens were transferred to nitrocellulose using a semi-dry transfer system (Polyblot transfer apparatus.…”

Section: Western Immunobiot Analysismentioning

confidence: 99%

“…The spleens from individual mice were removed, weighed and single cell suspensions prepared (10). Total leukocyte counts for each spleen were determined using a Coulter Counter (Coulter, Hialeah, FL).…”

Section: Lymphoproliferative Responsesmentioning

confidence: 99%

“…actinomycetemcomitans 75 cells were assessed, as previously described (10). Spleen cell cultures were incubated at 37°C for 48 h in 5% CO,, 95% air.…”

Section: Lymphoproliferative Responsesmentioning

confidence: 99%

See 2 more Smart Citations

Host responses induced by co‐infection with Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in a murine model

Chen

Davern

Katz

et al. 1996

Oral Microbiology and Immunology

View full text Add to dashboard Cite

In this study, evidence is presented that mixed infection with the periodontal pathogens Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans results in a synergistic effect in their pathogenicity and in their ability to induce humoral and cellular host responses. BALB/c mice were injected subcutaneously on the back with P. gingivalis ATCC 53977, A. actinomycetemocomitans 75 or a mixture of both bacteria. Samples of blood and fluid from abscesses formed at the site of injection (first degree) or distant from the injection site were collected for microbiologic analysis. Serum and spleens were obtained for evaluation of humoral and cellular responses to P. gingivalis and A actinomycetemocomitans. Mice injected with A. actinomycetemcomitans had first-degree lesions only, whereas mice injected with P. gingivalis and A. actinomycetemcomitans had lesions at first- and second-degree sites from which both bacterial species were isolated. A serum anti-P. gingivalis response was induced in P. gingivalis-injected mice, which was higher in mice injected with P. gingivalis and A. actinomycetemcomitans. This pattern was not seen in the anti-A, actinomycetemcomitans response. Lymphoproliferative responses to phytohemagglutinin, Escherichia coli lipopolysaccharide and P. gingivalis of spleen cells from infected mice were decreased, especially following co-infection. Furthermore, co-infection of mice resulted in the greatest decrease in the number of CD5+, especially CD4+ lymphocytes.

show abstract

Host modulation of tissue destruction caused by periodontopathogens: effects on a mixed microbial infection composed ofPorphyromonas gingivalisandFusobacterium nucleatum

Ebersole

Feuille

Kesavalu

et al. 1997

Microbial Pathogenesis

View full text Add to dashboard Cite

Protective immunization against experimental Bacteroides (Porphyromonas) gingivalis infection

Cited by 66 publications

References 34 publications

Characterization of the immunodominant antigens of Porphyromonas gingivalis 381 in high‐responder patients

Characterization of the immunodominant antigens of Porphyromonas gingivalis 381 in high‐responder patients

Host responses induced by co‐infection with Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in a murine model

Host modulation of tissue destruction caused by periodontopathogens: effects on a mixed microbial infection composed ofPorphyromonas gingivalisandFusobacterium nucleatum

Contact Info

Product

Resources

About