Monoclonal antibodies for the identification of New World Leishmania species

Species Typing in Dermal Leishmaniasis

“…Several authors have described the use of monoclonal antibodies to identify Leishmania species of the Leishmania (Viannia) (92)(93)(94)(95) or Leishmania (Leishmania) (96, 97) subgenus, or both (62,(98)(99)(100). These antibodies to some extent specifically recognize cultured promastigotes of different species, or they are genus specific.…”

Recent advances in the taxonomy of the New World leishmanial parasites

“…Moreover, analyses employing mAbs speci®c for selected species complexes of Leishmania showed antigenic diversity (level of expressed antigenic determinants) occurring among Leishmania strain variants, but no qualitative or quantitative reactivity pattern could be consistently related to the parasite origin or geographic area of isolation [1,13,26]. The pathogenic implications of these data remain to be elucidated.…”

Transmembrane Molecules for Phylogenetic Analyses of Pathogenic Protists: Leishmania -Specific Informative Sites in Hydrophilic Loops of Trans- Endoplasmic Reticulum N -Acetylglucosamine-1-Phosphate Transferase

“…live extracellularly in the digestive tracts of blood-sucking female sand flies of various species as their vectors and intracellularly in the macrophages of different mammalian hosts, i.e., human, canine, rodent, and other reservoir animals. The complexities of such unusual ecological niches undoubtedly contribute to Leishmania speciation.A large body of biological, biochemical, immunological, and molecular data (7,10,23,55) exists in the literature suggesting that the genus Leishmania consists of three groups (55) as follows: (i) subgenus Leishmania, which includes species complexes distributed in both the New World and the Old World, e.g., L. majorturanica-L. gerbilli; (ii) subgenus Viannia, whose members are restricted to the Neotropics, e.g., L. braziliensis [Leishmania (Viannia) braziliensis] and L. panamensis; and (iii) several unclassified species (3), e.g., L. enriettii. The pathogenic species listed above have long been subjected to diagnostic typing and phylogenetic analyses (15,23,49).…”

“…A large body of biological, biochemical, immunological, and molecular data (7,10,23,55) exists in the literature suggesting that the genus Leishmania consists of three groups (55) as follows: (i) subgenus Leishmania, which includes species complexes distributed in both the New World and the Old World, e.g., L. major…”

“…The pathogenic species listed above have long been subjected to diagnostic typing and phylogenetic analyses (15,23,49). They are referred to as species complexes due to subspecies heterogeneity, as shown in some population genetic analyses (26,29,51).…”