2007
DOI: 10.1128/ec.00282-06
|View full text |Cite
|
Sign up to set email alerts
|

Transmembrane Molecules for Phylogenetic Analyses of Pathogenic Protists: Leishmania -Specific Informative Sites in Hydrophilic Loops of Trans- Endoplasmic Reticulum N -Acetylglucosamine-1-Phosphate Transferase

Abstract: A sequence database was created for the Leishmania N-acetylglucosamine-1-phosphate transferase (nagt) gene from 193 independent isolates. PCR products of this single-copy gene were analyzed for restriction fragment length polymorphism based on seven nagt sequences initially available. We subsequently sequenced 77 samples and found 19 new variants (genotypes). Alignment of all 26 nagt sequences is gap free, except for a single codon addition or deletion. Phylogenetic analyses of the sequences allow grouping the… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

3
50
0
1

Year Published

2008
2008
2021
2021

Publication Types

Select...
5
2
2

Relationship

1
8

Authors

Journals

citations
Cited by 55 publications
(54 citation statements)
references
References 69 publications
3
50
0
1
Order By: Relevance
“…The sequences of nuclear protein-coding genes were similarly analyzed in two additional studies. Dozens of isolates from China together with several hundred from elsewhere were examined in one study for five different single-copy genes (42). All canine and human VL isolates fell into the same clade of the L. infantum/L.…”
Section: Leishmania Species Complexitymentioning
confidence: 99%
“…The sequences of nuclear protein-coding genes were similarly analyzed in two additional studies. Dozens of isolates from China together with several hundred from elsewhere were examined in one study for five different single-copy genes (42). All canine and human VL isolates fell into the same clade of the L. infantum/L.…”
Section: Leishmania Species Complexitymentioning
confidence: 99%
“…used for the present study include L. amazonensis (LV 78 clone 12-1), L. major (RM), L. tropica (K27) and L. infantum (Adana #7) (see http://66.99.255.20/cms/micro/sample%20list.pdf and Waki et al, 2007 for the origin of the strains used).…”
Section: Leishmania Cultivation and Transfectionmentioning
confidence: 99%
“…Of these eight enzymes, only the last one, i. e. ferrochelatase (Fe-C) has been shown to exist functionally by nutritional studies (Chang et al, 1975) and enzyme assays (Salzman et al, 1982) in different trypanosomatid protozoa. Most recently, we have found the putative Fe-C sequences of high homology in all Leishmania isolates grouped phylogenetically into some 20 genotypes, including most Old World species, i. e. L. infantum /donovani, L. major, L. tropica, L. turanica and L. gerbilli (Waki et al, 2007). Sequence conservation of Fe-C suggests that it may function beyond heme biosynthesis, since protoporphyrin (PROTO) is cytotoxic and thus would not be widely available to serve as a substrate of this enzyme to produce the heme needed by Leishmania.…”
Section: Introductionmentioning
confidence: 99%
“…So, there are also many DNA-based technique applied to phylogenetic study of Leishmania. The most common method is sequencing or RFLP analysis of the specific gene or gene fragment, such as mini-exon gene (Fernandes et al 1994), ribosomal internal transcribed spacer (El Tai et al 2001;Kuhls et al 2005), kinetoplast DNA maxicircle divergent region (Flegontov et al 2006), gp63 (Mauricio et al 2007;Waki et al 2007), genes encoding DNA polymerase catalytic subunit, RNA polymerase II largest subunit (Croan et al 1997) etc. However, phylogenetic analysis focusing on only particular sequences in the genome could be misleading.…”
Section: Introductionmentioning
confidence: 99%