Mono- and Poly-unsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells

Tanguy, Emeline; Bagneaux, Pierre Costé de; Kassas, Nawal; Ammar, Mohamed-Raafet; Wang, Qili; Haeberlé, Anne-Marie; Raherindratsara, Juliette; Fouillen, Laëtitia; Renard, Pierre; Montero-Hadjadje, Maïté; Chasserot‐Golaz, Sylvette; Ory, Stéphane; Gasman, Stéphane; Bader, Marie‐France; Vitale, Nicolas

doi:10.1016/j.celrep.2020.108026

Cited by 24 publications

(28 citation statements)

References 72 publications

(117 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chromaffin cells were washed with Locke's solution (without ascorbic acid) and processed for catecholamine release measurements by amperometry as previously described [36,37]. A carbon fiber electrode of 5 μm diameter (ALA Scientific Instruments) was held at a potential of +650 mV compared with the reference electrode (Ag/AgCl) and approached close to one cell.…”

Section: Carbon Fiber Amperometrymentioning

confidence: 99%

Somatostatin analogue pasireotide (SOM230) inhibits catecholamine secretion in human pheochromocytoma cells

et al. 2022

Self Cite

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Carbon Fiber Amperometrymentioning

confidence: 99%

Somatostatin analogue pasireotide (SOM230) inhibits catecholamine secretion in human pheochromocytoma cells

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…A 结构变化以及宿主 -病原体相互作用机理 . 由于 Phos-tag 能够特异性地结合到磷酸单酯基团上, Iwama 等人 [166] 不饱和脂质是生物体内重要的分子, 对细胞的多种功能至关重要 [213,214] , 与癌症、心血管疾病、糖尿病等密切相关 [215][216][217][218][219] . 在 DESI-MSI 分析中, 应用于不饱和脂质的其它衍生化试剂较少 , 主要是 AgNO3.…”

Section: Maldi-msi 分析中的成功应用能够对脂质 a 进行高效的可视化成像研究有助于深入理解感染期间脂质unclassified

Chemical derivatization: recent advances in mass spectrometry imaging

Li¹,

Qin²,

Jiang³

et al. 2022

Sci. Sin.-Chim

View full text Add to dashboard Cite

Recently, mass spectrometry imaging (MSI) has been drawing more and more attention due to its continuous development and improvement as well as its vital role in the study of biological science. Many MSI-based strategies have been widely applied to the in situ qualitative and quantitative detection and imaging of endogenous/exogenous molecules in tissues. Among, matrix assisted laser desorption/ionization MSI (MALDI-MSI) and desorption electrospray ionization MSI (DESI-MSI) are generally regarded as two the most commonly used molecular imaging techniques. Although MSI, as a histology-based emerging molecular imaging technique, has significant advantages over other imaging techniques because it is a label-free technique which provides high sensitivity, high throughput, and molecular specificity derived from the use of mass spectrometers as detectors for a wide variety of ionized biomolecules in situ within a tissue section, it still has obvious shortcomings in the in situ analysis of low-polarity and neutral biomolecules. To solve this issue, the application of chemical derivatization technology in MSI is one of the most important approaches to overcome these deficiencies of MS molecular imaging. This review provides an overview of various chemical derivatization techniques, such as, esterification derivatization, acylation derivatization, addition reaction derivatization, substitution derivatization, oxidative derivatization, and other derivatizations, used in MS analysis, highlights the application of these chemical derivatization methods in the in situ detection and imaging of biomolecules in tissues by MALDI-MSI and DESI-MSI. It can be predicted that with the advance progress of chemical derivatization technology, the application scope of MSI will be further expanded in the fields of spatial metabolomics, spatial lipidomics, spatial proteomics, and spatial glycomics.

show abstract

“…Human tumor cells from freshly resected pheochromocytoma were washed with Locke's solution (140 mM NaCl, 4.7 mM KCl, 2.5 mM CaCl2, 1.2 mM KH2PO4, 1.2 mM MgSO4, 11 mM glucose, 0.01 mM EDTA and 15 mM HEPES, pH 7.5) and processed for catecholamine release measurements by amperometry as previously described (Houy et al, 2015;Tanguy et al, 2020). A carbon fiber electrode of 5 μm diameter (ALA Scientific Instruments) was held at a potential of +650 mV compared with the reference electrode (Ag/AgCl) and approached close to one cell.…”

Section: Carbon Fiber Amperometrymentioning

confidence: 99%

Dysfunction of exocytosis causes catecholamine hypersecretion in patient with pheochromocytoma

Houy

Streit

Drissa

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Pheochromocytoma (Pheo) is a neuroendocrine tumor that develops from chromaffin cells of the adrenal medulla, and is responsible of an excess of catecholamines secretion leading to severe clinical symptoms such as hypertension, elevated stroke risk and various cardiovascular complications. Surprisingly, hypersecretory activity of Pheo has never been explored at the cellular and molecular levels from individual tumor cells. In the present study, we have combined catecholamine secretion measurement by carbon fiber amperometry on human tumor cells directly cultured from freshly resected Pheo, with the analysis by mass spectrometry of the exocytotic proteins differentially expressed between the tumor and the matched adjacent non-tumor tissue. Catecholamine secretion recordings from individual Pheo cells obtained from most patients reveal a higher number of exocytic events per cell associated with faster kinetic parameters. Accordingly, we unravel significant tumor-associated modifications in the expression of key proteins involved in different steps of the calcium-regulated exocytic pathway. Altogether, our findings indicate that dysfunction of the calcium-regulated exocytosis at the level of individual Pheo cell is a cause of the tumor-associated hypersecretion of catecholamines.

show abstract

Mono- and Poly-unsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells

Cited by 24 publications

References 72 publications

Somatostatin analogue pasireotide (SOM230) inhibits catecholamine secretion in human pheochromocytoma cells

Somatostatin analogue pasireotide (SOM230) inhibits catecholamine secretion in human pheochromocytoma cells

Chemical derivatization: recent advances in mass spectrometry imaging

Dysfunction of exocytosis causes catecholamine hypersecretion in patient with pheochromocytoma

Contact Info

Product

Resources

About