Mono- and Diterpenes from Cell Cultures ofThuja occidentalis

Abstract

Cell cultures of THUJA OCCIDENTIALIS L. were found to biosynthesize various mono- and diterpenes when grown on B5-medium. The identification of the constituents was achieved mainly by capillary GLC-MS using fused silica columns and E.I.-mass spectrometry. Monoterpenes of the menthane type were only isolated from the culture medium whereas diterpenes were found in the cell extracts. Thujaplicin derivatives, monoterpenes of an irregular type, were detected in the medium as well as in the cells. Major differences were found between the terpene composition of the cell culture extracts and those from THUJA leaves. The cell cultures accumulated some compounds which are presently unknown as constituents of THUJA plants. On the other hand, the cultures were evidently unable to synthesize the thujone type of monoterpenes.

A Novel Synthesis of 2‐Alkoxy‐3‐hydroxytropones and 2,7‐Dihydroxytropones from Dialkoxy‐8‐oxabicyclo[3.2.1]oct‐6‐en‐3‐ones

“…The former are structural isomers of the natural products MY3-469 [21] and β-thujaplicinol methyl ether. [22] Several hydroxytropones, among them 20a, are reported to exhibit interesting pharmacological properties. [21,23Ϫ25] As for the preparation of α-oxo acetals, it is not unreasonable that optimization of the reaction of dichlorooxabicyclic ketones (e.g.…”

Production of essential oils and flavours in plant cell and tissue cultures. A review

“…Cell suspension cultures of Thuja occidental& L. were found to produce various mono-and diterpenes [24,169]. The diterpenes could only be isolated from the cell extracts, or from the culture medium.…”

Cavity Nest Materials of Northern Flying Squirrels, <em>Glaucomys sabrinus</em>, and North American Red Squirrels, <em>Tamiasciurus hudsonicus</em>, in a Secondary Hardwood Forest of Southern Ontario

“…By using shredded Eastern White Cedar bark as a primary nest material, G. sabrinus and T. hudsonicus also may be limiting exposure to nest-borne ectoparasites. Phytochemical analysis of T. occidentalis bark has revealed 22 volatile compounds, including monoterpenes, fenchene, camphene, camphor, carvacol and paracymene (Shaw 1953;Witte et al 1983;Yatagai et al 1985;Keita et al 2001), several of which have noted antiparasitic or insecticidal properties (Adams et al 1988;Adams 1993;Keita et al 2001).…”