Production of essential oils and flavours in plant cell and tissue cultures. A review

Mulder‐Krieger, Thea; Verpoorte, Robert; Svendsen, A. Baerheim; Scheffer, J. J. C.

doi:10.1007/bf00034451

Cited by 95 publications

(22 citation statements)

References 115 publications

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“…This pattern is frequently observed in enzymological studies of natural product metabolism in cell culture and is believed to be a function of the depletion of some essential nutrient from the culture medium (1,17,44 (Fig. 1), has been described in the intact sage plant (25,26 There are significant differences between the pathway of camphor degradation previously demonstrated in the intact plant and that in cell culture.…”

Section: Monoterpene Biosynthetic Capacity In Culture: In Vitro Measumentioning

confidence: 55%

“…Thus, there are reports of the production of diterpenoid substances in culture at levels exceeding those of the intact plant (42,43) and the induced accumulation of sesquiterpene phytoalexins in culture is well documented (12,13,16), whereas most accounts ofmonoterpene accumulation in cell culture systems (1,17,44) describe either very low levels of production or compositional patterns that differ markedly from those of the intact plant.…”

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confidence: 99%

“…De novo monoterpene biosynthesis, as distinct from monoterpene accumulation (1,17,44) or the biotransformation of exogenous monoterpenes (19), has rarely been directly measured (5). Several investigators (2)(3)(4)34) have demonstrated the conversion of labeled mevalonic acid to more advanced precursors, such as dimethylallyl, geranyl, and farnesyl pyrophosphate, in cell-free extracts from cultures of diverse essential oil species, but it is not possible to determine with certainty whether these metabolites represent intermediates in the biosynthesis of monoterpenes or in the formation of higher products such as phytosterols.…”

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confidence: 99%

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Metabolism of Monoterpenes in Cell Cultures of Common Sage (Salvia officinalis)

Falk¹,

Gershenzon²,

Croteau³

1990

Plant Physiol.

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Leaves of common sage (Salvia officinalis) accumulate monoterpenes in glandular trichomes at levels exceeding 15 milligrams per gram fresh weight at maturity, whereas sage cells in suspension culture did not accumulate detectable levels of monoterpenes (<0.3 nanograms per gram fresh weight) at any stage of the growth cycle, even in the presence of a polystyrene resin trap. Monoterpene biosynthesis from [U-14C]sucrose was also virtually undetectable in this cell culture system. In vitro assay of each of the enzymes required for the sequential conversion of the ubiquitous isoprenoid precursor geranyl pyrophosphate to (+)-camphor (a major monoterpene product of sage) in soluble extracts of the cells revealed the presence of activity sufficient to produce (+)-camphor at a readily detectable level (>0.3 micrograms per gram fresh weight) at the late log phase of growth. Other monoterpene synthetic enzymes were present as well. In vivo measurement of the ability to catabolize (+)-camphor in these cells indicated that degradative capability exceeded biosynthetic capacity by at least 1000-fold. Therefore, the lack of monoterpene accumulation in undifferentiated sage cultures could be attributed to a low level of biosynthetic activity (relative to the intact plant) coupled to a pronounced capacity for monoterpene catabolism.The accumulation of terpenoid natural products in plant cell cultures has been successfully demonstrated in the cases of diterpenoids and sesquiterpenoids, but rarely in the case of monoterpenes. Thus, there are reports of the production of diterpenoid substances in culture at levels exceeding those of the intact plant (42,43) and the induced accumulation of sesquiterpene phytoalexins in culture is well documented (12,13,16), whereas most accounts ofmonoterpene accumulation in cell culture systems (1, 17, 44) describe either very low levels of production or compositional patterns that differ markedly from those of the intact plant.Monoterpenes in intact plants usually accumulate in the extracellular storage spaces of specialized secretory structures, such as glandular trichomes, resin ducts, or resin cavities (35),

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Section: Monoterpene Biosynthetic Capacity In Culture: In Vitro Measumentioning

confidence: 55%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Metabolism of Monoterpenes in Cell Cultures of Common Sage (Salvia officinalis)

Falk¹,

Gershenzon²,

Croteau³

1990

Plant Physiol.

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“…In effect the absorbent is acting as a separate compartment replacing the fully differentiated oil gland in the intact plant. Mulder-Krieger (1988) have also shown that natural compartmentation in Mentha piperita can be induced in cell aggregates. For a two-phase system with in situ product recovery the product must be excreted.…”

Section: In Situ Product Extractionmentioning

confidence: 92%

Overview of Yield Improvement Strategies for Secondary Metabolite Production in Plant Cell Culture

Shuler

1999

Plant Cell and Tissue Culture for the Production of Food Ingredients

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SUMMARY 7Often when a project is initiated plant cell cultures produce low levels of a desired compound. For the project to move to a commercial level, time-dependent volumetric yields (mg.product/L-day) must typically be increased several orders of magnitude. Typically, no single growth enhancing strategy will give such a large increase, but in many cases the simultaneous application of multiple-strategies results in a synergistic interaction and the large increases necessary to bring yields in a plant cell culture process to a useful level. Yield improvement strategies include selection of stable, high-yielding variants; manipUlation of medium components and/or gas phase composition; metabolic manipulation through gene manipulation or use of inhibitors; precursor feeding; use of elicitors; in situ product removal; and use of immobilization or more differentiated or organized plant material. INTRODUCTIONThe commercial application of plant ceIl cultures is likely to be in three areas: (1) facile production of elite plants; (2) as a tool to generate sufficient quantities of metabolites for screening or characterization in a drug discovery program; and (3) for the commercial scale production of a metabolite. In this symposium and book we are focused on the third objective, particularly as it applies to food ingredients. Reviews on food ingredient production and a possible role for plant cell cultures has been published elsewhere

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“…It is now a well-known fact that light as an important physical factor influence the growth and formation of primary and secondary metabolites (Yu et al 2005, Krewzaler and Hahlbrock 1973, Zhong et al 1991, Liu et al 2012, Luczkiewicz et al 2002, Mosaleeyanon et al 2005, Shohael et al 2006, Mulder-Krieger et al 1988. Hence, by optimizing the light conditions, secondary metabolites production can be manipulated effectively and good quality raw material for pharmaceutical industry can be achieved in in vitro cultures of medicinally important plants.…”

Section: Introductionmentioning

confidence: 99%

Sink ? Source System of In vitro Suspension Culture of Celastrus paniculatus under Regulation of Monochromatic Lights

Billore

Khatediya

Jain

2016

Plant Tissue Cult. & Biotech.

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Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug development. The plant cell suspension cultures bear immense potential for production of high-value secondary metabolites and are chosen as alternative sources of raw material for industrial use. In the present study, homogenous cell suspension culture of Celastrus paniculatus a medicinally important plant was established and multifold production of alkaloids and total phenols was obtained under the influence of monochromatic lights. One month old leaf derived friable callus of C. paniculatus was used to raise homogenous suspension culture and kept on rotary shakers in cabinets illuminated with different monochromatic LED lights (Blue, Yellow and Red). The monochromatic lights proved to be a strong abiotic elicitor in driving the production of secondary metabolites so much so that the metabolites were released extracellularly and the medium served as sink or spacious pool for leaked out metabolites from the cell mass. Maximum production and enhancement in alkaloids and phenols (98 and 44.7%, respectively) over control was obtained from cell mass grown under yellow light treatment, followed by blue (64 and 23.7%) and red light (50 and 26%) treatments. Further scale up of secondary metabolite production was hence performed under yellow light conditions, starting from 2.5 gm cell mass suspended in 250 ml of media extended up to 1000 ml culture media for one month. The continuous culture system exhibited remarkable potential of this plant cell system as multifold yield of total alkaloids *Author for correspondence: . 1 Department of Biosciences, Pacific Academy of Higher Education and Research University, Pacific Hills, Udaipur, Rajasthan, India. 176Billore et al.(91.69 μg/1750 ml) and phenols (70.59 μg/1750 ml) was obtained during 30 days of culture under yellow light conditions. The production was remarkably enhanced by 100-folds (5.29 to 48.21 μg; Fig. 2) for alkaloids and 70-folds (1.73 to 46.33 μg; Fig. 3) for phenols, from zero days to 30-day-old culture phase. Hence, strategic implementation of monochromatic lights holds great promises for controlled commercial production of myriads of valuable secondary metabolites from plant cell cultures of important medicinal plants. Present work contributes to the first reports where continuous, enhanced, multifold yield of important secondary metabolites were obtained from cell suspension culture of Celasrus paniculatus an endangered medicinal plant.

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Production of essential oils and flavours in plant cell and tissue cultures. A review

Abstract: The production of essential oils and flavours by plant cell and tissue cultures is reviewed, including the biotransformation of added precursors. Methods which have been employed to increase secondary metabolism are discussed.

Cited by 95 publications

References 115 publications

Metabolism of Monoterpenes in Cell Cultures of Common Sage (Salvia officinalis)

Metabolism of Monoterpenes in Cell Cultures of Common Sage (Salvia officinalis)

Overview of Yield Improvement Strategies for Secondary Metabolite Production in Plant Cell Culture

Sink ? Source System of In vitro Suspension Culture of Celastrus paniculatus under Regulation of Monochromatic Lights

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