Monitoring of the action of drugs in melanoma cells by dynamic laser speckle

González-Peña, Rolando J.; Braga, Roberto A.; Cibrián, Rosa; Palmer, Rosario Salvador; Gil-Benso, Rosario; San-Miguel, Teresa

doi:10.1117/1.jbo.19.5.057008

Cited by 25 publications

(11 citation statements)

References 27 publications

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“…where Z is the threshold parameter, and T j (x, y) is the threshold image at each moment j [22]. The weighted threshold images within a buffer of size n representing the lifetime in the moment j give the final MHI image according to Eq.…”

Section: Theorymentioning

confidence: 99%

Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image

et al. 2016

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This paper reports on the application of the motion history image (MHI) method on dynamic laser speckle processing as a result of a specific drug action on Trypanosoma cruzi parasites. The MHI procedure is based on human action recognition, and unlike other methods which use a sequence consisting of several frames for recognition, this method uses only an MHI per action sequence for recognition. MHI method avoids the complexity as well as the large computation in sequence matching-based methods and detects a change in the speckle pattern. Experimental results of MHI on real-time monitoring of activity (motility) under the influence of the drug demonstrate the effectiveness of the proposed method. The MHI showed an online result without loss of resolution and definition if we compare with routine LASCA method. The obtained results highlight the advantage of the MHI analysis over traditional qualitative image intensity-based methods and demonstrate the potential of measuring the activity of parasites via dynamic laser speckle analysis. The data was further numerically analyzed in the time domain, and the results presented the ability of the technique to monitor the action of the drug, particularly Epirubicin (100 μg/ml).

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Section: Theorymentioning

confidence: 99%

Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image

et al. 2016

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“…For the numerical analysis, active zones of the sample taken as the region of interest were analyzed with respect to their behavior in time by means of the AVD method. 17,18 The AVD computation can be expressed by E Q -T A R G E T ; t e m p : i n t r a l i n k -; e 0 0 7 ; 3 2 6 ; 3 5 8…”

Section: Numerical Evolution Of Absolute Value Of Differencesmentioning

confidence: 99%

Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

et al. 2016

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This paper reports the application of the motion history image (MHI) method for biospeckle processing of a bacterial growth. The method avoids the complexity as well as the large computation in sequence-matching-based methods and detects whether the speckle structure has changed or not. Encouraging experimental results on the real-time evolution of the growing bacteria during 12 h demonstrate the effectiveness of the proposed method. The MHI presented an online result without loss of resolution and definition. In turn, the MHI also presented the ability to provide a close answer to the traditional offline method of generalized differences.

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“…Over time, the successive patterns of biosepckle become susceptible to be tracked, and the changes of biospeckle patterns are associated with biological activity (Braga, 2017;Stoykova et al, 2017). The correlation between the biological activity of a biological materials and variation of the biospeckle pattern over time can be observed in several relevant researches in medicine that studied the monitoring of melanoma (González-Peña et al, 2014) in the evaluation of the activity of bacteria and parasites (Ansari et al, 2016;Grassi et al, 2016) and other variety of applications in agriculture (Zdunek et al, 2014;Retheesh et al, 2016;Sutton and Punja, 2017;Arefi et al, 2018). Szymanska-Chargot et al (2012) and Gao et al (2016) presented the biospeckle laser as a feasible way to detect bruising process in apples and potatoes after mechanical impacts.…”

Section: Introductionmentioning

confidence: 99%

Early prediction of internal bruising in potatoes by biospeckle laser technique

Costa¹,

Braga²,

Boas³

et al. 2018

Afr. J. Agric. Res.

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The internal bruising in potatoes is a kind of damage caused by the mechanical impact, which is the major cause of post-harvest losses. This kind of injury is hard to detect because it occurs due to the internal cellular rupture of the tuber. This research aims at evaluating the applicability of the biospeckle laser technique in order to detect and predict the internal bruising in potatoes caused by mechanical impacts during the early stages where there is no visual perception of the injury. The correlation of Pearson between a quantitative index generated from the biospeckle laser images (BSL values) and the enzymatic darkening in the region of the injury have been calculated. The BSL values was used to compare a group of potatoes subjected to mechanical impacts caused by a pendulum and a group of potatoes that did not suffer any sort of mechanical impacts. Comparison was performed by average and the standard error of the BSL values was obtained: Before impact, immediately after, 2, 4, 6, 24, 48 and 72 h after the mechanical impact. Results showed that the BSL values correlated significantly with the progressive darkening of the potato tissue (r =-0.79) and this allowed the detection of the formation of internal bruising from 4 hours after the occurrence of a mechanical impact.

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Monitoring of the action of drugs in melanoma cells by dynamic laser speckle

Cited by 25 publications

References 27 publications

Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image

Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image

Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

Early prediction of internal bruising in potatoes by biospeckle laser technique

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