Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

Ansari, Mohammad Zaheer; Ramírez-Miquet, Evelio E.; Otero, Isabel Diz; Rodríguez, Dania; Darias, J. G.

doi:10.1117/1.jbo.21.6.066006

Cited by 27 publications

(19 citation statements)

References 18 publications

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“…8c ). This framework, while aimed at FLN, could easily be applied to other soil pests such as microbes or fungi, which have already been shown to be detectable by BSL 30 , 32 , 126 . Furthermore, the biocompatibility of Ludox® and easy manipulation of chemical properties in terms of nutrients and pH of transparent soil make it a suitable substrate for a wide range of soil-dwelling microorganisms.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, light sheet based techniques such as SPIM (Selective Plane Illumination Microscopy) have shown great potential for live imaging studies 24 – 28 , but light scattering itself may not allow easy separation of nematode and root in the image 29 . On the other hand, techniques based on the dynamic light scattering created by living organisms, termed biospeckle, have shown potential as a simple, rapid and non-invasive means of tracking biological activity 30 – 32 . Biospeckle has the advantage of enhancing the contrast between living organisms from their abiotic background.…”

Section: Introductionmentioning

confidence: 99%

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New live screening of plant-nematode interactions in the rhizosphere

O’Callaghan

Braga

Neilson

et al. 2018

Sci Rep

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Free living nematodes (FLN) are microscopic worms found in all soils. While many FLN species are beneficial to crops, some species cause significant damage by feeding on roots and vectoring viruses. With the planned legislative removal of traditionally used chemical treatments, identification of new ways to manage FLN populations has become a high priority. For this, more powerful screening systems are required to rapidly assess threats to crops and identify treatments efficiently. Here, we have developed new live assays for testing nematode responses to treatment by combining transparent soil microcosms, a new light sheet imaging technique termed Biospeckle Selective Plane Illumination Microscopy (BSPIM) for fast nematode detection, and Confocal Laser Scanning Microscopy for high resolution imaging. We show that BSPIM increased signal to noise ratios by up to 60 fold and allowed the automatic detection of FLN in transparent soil samples of 1.5 mL. Growing plant root systems were rapidly scanned for nematode abundance and activity, and FLN feeding behaviour and responses to chemical compounds observed in soil-like conditions. This approach could be used for direct monitoring of FLN activity either to develop new compounds that target economically damaging herbivorous nematodes or ensuring that beneficial species are not negatively impacted.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New live screening of plant-nematode interactions in the rhizosphere

O’Callaghan

Braga

Neilson

et al. 2018

Sci Rep

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“…Biological samples show this behavior when they are illuminated with a laser. However, there are other dynamic processes where this behavior is also observed [46]. As stated, during phase separation, the domain surfaces are changing due to coalescence, thus giving dynamic surfaces.…”

Section: Introductionmentioning

confidence: 73%

“…It is used to characterize movements within a row of images in time. The MHI indicates a static pattern that the pixel intensity is a function of the recent image sequence motion and contains the fundamental information in order to determine the motion of the object during the activity 45,46 . MHI has been used profoundly for visualizing the perfusion changes locations during different stimuli 47 .…”

Section: Numerical Processingmentioning

confidence: 99%

Revealing the structure deformation in multi-component lipids by dynamic speckle pattern analysis

Panahi

Rad

Sasan

et al. 2021

Preprint

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In the bio-molecular field of Liquid-crystalline, the stacked lipid bilayers phase represents an extensive issue. It was proven that in addition to the typical smectic order, multi-component multilayer membranes could present columnar order arising from the coupling of two-dimensional intralayer phase separation. Interlayer smectic ordering distributes across hundreds of membrane lamellae, resulting in long-range alignment of phase-separated domains. In the current paper, the self-ordering of the sample over time is investigated by dynamic speckle pattern analysis. Temporal alteration of the sample is associated with changes in the intensity and contrast of the speckle pattern. We have used different factors, including motion history, co-occurrence, and time history speckle pattern, to examine the structure evolution of lipid mixtures, which are done by sequence images of speckle pattern over time. Roughness parameters inclusive of skewness and kurtosis of speckle pattern were investigated that are related to the domain size evolution of the sample. It is shown that the activity is decreasing by increasing the cholesterol content and inertia moment is inversely proportional with the growth of domain formation. The experimental setup, data recording, and data analysis are presented too.

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“…This method can be efficient for bacterial activity rather than the graphical methods used to monitor activities regarding the inhomogeneity of the sample. 27…”

Section: Analysis In Subregion Of Interestsmentioning

confidence: 99%

Dynamic laser speckle analyzed considering inhomogeneities in the biological sample

2017

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Dynamic laser speckle phenomenon allows a contactless and nondestructive way to monitor biological changes that are quantified by second-order statistics applied in the images in time using a secondary matrix known as time history of the speckle pattern (THSP). To avoid being time consuming, the traditional way to build the THSP restricts the data to a line or column. Our hypothesis is that the spatial restriction of the information could compromise the results, particularly when undesirable and unexpected optical inhomogeneities occur, such as in cell culture media. It tested a spatial random approach to collect the points to form a THSP. Cells in a culture medium and in drying paint, representing homogeneous samples in different levels, were tested, and a comparison with the traditional method was carried out. An alternative random selection based on a Gaussian distribution around a desired position was also presented. The results showed that the traditional protocol presented higher variation than the outcomes using the random method. The higher the inhomogeneity of the activity map, the higher the efficiency of the proposed method using random points. The Gaussian distribution proved to be useful when there was a well-defined area to monitor.

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Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

Cited by 27 publications

References 18 publications

New live screening of plant-nematode interactions in the rhizosphere

New live screening of plant-nematode interactions in the rhizosphere

Revealing the structure deformation in multi-component lipids by dynamic speckle pattern analysis

Dynamic laser speckle analyzed considering inhomogeneities in the biological sample

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