Monensin action on the Golgi complex in perfused rat liver: Evidence against bile salt vesicular transport

Reynier, M.O.; Hashieh, Imad Abou; Crotté, C.; Carbuccia, Nadine; Richard, B.C; Gérolami, A

doi:10.1016/0016-5085(92)90328-v

Cited by 19 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The hepatic secretion of biliary phospholipids is poorly understood, but phosphatidylcholine and cholesterol appear to be co‐transported from the smooth endoplasmic reticulum to the canalicular plasma membrane, partly by microtubule‐mediated vesicular movement, but predominantly by cytosolic lipid transfer proteins 59 ,. 60 Under experimental conditions whereby the hepatic synthesis of cholesterol is increased during a controlled rate of bile acid secretion, 61 there is a linear increase in the biliary percentage of phosphatidylcholine 16:0–18:1 and 16:0–20:4.…”

Section: Discussionmentioning

confidence: 99%

Bile composition in inflammatory bowel disease: ileal disease and colectomy, but not colitis, induce lithogenic bile

Pereira

Bain

Kumar

et al. 2003

Aliment Pharmacol Ther

View full text Add to dashboard Cite

SUMMARYBackground: Inflammatory bowel disease is a risk factor for gall-bladder stones, but there is controversy about the composition of these stones and whether such patients develop lithogenic bile. Methods: In 54 gallstone-free inflammatory bowel disease patients and 13 non-inflammatory bowel disease patients with cholesterol-rich gallstones, we measured the biliary cholesterol saturation indices, nucleation times and bilirubin concentrations, and determined the bile acid composition and molecular species of phosphatidylcholine, in gall-bladder bile. Results: Patients with Crohn's colitis or ulcerative colitis had less saturated bile (mean cholesterol saturation index, 0.9) and longer nucleation times (median,

show abstract

Section: Discussionmentioning

confidence: 99%

Bile composition in inflammatory bowel disease: ileal disease and colectomy, but not colitis, induce lithogenic bile

Pereira

Bain

Kumar

et al. 2003

Aliment Pharmacol Ther

View full text Add to dashboard Cite

show abstract

“…However, experiments with microtubule inhibitors have shown that biliary lipid secretion depends on vesicular transport [151][152][153], but neither the nature nor the intracellular transport route of these vesicles have been elucidated with certainty [150]. Also, an inhibitory effect of monensin on biliary lipid secretion has been reported [154], but this was not confirmed by others [155]. Some evidence points towards the Golgi apparatus as an assembly point for lipids and bile acids that are destined for biliary secretion.…”

Section: Lipid Transport To the Canalicular Membranementioning

confidence: 99%

“…In couplets or perfused rat liver, biliary lipid secretion is not influenced by treatments that block the transcytotic delivery of horseradish (Armoracia rusticana) peroxidase (BFA, monensin), indicating that the transcytotic pathway has no major physiological relevance in the process of lipid secretion into the bile [155,158]. Thus these results would argue against co-transport of lipids (destined for biliary secretion) and apical proteins because, as noted above, in hepatocyes transport of proteins to the apical membrane domain largely depends on transcytosis for apical delivery.…”

Section: Lipid Transport To the Canalicular Membranementioning

confidence: 99%

Mechanisms and functional features of polarized membrane traffic in epithelial and hepatic cells

Zegers

Hoekstra

1998

Biochemical Journal

135

View full text Add to dashboard Cite

Epithelial cells express plasma-membrane polarity in order to meet functional requirements that are imposed by their interaction with different extracellular environments. Thus apical and basolateral membrane domains are distinguished that are separated by tight junctions in order to maintain the specific lipid and protein composition of each domain. In hepatic cells, the plasma membrane is also polarized, containing a sinusoidal (basolateral) and a bile canalicular (apical)-membrane domain. Relevant to the biogenesis of these domains are issues concerning sorting, (co-)transport and regulation of transport of domain-specific membrane components. In epithelial cells, specific proteins and lipids, destined for the apical membrane, are sorted in the trans-Golgi network (TGN), which involves their sequestration into cholesterol/sphingolipid 'rafts', followed by 'direct' transport to the apical membrane. In hepatic cells, a direct apical transport pathway also exists, as revealed by transport of sphingolipids from TGN to the apical membrane. This is remarkable, since in these cells numerous apical membrane proteins are 'indirectly' sorted, i.e. they are first transferred to the basolateral membrane prior to their subsequent transcytosis to the apical membrane. This raises intriguing questions as to the existence of specific lipid rafts in hepatocytes. As demonstrated in studies with HepG2 cells, it has become evident that, in hepatic cells, apical transport pathways can be regulated by protein kinase activity, which in turn modulates cell polarity. Finally, an important physiological function of hepatic cells is their involvement in intracellular transport and secretion of bile-specific lipids. Mechanisms of these transport processes, including the role of multidrug-resistant proteins in lipid translocation, will be discussed in the context of intracellular vesicular transport. Taken together, hepatic cell systems provide an important asset to studies aimed at elucidating mechanisms of sorting and trafficking of lipids (and proteins) in polarized cells in general.

show abstract

“…Large domains of coiled-coils have also been noted in other autoantigens including the nuclear mitotic antigen (NuMA) (27), lamin B (28), and myosin heavy chain (29), and one or two coiled-coils have been noted in the 52-kDa SS-A/Ro (30) and 80/86-kDa Ku antigens (31). This motif has also been described in a number of other prokaryotic and eukaryotic proteins, most notably Golgi complex proteins (32,33), the streptococcal M protein (29), products of the nuclear oncogenes c-fos and c-jun (34), viral fusogenic proteins (35), and the envelope glycoproteins of HIV1 and other viruses (36). Although certain proteins with coiled-coils are able to bind DNA and are thought to be predominantly regulatory in their function (37), this structure also mediates the dimerization of certain transactivators (38) and is essential for recombination of certain viral proteins (39) and oligomerization of viral envelope proteins (40).…”

Section: Isolation and Characterization Of Cdnas Encodingmentioning

confidence: 99%

Molecular Characterization of Golgin-245, a Novel Golgi Complex Protein Containing a Granin Signature

Fritzler

Lung

Hamel

et al. 1995

Journal of Biological Chemistry

100

View full text Add to dashboard Cite

The serum from a Sjö gren's syndrome patient with anti-Golgi antibodies was used as a probe to isolate a 4.6-kilobase pair cDNA insert from a HeLa cDNA library. Expression of the cDNA in Escherichia coli and the in vitro translation products of the cDNA yielded a recombinant protein that migrated in SDS-polyacrylamide gel electrophoresis at 180 kDa. This protein was immunoprecipitated by the human anti-Golgi serum and by immune rabbit serum but not by normal human serum or preimmune rabbit serum. Western blot analysis showed that the prototype human and immune rabbit sera recognized a 245-kDa protein, suggesting that the isolated clone contained a partial cDNA. The 5-upstream sequence obtained by the rapid amplification of cDNA ends methodology using human placental cDNA and the combined HeLa cDNA contained 6965 base pairs and encoded a protein of 245 kDa and, like other Golgi autoantigens described earlier, is highly rich in coiledcoils. The deduced amino acid sequence included the decapeptide ESLALEELEL, which was identified as one of two signature sequences previously reported in a family of peptide hormones and neuropeptides known as "granins." This is the first report of a Golgi complex autoantigen that bears structural similarities to the granin family of proteins.

show abstract

Monensin action on the Golgi complex in perfused rat liver: Evidence against bile salt vesicular transport

Cited by 19 publications

References 31 publications

Bile composition in inflammatory bowel disease: ileal disease and colectomy, but not colitis, induce lithogenic bile

Bile composition in inflammatory bowel disease: ileal disease and colectomy, but not colitis, induce lithogenic bile

Mechanisms and functional features of polarized membrane traffic in epithelial and hepatic cells

Molecular Characterization of Golgin-245, a Novel Golgi Complex Protein Containing a Granin Signature

Contact Info

Product

Resources

About