Molecular Weight and Molecular Weight Distribution of Unfractionated Yeast Transfer Ribonucleic Acid¹

Abstract

Claesson and Dr. F. Friedberg for loans of the osmometers.

Design of an Oligosaccharide Scaffold That Binds in the Minor Groove of DNA

“…An absorbance was measured and the concentration of RNA was determined using a millimolar extinction coefficient of 570 mM -1 cm -1 at 260 nm. 22 DNA binding was conducted at 15 mM NaCl, 25 mM Tris, pH 7.5. RNA binding was conducted at 50 mM KCl, 50 mM potassium cacodylate, pH 7.0.…”

Conformational studies on transfer ribonucleic acid. Fluorescence lifetime and nanosecond depolarization measurements on bound ethidium bromide

“…This may correspond to any one of ratios , , or j%. If the macromole- »The relaxation times were corrected to 25°and the viscosity of water at that temperature.6 Calculated from partial specific volume v = 0.503 ml/g (D. D. Henley, T. Lindahl, and J. R. Fresco, unpublished observations quoted by Adams et al, 1967), and molecular weight = 26,400 g/mole (Lindahl et al, 1965). 6 Using a degree of hydration ß = 1.2 g of H20/g of tRNA (I. D. Kuntz and J. R. Fresco, private communication).…”

“…tRNA:EB A Unfractionated yeast tRNA 2.6 1.0 x io-4 0.04 5 X IO"6 2.0 B Unfractionated yeast tRNA 1.5 5.7 X 10-5 0.003 5 X 1.1 C Unfractionated yeast tRNA 2.6 1.0 X 10~4 GO» 5 X IO"5 2.0 D Yeast tRNAphe 1.8 6.8 X 0.0026 6.4 X IO"6 1.1 E Yeast tRNAphe 1.8 6.8 X 0.06 5.8 X IO-5 1.2 0 Molecular weight of bulk yeast tRNA was taken to be 26,400 g/mole (Lindahl et al, 1965). Molecular weight of 24,000 g/mole was estimated from the published nucleic acid sequence of tRNAphe (RajBhandary et al, 1967).b Contains 0.001 m NaCl instead.…”

Isolation and Characterization of Low Molecular Weight Ribonucleic Acid Species from Bacillus subtilis*

“…Concentrations of tRNA and 5S RNA were determined by ultraviolet absorption (Al¡s 215). This extinction value is that used for pure tRNA (Lindahl et al, 1965), and it was assumed that 5S RNA would not differ greatly from this value. The concentration of NaCl in MAK column effluents was determined by measurement of refractive indices.…”