Isolation and Characterization of Low Molecular Weight Ribonucleic Acid Species from Bacillus subtilis<sup>*</sup>

Morell, Pierre; Smith, Issar; Dubnau, David; Marmur, Julius

doi:10.1021/bi00853a040

Cited by 64 publications

(15 citation statements)

References 20 publications

(16 reference statements)

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“…1 that the two preparations contained approximately equal proportions of hostspecific sRNA, thus showing no contamination from phage-specific RNA. Furthermore, since the hybridization curves showed saturation of binding sites at about 0.04% of the DNA, a value which is in close agreement with published values for E. coli sRNA (4,6,12), there could be no significant contamination by degraded host cell high-molecular-weight ribonucleates, since such contamination would lead to greatly increased values for saturation of DNA-binding sites.…”

Section: Resultssupporting

confidence: 88%

Synthesis of Soluble Ribonucleates in Escherichia coli Infected with Bacteriophage R17

Hudson

Paranchych

1968

J Virol

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Section: Resultssupporting

confidence: 88%

Synthesis of Soluble Ribonucleates in Escherichia coli Infected with Bacteriophage R17

Hudson

Paranchych

1968

J Virol

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“…pBD15 and pBD16 are derivatives of pE194 carrying the cop-6 and tyc-l mutations, respectively (10). B. subtilis strains were grown in VY broth (18) to late logarithmic phase for the isolation of ribosomes, and to mid-log for the induction experiments. For enzyme purification, bacteria were grown in YPT medium (19) (9).…”

Section: Strains Media and Growth Conditionsmentioning

confidence: 99%

Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance

Shivakumar¹,

Dubnau²

1981

Nucl Acids Res

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The ermC gene of plasmid pE194 specifies resistance to the macrolidelincosamide-streptogramin B antibiotics. This resistance, as well as synthesis of the 29,000 dalton protein product of ermC,has been shown to be induced by erythromycin. Weisblum-and his colleagues have established that macrolide resistance is associated with a specific dimethylation of adenine in 23 S rRNA. We show that pE194 specifies an RNA methylase that can utilize either 50 S ribosomes or 23 S rRNA as substrates. Synthesis of this methylase is induced by low concentrations of erythromycin, and the enzyme is produced in elevated amounts by strains carrying a high copy number mutant of pEl94. The methylase comigrates with the 29K ermC product on polyacrylamide gels. The purification and some properties of this methylase are described.

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“…RNA isolation. RNA was isolated from cells harvested during exponential growth (2 x 108 to 5 x 108 cells per ml) in minimal medium (5) or VY (13) and from cells harvested at various times after transfer-to minimal sporulation medium (19). Methods for the preparation of RNA and treatment with RNase-free DNase have been previously described (9).…”

mentioning

confidence: 99%

Bacillus subtilis spo0H gene

Weir¹,

Dubnau²,

Ramakrishna³

et al. 1984

J Bacteriol

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A 2.8-kilobase fragment of the Bacillus suibtilis chromosome containing a functional spoOH gene was cloned by using a modification of the helper system described by T. Gryczan and co-workers (T. Gryczan, S. Contente, and D. Dubnau, Mol. Gen. Genet. 177:459-467, 1980). The chromosomal segment specifically complements spoOH mutations in recE4 strains and when integrated into the chromosome of Rec+ strains maps in the spoOH region of the B. subtilis genome. A deletion within the transcribed region of the cloned spoOH gene was constructed which abolishes its spoOH+-complementing activity. DNA sequences containing this deletion were introduced into a B. suibtilis Rec+ strain containing the spoOH75 mutation. The absence of recombination between the deletion and the spo0H mutation indicates that both reside in the same gene. There is homology between the B. slubtilis spoOH gene and a 1.2-kilobase chromosomal fragment from Bacillus licheniformis which also complements B. suibtilis spoOH mutations. In vivo transcription mapping experiments have shown that the B. suibtilis spoOH gene is transcribed during vegetative growth as

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Isolation and Characterization of Low Molecular Weight Ribonucleic Acid Species from Bacillus subtilis^*

Cited by 64 publications

References 20 publications

Synthesis of Soluble Ribonucleates in Escherichia coli Infected with Bacteriophage R17

Synthesis of Soluble Ribonucleates in Escherichia coli Infected with Bacteriophage R17

Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance

Bacillus subtilis spo0H gene

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Isolation and Characterization of Low Molecular Weight Ribonucleic Acid Species from Bacillus subtilis*

Cited by 64 publications

References 20 publications

Synthesis of Soluble Ribonucleates in Escherichia coli Infected with Bacteriophage R17

Synthesis of Soluble Ribonucleates in Escherichia coli Infected with Bacteriophage R17

Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance

Bacillus subtilis spo0H gene

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Isolation and Characterization of Low Molecular Weight Ribonucleic Acid Species from Bacillus subtilis^*