Molecular typing, virulence traits and risk factors of pandrug-resistant Acinetobacter baumannii spread in intensive care unit centers of Baghdad city, Iraq

Kanaan, Manal Hadi Ghaffoori; Khashan, Hawazin Thamir

doi:10.1097/mrm.0000000000000282

Cited by 11 publications

(9 citation statements)

References 34 publications

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“… 30.2% Liu et al., 2016 FimH Type I Fimbriae protein Bacterial cell adhesion 6.8 – 50% Abdullah and Ahmed, 2019 ; Bentancor et al., 2012 ; Padmaja et al., 2020 epsA Polysaccharide export outer membrane protein Potentially plays a role in the export of polysaccharides during biofilm formation. 95% Russo et al., 2010 ; Zeighami et al., 2019 Ptk/wzc Putative tyrosine kinase Plays a role in K1 capsular polysaccharide production 95% Russo et al., 2010 ; Zeighami et al., 2019 csgA Curli specific gene A Amyloidogenic proteins contribute to matrix formation 20.54%−70% Jaisankar et al., 2020 kpsMII Group 2 capsule synthesis Production of capsule 57–75% Kanaan et al., 2021 ; Zeighami et al., 2019 , Pap Pili system Homologous to E. coli pili and associated with A. baumannii biofilm formation 80% Kanaan et al., 2021 ; Li et al. 2017 Prp Photoregulated pilus system biofilm formation in response to light NA Wood et al., 2018 Type IV pili Pili system adhesion to host cells and stainless steel NA Ronish et al., 2019 LHp2–11,085 Gene encoding for protein Attachment to biotic and abiotic surfaces NA Zarrilli et al., 2016 RecA DNA repair protein Negatively regulates biofilm formation through bfmR and causes dispersal of cells.…”

Section: Strategies For Controlling Biofilm-associated a Ba...mentioning

confidence: 99%

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Upmanyu

Haq

Singh

2022

Current Research in Microbial Sciences

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Section: Strategies For Controlling Biofilm-associated a Ba...mentioning

confidence: 99%

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Upmanyu

Haq

Singh

2022

Current Research in Microbial Sciences

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“…This study included 60% MDR A. baumannii VAP isolates. Kanaan and Khashan revealed high MDR percentage among A. baumannii isolated from ICU (75%) 26 . Bahador et al 27 reported that the MDR A. baumannii isolates obtained from 3 different hospitals were 91%, 89%, and 75%.…”

Section: Discussionmentioning

confidence: 98%

Virulence Profile of Acinetobacter baumannii Isolated from Ventilator-Associated Pneumonia Cases

Attia,

Ghazal,

Gaballah

et al. 2024

Egyptian Journal of Medical Microbiology

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Background: Acinetobacter baumannii (A. baumannii) is an opportunistic bacterium with high antimicrobial resistance and a complex set of virulence factors. It can cause hospital-acquired infections such as ventilator-associated-pneumonia (VAP).The study aimed to detect some virulence factors for A. baumannii isolated from VAP samples phenotypically and genotypically and relating them to antimicrobial resistance. Methodology: Thirty A. baumannii VAP isolates were obtained and tested for antimicrobial sensitivity using the disc-diffusion method. Polystyrene adherence replica method was used to detect bacterial adherence. Capsules were screened using the Anthony capsular staining method. Biofilm forming capacity and serum resistance were also determined. Fifteen genes encoding for either adhesive or non-adhesive virulence factors were detected using multiplex PCR. Results: All A. baumannii isolates showed a high antimicrobial resistance pattern with 60% being multi-drug resistant. Most isolates showed polystyrene adherence; 33.3%, 20% and 26.7% were of high, moderate and low adherence, respectively. All isolates were biofilm formers; 73.3% were moderate or strong biofilm formers. All isolates except 4 were encapsulated and 86.7% showed serum resistance. Most isolates had a high number of virulence genes which correlated significantly with the detected phenotypic virulence factors and multi-drug resistance. Conclusion: A. baumannii VAP isolates could-in a variable degree -adhere to polystyrene, produce biofilm, produce capsule and have serum resistance. Most of these virulence factors were associated with multidrug-resistance and mostly correlated with adhesive and/or non-adhesive virulence genes. A virulence profile was introduced for these isolates that highlights the hazard of this highly resistant and virulent bacterium.

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“…The previous studies in Iraq confirmed this relationship by investigating the MDR phenomenon in many foodborne pathogens [ 21 , 48 – 51 ]. Other studies in Iraq also pointed to wrong practices regarding the abuse and misuse of antibiotics, which drove the exacerbation of this phenomenon [ 52 – 54 ]. Accordingly, these results highlighted the importance of preventing antibiotic overuse in reducing the MDR of Salmonella spp.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Salmonella enterica serovars Enteritidis and Typhimurium isolated from retail chicken meat in Wasit markets, Iraq

Kanaan

2023

Vet World

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Background and Aim: Food poisoning caused by Salmonella enterica serovars is the most common type of foodborne illness. Tainted chicken meat is a major vector for spreading these serovars throughout the food supply chain. Salmonella isolates that developed resistance to commonly used antimicrobials pose a noteworthy risk to public health, yet there has been a lack of data on this issue in Iraq. Therefore, it is crucial to address these serious public health challenges with an adequate database on the occurrence and antibiotic resistance of these serovars. This study aimed to determine the frequency of occurrence of Salmonella enterica serovars Enteritidis and Typhimurium (S. Enteritidis and S. Typhimurium), antimicrobial resistance (AMR), and prevalence of multidrug resistance among S. Enteritidis and S. Typhimurium isolated from poultry meat collected in Wasit Province in Iraq. Materials and Methods: A total of 150 raw and frozen poultry meat samples were gathered from retail markets in various locales across the Wasit Governorate in Iraq. Salmonella spp. were successfully cultured and identified using the technique recommended by ISO 6579:2002, with minor modifications. The multiplex polymerase chain reaction approach was used to confirm Salmonella spp. (S. Enteritidis and S. Typhimurium). A disk diffusion test was performed to determine the susceptibility to particular antimicrobial agents, and 12 different antimicrobial agents were evaluated. Results: Only 19 of the 150 (12.7%) samples tested positive for Salmonella (16% and 11% were isolated from raw and frozen chicken meat, respectively). S. Enteritidis accounted for 63.2%, whereas S. Typhimurium accounted for 36.8%. Nalidixic acid resistance was the most common (73.7%), followed by sulfamethoxazole-trimethoprim (63.2%) and tetracycline (63.2%), but gentamicin and ciprofloxacin (up to 15.8%) only had modest resistance. Antibiogram of S. Enteritidis and S. Typhimurium yield 13 antibiotypes. Among the 19 Salmonella isolates, 12 of 19 (63.2%) established resistance to no less than three categories of antimicrobials. Conclusion: This study highlighted the necessity of limiting the utilization of antibiotics in animal production by providing vital information regarding the frequency and AMR of Salmonella at markets in Wasit Province. Therefore, risk assessment models could use these data to lessen the amount of Salmonella passed on to humans in Iraq from chicken meat. Keywords: antimicrobial resistance, chicken, food poisoning, serovars.

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Molecular typing, virulence traits and risk factors of pandrug-resistant Acinetobacter baumannii spread in intensive care unit centers of Baghdad city, Iraq

Cited by 11 publications

References 34 publications

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Virulence Profile of Acinetobacter baumannii Isolated from Ventilator-Associated Pneumonia Cases

Prevalence and antimicrobial resistance of Salmonella enterica serovars Enteritidis and Typhimurium isolated from retail chicken meat in Wasit markets, Iraq

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