Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Upmanyu, Kirti; Haq, Prof. Qazi Mohd. Rizwanul; Singh, Ruchi

doi:10.1016/j.crmicr.2022.100131

Cited by 27 publications

(25 citation statements)

References 188 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the bap gene was detected in 36.4%, 53%, and 72% of weak, moderate, and strong biofilm producers, respectively. This has previously been confirmed in studies that demonstrated the role of the Bap protein in biofilm maturation rather than in the initial stage of adherence [ 46 ]. Authors from Iran, Thailand, and Korea found ompA in 81%, 84.4%, and 68.8% of isolates, respectively [ 38 , 45 , 47 ], which corresponds to our finding (68.8%).…”

Section: Discussionsupporting

confidence: 58%

Carbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles

et al. 2023

View full text Add to dashboard Cite

This study aimed to investigate the biofilm-production ability of carbapenem-resistant Acinetobacter baumannii (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedded CRAB, and the relationship between biofilm production and bacterial genotypes. A total of 111 CRAB isolates were tested for antimicrobial susceptibility, biofilm formation, presence of the genes encoding carbapenemases, and biofilm-associated virulence factors. The antibiofilm effects of disinfectants and SeNPs against CRAB isolates were also tested. The vast majority of the tested isolates were biofilm producers (91.9%). The bap, ompA, and csuE genes were found in 57%, 70%, and 76% of the CRAB isolates, with the csuE being significantly more common among biofilm producers (78.6%) compared to non-biofilm-producing CRAB (25%). The tested disinfectants showed a better antibiofilm effect on moderate and strong biofilm producers than on weak producers (p < 0.01). The SeNPs showed an inhibitory effect against all tested planktonic (MIC range: 0.00015 to >1.25 mg/mL) and biofilm-embedded CRAB, with a minimum biofilm inhibitory concentration of less than 0.15 mg/mL for 90% of biofilm producers. In conclusion, SeNPs might be used as promising therapeutic and medical device coating agents, thus serving as an alternative approach for the prevention of biofilm-related infections.

show abstract

Section: Discussionsupporting

confidence: 58%

Carbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Efflux pumps play a critical role in the pathophysiology of many human pathogens. − What makes bacterial efflux pumps so special is broad substrate specificity, that is, a single type of efflux pump can expel multiple antibiotics. Active expression of a single efflux pump can cause treatment failure against multiple antibiotics. , Furthermore, efflux pumps also play an important role in biofilm formation by transporting various biofilm matrix components …”

Section: Discussionmentioning

confidence: 99%

Small Molecule IITR08367 Potentiates Antibacterial Efficacy of Fosfomycin against Acinetobacter baumannii by Efflux Pump Inhibition

Saini,

Gaurav,

Hussain

et al. 2024

ACS Infect. Dis.

View full text Add to dashboard Cite

Fosfomycin is a broad-spectrum single-dose therapy approved for treating lower urinary tract infections. Acinetobacter baumannii, one of the five major UTI-causing pathogens, is intrinsically resistant to fosfomycin. Reduced uptake and active efflux are major reasons for this intrinsic resistance. AbaF, a major facilitator superfamily class of transporter in A. baumannii, is responsible for fosfomycin efflux and biofilm formation. This study describes the identification and validation of a novel smallmolecule efflux pump inhibitor that potentiates fosfomycin efficacy against A. baumannii. An AbaF inhibitor screening was performed against Escherichia coli KAM32/pUC18_abaF, using the noninhibitory concentration of 24 putative efflux pump inhibitors. The inhibitory activity of IITR08367 [bis(4-methylbenzyl) disufide] against fosfomycin/H + antiport was validated using ethidium bromide efflux, quinacrine-based proton-sensitive fluorescence, and membrane depolarization assays. IITR08367 inhibits fosfomycin/H + antiport activity by perturbing the transmembrane proton gradient. IITR08367 is a nontoxic molecule that potentiates fosfomycin activity against clinical strains of A. baumannii and prevents biofilm formation by inhibiting efflux pump (AbaF). The IITR08367-fosfomycin combination reduced bacterial burden by > 3 log 10 in kidney and bladder tissue in the murine UTI model. Overall, fosfomycin, in combination with IITR08367, holds the potential to treat urinary tract infections caused by A. baumannii.

show abstract

“…Clinical isolates were sensitive only to colistin. Because of the seriousness of the problem with A. baumannii , especially in the light of its strong tendency to form biofilms ( Upmanyu et al, 2022 ), treatment alternatives are urgent to be found and better understood. The pre-screening of antibacterial activity of cinnamon EO has shown a notable antimicrobial effect against A. baumannii which is in accordance with data from literature ( Chouhan et al, 2017 ; Intorasoot et al, 2017 ; Aumeeruddy-Elalfi et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii

et al. 2022

View full text Add to dashboard Cite

Acinetobacter baumannii is an emerging nosocomial pathogen resistant to a wide spectrum of antibiotics, with great potential to form a biofilm, which further aggravates treatment of infections caused by it. Therefore, searching for new potent agents that are efficient against A. baumannii seems to be a necessity. One of them, which has already been proven to possess a wide spectrum of biological activities, including antimicrobial effect, is cinnamon essential oil. Still, further increase of antibacterial efficacy and improvement of bioavailability of cinnamon oil is possible by emulsification process. The aim of this study was comparative analysis of cinnamon essential oil and its emulsion against biofilm forming A. baumannii clinical isolates. Furthermore, the investigation of toxicological aspects of possible applications of essential oil and emulsion was done as well. Gas chromatography–mass spectrometry of essential oil indicated trans-cinnamaldehyde as the most abundant component. The cinnamon emulsion was synthesized from cinnamon essential oil by combining modified low- and high- energy methods. Synthesized emulsion was characterized with Fourier-transform infrared spectroscopy and photon correlation spectroscopy. Both substances exhibited significant antibacterial (minimal inhibitory concentrations in the range 0.125–0.5 mg/ml) and antibiofilm effects (inhibitions of formation and reduction of pre-formed biofilm were 47–81 and 30–62%, respectively). Compared to essential oil, the efficacy of emulsion was even stronger considering the small share of pure oil (20%) in the emulsion. The result of biofilm eradication assay was confirmed by scanning electron microscopy. Even though the cytotoxicity was high especially for the emulsion, genotoxicity was not determined. In conclusion, strong antibacterial/antibiofilm effect against A. baumannii of the cinnamon essential oil and the fact that emulsification even potentiated the activity, seems to be of great significance. Observed cytotoxicity implicated that further analysis is needed in order to clearly determine active principles being responsible for obtained antibacterial/antibiofilm and cytotoxic properties.

show abstract

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Cited by 27 publications

References 188 publications

Carbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles

Carbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles

Small Molecule IITR08367 Potentiates Antibacterial Efficacy of Fosfomycin against Acinetobacter baumannii by Efflux Pump Inhibition

Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii

Contact Info

Product

Resources

About