Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan

Abstract

Introduction: Over the past decade methicillin-resistant Staphylococcus aureus (MRSA) has been recognized as a major cause of healthcare associated infections. Recently, however, epidemiology of this pathogen has changed drastically with the emergence of new clones in the community. Efficient epidemiological typing methods are essential to monitor and limit the occurrence and spread of epidemic clones. Methodology: A total of sixty S. aureus isolates were collected from the Jordan University hospital in Amman-Jordan. All isolates were characterized using Staphylococcus protein A (spa) typing and pulsed-field gel electrophoresis (PFGE). Samples were tested for their susceptibility patterns against seven antimicrobial agents and for their potential to form biofilms. Results: spa typing showed that spa type t044 was the most common representing 28% of the isolates studied and 38% of the MRSA population. PFGE revealed fourty-six pulsotypes among the sixty tested isolates clustering similar spa types together. The predominant resistance was detected against levofloxacin, chloramphenicol and clindamycin. One MSSA isolate typed as spa t955 showed biofilm formation potential through protein deposition.. Conclusion: The study results are based on one hospital, but the findings of this and other studies conducted in the region indicate that there is an urgent need for standardized surveillances combined with the application of well-validated typing methods to assess the occurrence of MRSA and to control its spread.

Relevance of vancomycin suceptibility on patients outcome infected with Staphylococcus aureus

“…The rest of the antimicrobials for both types of staphylococci showed no significant differences (Figure 3) Susceptibility rates to the other antimicrobials were evaluated among outpatient MRSA isolates, these were 74% for quinolones and 69% TMP/ SMX, respectively. An earlier study carried out in the Jordan University Hospital demonstrated that MRSA susceptibility to quinolones was 15%, clindamycin 68%, and gentamicin 68%,respectively [35]. In the current study, a relatively higher susceptibility rates were observed for quinolones 74%, clindamycin 56%, and gentamycin 94%, respectively.…”

Antibiograms, toxin profiling and molecular typing of Staphylococcus aureus isolates from two tertiary hospitals in Jordan

“…MRSA is one of the most commonly isolated pathogen associated with general and nosocomial infections worldwide including Jordan [5,24]. The incidence of MRSA varies by location from one institution to another, and from Country to another.…”

“…Staphylococcus aureus is one of the most commonly isolated human bacterial pathogen and is responsible for causing a battery of diseases ranging from mild superficial infections such as skin infections to severe life-threatening infections such as, endocarditis, deep-seated abscesses, pneumonia and septicemia [1][2][3][4][5]. The ability of S. aureus to cause a spectrum of diseases both in humans and animals is due to a combination of bacterial virulence factors [6].…”

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

“…In Kuwait, the reported proportions of MRSA-ST80 among MRSA strains were 38 [45]. In Jordan, MRSA-ST80 strains were detected in 34.1% (14/41) of all MRSA strains recovered from infections from 2009 to 2010 [46]. Two studies reported that MRSA-ST80 strains represented 9.8% (4/41) and 5.4% (2/37) of all MRSA strains recovered from nasal and fecal carriage, respectively [47,48].…”