Molecular Typing of Campylobacter jejuni and Campylobacter coli of Human Strains Isolated in Turkey Over an Eight-Year Period

İlktaç, Mehmet; Öngen, Betigül

doi:10.7754/clin.lab.2019.190613

Cited by 5 publications

(4 citation statements)

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“…However, the rate of detection is lower than that reported in other studies carried out in South Africa, Belgium, Denmark, Sweden, and Canada [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. The distribution of species found in this study that revealed the predominance of C. jejuni and C. coli is similar to the previous findings of our laboratory using classical culture techniques and incubation conditions that were optimal for the growth of thermotolerant species [ 31 ]. Moreover, the overall isolation rate of Campylobacter spp.…”

Section: Discussionsupporting

confidence: 89%

“…The hippurate hydrolysis test, one of the most frequently used tests that discriminates C. jejuni from other species, yielded a false-positive result for the C. concisus strain. In addition to false-positive hippurate test results for the strains that were reliably identified as species other than C. jejuni by molecular tests, false-negative results were also reported that brought the reliability of the test into question, especially for epidemiological investigations that are critical to intervene appropriate preventive strategies [ 31 , 32 ]. Nalidixic acid susceptibility testing was one of the historical phenotypical methods that was used for species-level identification of Campylobacter species.…”

Section: Discussionmentioning

confidence: 99%

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Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea

Teksoy,

Ilktac,

Ongen

2023

Healthcare

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Campylobacter is one of the most commonly reported foodborne bacteria worldwide. Although Campylobacter jejuni and Campylobacter coli have been reported to be responsible for the great majority of campylobacteriosis, the burden of infections by species other than C. jejuni and C. coli have been increasing as a result of a transition to diagnostic test methods that enable the isolation of emerging species. The aim of the present study was to recover C. jejuni, C. coli, and emerging species from the stool samples of 500 patients with gastroenteritis and 100 healthy subjects via the use of a filtration method and culture techniques using Butzler agar and mCCDA under a microaerobic or hydrogen-enriched atmosphere, identify the species by multiplex PCR methods and assess the significance of emerging species in enteric diseases. Thirty-one (6.2%) Campylobacter spp. were isolated from the stool samples of diarrheic patients but none from healthy individuals. Of 31 isolates, 21 (67.8%), nine (29%), and one (3.2%) were identified as C. jejuni, C. coli, and Campylobacter concisus by multiplex PCR, respectively. The filtration method was superior to the culture technique using mCCDA under a microaerobic atmosphere. C. concisus was evaluated as the etiology of gastroenteritis as a result of laboratory and clinical evaluations. The present study was the first to indicate that emerging Campylobacter species are rarely detected and C. concisus is linked to acute gastroenteritis in Turkey where additional studies are warranted to clarify the significance of emerging species in gastroenteritis.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea

Teksoy,

Ilktac,

Ongen

2023

Healthcare

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“…One hundred and fifty‐eight C. jejuni strains isolated from stool samples of patients between 2004 and 2010 were included in the study. Strains were identified to genus and species level by PCR as described in our previous study .…”

Section: Methodsmentioning

confidence: 99%

Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

İlktaç

Öngen

Humphrey

et al. 2019

APMIS

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Campylobacteriosis is one of the most frequently reported zoonoses worldwide. The well‐documented increase in the ciprofloxacin resistance has increased the importance of rapid detection of the resistance. The incidence of ciprofloxacin resistance was investigated using real‐time PCR. Identification of one hundred and fifty‐eight strains was performed by PCR. Minimum inhibitory concentration (MIC) of ciprofloxacin was determined by Epsilometer test. Following the confirmation of the efficiencies of singleplex real‐time PCR methods using two different probes, a cytosine to thymine point mutation at codon 86 was detected by allelic discrimination. Of the 158 strains, 114 (72.2%) were determined to be resistant to ciprofloxacin. The MIC50 and the MIC90 of ciprofloxacin were found to be 8 and ≥32 mg/L, respectively. By real‐time PCR, the presence of the mutation was confirmed in all, but one, resistant strains and the absence of the mutation was demonstrated in all, but one, susceptible strains. The rate of resistance is high among C. jejuni strains and ciprofloxacin should not be used in the treatment of such infections in Turkey. A cytosine to thymine mutation is the most frequently detected mechanism for the resistance. Real‐time PCR can be used for the quick screening of the resistance.

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“…40,52 Although there is scant data about Campylobacter species isolated from children in the Middle East, multiple resistant strains of Campylobacter from human sources have been documented in many recent publications from the Middle East regions. 57,64,65 The observed resistance patterns in Campylobacter in the Middle East may be linked to several factors, with self-medication and antimicrobial abuse remaining a significant contributing factor that create such issue. [66][67][68] This circumstance poses a significant challenge in the treatment of Campylobacter infections as some strains have become resistant to multiple antibiotics, limiting the effectiveness of available treatment options.…”

Section: Antimicrobial Resistance Of C Jejuni In Pediatric Papulation...mentioning

confidence: 99%

Campylobacter infections in Middle Eastern children: Review article

Al-Haddad,

Matran,

Omer

2024

IJMMTD

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() infections among children have become an alarming challenge for developed and developing countries. This narrative review highlights the concerning situation of and its antimicrobial resistance among Middle Eastern children. The review targeted studies on infections in the Middle East, focusing on those publications that were conducted within the past 14 years. The selected publications were sourced from the Google Scholar and PubMed databases. There was scanty published data addressing compylobacteriosis and its antimicrobial profile specifically among Middle Eastern children. Although there has been international concern about the role of and in causing childhood diarrhea, many regions in the Middle East do not appear to share the same level of concern. Furthermore, the complex interplay of socio-demographic and environmental elements that may influence infections, coupled with the globally rising concern over antibiotic resistance in spp (particularly multidrug-resistant strains), raises significant concerns in this region. Moreover, the presence of co-infections with , known to worsen disease severity, could exacerbate the issue and place a greater burden on healthcare systems. Additionally, the low awareness and insufficient prioritization of this public health issue, compounded by political conflicts in parts of the Middle East, present multiple challenges for children in several regions of the Middle East. These challenges, along with the consequences of late sequelae from infection, put many children in this region at significant risk. To effectively tackle these challenges, critical targeted interventions must be implemented. These interventions include conducting comprehensive epidemiological studies to understand the burden of in Middle Eastern children, characterizing the antibiotic susceptibility patterns of prevalent strains, updating healthcare practices to align with prevailing resistance profiles, adopting appropriate antibiotic prescription policies, and implementing robust infection control measures in this specific population.

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Molecular Typing of Campylobacter jejuni and Campylobacter coli of Human Strains Isolated in Turkey Over an Eight-Year Period

Cited by 5 publications

References 0 publications

Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea

Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea

Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

Campylobacter infections in Middle Eastern children: Review article

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