Molecular Study on Foot and Mouth Disease Virus in Beheira Governorate, Egypt during 2014

Ibrahim, Madiha S.; Ahmed, Lamia A.; Kasem, Samy; Hodhod, Ahmed A.

doi:10.5455/ajvs.177714

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…The results of virus isolation on BHK21 cell line in this study revealed cell rounding, aggregation, clustering, and partial sheet detachments within 24-48hrs post infection. These results completely agreed with Ibrahim, et al (2014).…”

Section: Discussionsupporting

confidence: 87%

“…The results of RT-PCR revealed FMD virus serotype O in the examined sample which has been responsible for many recent outbreaks in Egypt as reported by Ibrahim, et al (2014) and also by Aidaros, et al (2016) who found that the most prevalent serotype was (O) in Gharbia and Kaluabiagovernorates during 2014-2015. However, the involvement of other virus strains such as A and SAT2 were previously isolated in Egypt by El-Ashmawy, et al (2013), El-Bayoumy, et al (2014) and Ali, et al (2016).…”

Section: Discussionsupporting

confidence: 54%

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Pathological Studies on Foot and Mouth disease at Kaluobia Governorate

Gab-Allah¹,

El-Mashad²,

Moustafa³

et al. 2018

Benha Veterinary Medical Journal

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The aim of this study was to detect the pathological affections in various body organs in dead animals by foot and mouth disease (FMD) throughout Kaluobia Governorate, Egypt. The study included 882 clinically affected animals showing characteristic signs of foot and mouth disease including, 827 cattle, and 55 buffalo. The age of the examined animals ranged from 30 days to 3 years and they were of both sexes. The total mortality rates were 6.65% in cattle and 54% in buffalos. The most common clinical signs that appeared on examined cases represented by fever, depression, recumbency, lameness, ropy salivation, ptyalism with protrusion of the tongue. Moreover, in young calf's sudden death was the only observed clinical signs. Histopathological examination of both young calves (1-2 months old) and aged calves (12-24 months old), revealed extensive inflammatory and necrotic lesions in the myocardium, lung, liver, kidney, spleen and intestine. Viral isolation from tongue epithelium and molecular identification revealed the presence of FMD virus 'type O'.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 54%

Pathological Studies on Foot and Mouth disease at Kaluobia Governorate

Gab-Allah¹,

El-Mashad²,

Moustafa³

et al. 2018

Benha Veterinary Medical Journal

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“…Differentiating between these two categories of animals is important during serological surveys to detect evidence of infection in vaccinated animals [17]. Lower results of FMD detection were reported by El-Rhman et al [11] and a nearly similar result was reported by Salem et al [18], while higher results were reported by Ibrahim1 et al [19] and Zeedan et al [20] who used FMD-3ABC to distinguish FMDV-infected or carrier animals from vaccinated animals in Egypt.…”

Section: Discussionmentioning

confidence: 92%

Detection of foot-and-mouth disease virus in raw milk in Menofia Governorate and its effect on reproductive hormones and physiochemical properties of milk

et al. 2022

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Background and Aim: Foot-and-mouth disease (FMD) is an extremely contagious viral disease that affects domestic and wild cloven-hoofed animals. In Egypt, FMD has been enzootic since the 1950s and caused great economic losses in cattle and buffalos over the past few years. This study aimed to detect FMD virus (FMDV) in serum and raw milk samples collected from infected and adjacent cattle and buffalos from different localities in El Menofia Governorate, Egypt.Materials and Methods: Blood and milk samples were collected from apparently diseased and adjacent 100 cows and 100 buffalos. Serum samples were prepared and used for the detection of FMDV using a non-structural protein enzyme-linked immunosorbent assay, while real-time reverse transcription-polymerase chain reaction (rRT-PCR) was used for the detection of FMDV in milk samples. Reproductive hormones were estimated using radioimmunoassay kits. Milk constituents were determined by Lactoscan.Results: Of the 200 examined serum samples (100 cows and 100 buffalos), 56% and 44% were seropositive for FMDV non-structural protein antibodies in cattle and buffalo, respectively. Real-time reverse transcription-polymerase chain reaction results confirmed that all examined milk samples collected from seropositive animals were positive for FMDV. Estrogen and progesterone levels in the serum of seropositive and seronegative animals were measured, and FMDV was proven to significantly elevate estrogen and reduce progesterone levels in both non-pregnant and pregnant animals during different stages of pregnancy. The effect of the virus on milk composition and somatic cell count (SCC) was also studied, revealing that FMDV infection significantly decreased the level of milk fat, protein, and lactose but did not significantly affected minerals, pH, and conductivity. Moreover, it significantly increased the SCC. Conclusion:Data recorded in this study indicates a widespread occurrence of FMDV in cattle and buffalo all over Menofia Governorate, Egypt. Infected raw milk is of poor quality and, if put for commercial sale, may have health risks for consumers and play a significant role in spreading the virus. Moreover, FMDV may disturb some reproductive hormones, which could adversely affect cattle and buffalo productivity. Therefore, preventive programs and accurate diagnosis are essential for successful disease control.

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Molecular Detection and Serotyping of Foot and Mouth Disease Virus in Tongue Epithelium Samples

El-Kenawy

Younis

Fakhry

et al. 2017

Mansoura Veterinary Medical Journal

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The aim of the present study is molecular identification and typing of FMDV circulating in Dakahlia governorate in Egypt, 2014. The study was accomplished on pooled nine tongue epithelium samples collected from diseased cattle and buffaloes expected to be infected with FMDV. The diseased animals showed fever , excessive salivation and ulceration on tongue and gum. Conventional reverse transcription polymerase chain reaction (RT-PCR) was used for identification of FMDV using universal primers. Then serotyping of the identified virus was done using RT-PCR for serotype O and A and Real time RT-PCR for serotype SAT2 . FMDV was identified in all the examined nine samples. Five of them were of serotype O, two of serotype A, one of serotype SAT2 and one sample was neither O, A nor SAT2. In conclusion, molecular techniques are sensitive and rapid methods for FMDV detection. In addition, serotypes O, A and SAT2 are circulating in Dakahlia Governorate, Egypt, 2014.

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Molecular Study on Foot and Mouth Disease Virus in Beheira Governorate, Egypt during 2014

Cited by 3 publications

References 24 publications

Pathological Studies on Foot and Mouth disease at Kaluobia Governorate

Pathological Studies on Foot and Mouth disease at Kaluobia Governorate

Detection of foot-and-mouth disease virus in raw milk in Menofia Governorate and its effect on reproductive hormones and physiochemical properties of milk

Molecular Detection and Serotyping of Foot and Mouth Disease Virus in Tongue Epithelium Samples

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