Molecular regulation of steroidogenesis in endocrine Leydig cells

Tremblay, Jacques

doi:10.1016/j.steroids.2015.08.001

Cited by 146 publications

(98 citation statements)

References 166 publications

(196 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tremblay 2015). FLCs, which do not express Sry (Zwingman et al 1994), differentiate in response to Sertoli cell factors that include platelet-derived growth factor A (PDGFA) and in response to Dessert Hedgehog (DHH) and Notch signaling (Tang et al 2008; reviewed in Barsoum and Yao 2006;O'Shaughnessy et al 2006;Svingen and Koopman 2013).…”

Section: The Interstitiummentioning

confidence: 99%

Biology of the Sertoli Cell in the Fetal, Pubertal, and Adult Mammalian Testis

Chojnacka¹,

Zarzycka

Mruk³

2016

Results and Problems in Cell Differentiation

View full text Add to dashboard Cite

A healthy man typically produces between 50 × 10(6) and 200 × 10(6) spermatozoa per day by spermatogenesis; in the absence of Sertoli cells in the male gonad, this individual would be infertile. In the adult testis, Sertoli cells are sustentacular cells that support germ cell development by secreting proteins and other important biomolecules that are essential for germ cell survival and maturation, establishing the blood-testis barrier, and facilitating spermatozoa detachment at spermiation. In the fetal testis, on the other hand, pre-Sertoli cells form the testis cords, the future seminiferous tubules. However, the role of pre-Sertoli cells in this process is much less clear than the function of Sertoli cells in the adult testis. Within this framework, we provide an overview of the biology of the fetal, pubertal, and adult Sertoli cell, highlighting relevant cell biology studies that have expanded our understanding of mammalian spermatogenesis.

show abstract

Section: The Interstitiummentioning

confidence: 99%

Biology of the Sertoli Cell in the Fetal, Pubertal, and Adult Mammalian Testis

Chojnacka¹,

Zarzycka

Mruk³

2016

Results and Problems in Cell Differentiation

View full text Add to dashboard Cite

show abstract

“…Here, we describe a new Leydig cell ablation model based on delivery of Cre recombinase into the testes of mice harboring floxed alleles of Gata4 and Gata6 , two key regulators of steroidogenic cell differentiation and function (Tevosian 2014, Röhrig et al 2015, Tremblay 2015). Gata4 and Gata6 are expressed in fetal/adult Leydig cells (Ketola et al 1999, Ketola et al 2002, Mazaud-Guittot et al 2014) and have been shown to activate the promoters of several steroidogenic genes, including Cyp11a1 and Cyp17a1 (Tremblay & Viger 2001, Jimenez et al 2003, Rahman et al 2004, Sher et al 2007) .…”

Section: Introductionmentioning

confidence: 99%

Probing GATA factor function in mouse Leydig cells via testicular injection of adenoviral vectors

et al. 2017

View full text Add to dashboard Cite

Testicular Leydig cells produce androgens essential for proper male reproductive development and fertility. Here, we describe a new Leydig cell ablation model based on Cre/Lox recombination of mouse Gata4 and Gata6, two genes implicated in the transcriptional regulation of steroidogenesis. The testicular interstitium of adult Gata4flox/flox;Gata6flox/flox mice was injected with adenoviral vectors encoding Cre + GFP (Ad-Cre-IRES-GFP) or GFP alone (Ad-GFP). The vectors efficiently and selectively transduced Leydig cells, as evidenced by GFP reporter expression. Three days after Ad-Cre-IRES-GFP injection, expression of androgen biosynthetic genes (Hsd3b1, Cyp17a1, Hsd17b3) was reduced, whereas expression of another Leydig cell marker, Insl3, was unchanged. Six days after Ad-Cre-IRES-GFP treatment, the testicular interstitium was devoid of Leydig cells, and there was a concomitant loss of all Leydig cell markers. Chromatin condensation, nuclear fragmentation, mitochondrial swelling, and other ultrastructural changes were evident in the degenerating Leydig cells. Liquid chromatography-tandem mass spectrometry demonstrated reduced levels of androstenedione and testosterone in testes from mice injected with Ad-Cre-IRES-GFP. Late effects of treatment included testicular atrophy, infertility, and the accumulation of lymphoid cells in the testicular interstitium. We conclude that adenoviral-mediated gene delivery is an expeditious way to probe Leydig cell function in vivo. Our findings reinforce the notion that GATA factors are key regulators of steroidogenesis and testicular somatic cell survival.

show abstract

“…patients caused by blockages at one or more sites along the hypothalamic-pituitary-testicular (HPT) axis (12,13). Testicular level is due to dysfunction of Leydig cells because of pro-inflammatory cytokines, such as TNF-α, IL-1, and interleukin 6 (IL-6), which inhibit testicular Leydig cell steroidogenesis at the level of gene expression of different steroidogenic enzymes induced by CKD and HD (14), LH reduced clearance, as well as hyperprolactinemia caused by reduced clearance of PRL (12).…”

Section: Discussionmentioning

confidence: 99%

Systemic Inflammation and Seminal Parameters in Chronic Hemodialysis Patients

2018

View full text Add to dashboard Cite

Objectives: We proposed to investigate the possible effect and association of systemic inflammation (SI) and seminal parameter indicators in chronic hemodialysis patients. Methods: This was a case-control study. All the participants were subjected to a spermiogram with calculation of fertility index (FI), serum C-reactive protein (CRP) level, seminal transferrin (ST) level, as well as evaluation of the hormonal profile (HP). The sample consisting of 60 men (cases) undergoing hemodialysis for more than 6 months was subdivided into 3 groups: group 1 (n = 30, with inflammation, CRP > 5 mg/L), group 2 (n = 30, without inflammation, CRP ≤ 5 mg/L), and group 3 (n = 30, healthy men, CRP ≤ 1). Results:Age was similar in the 3 groups (P = 0.43). FI, testosterone total (TT) and ST levels were significantly lower in the case groups than in the control group (P < 0.001). Follicle stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) levels were significantly higher in the case groups than in the control group (P < 0.001). Between the subgroups of cases (groups 1 and 2), the inflammatory factor alone does not seem to interfere with the FI, HP, and ST level (P > 0.05). However, it significantly interfered with the FI, HP, and ST level when compared between the case groups and the control group (P < 0.001). No correlation was observed between SI and analyzed parameters (P > 0.05). Conclusions:The results suggest that the SI alone has no effect and is not associated with the FI or ST level in a patient undergoing chronic hemodialysis.

show abstract

Molecular regulation of steroidogenesis in endocrine Leydig cells

Cited by 146 publications

References 166 publications

Biology of the Sertoli Cell in the Fetal, Pubertal, and Adult Mammalian Testis

Biology of the Sertoli Cell in the Fetal, Pubertal, and Adult Mammalian Testis

Probing GATA factor function in mouse Leydig cells via testicular injection of adenoviral vectors

Systemic Inflammation and Seminal Parameters in Chronic Hemodialysis Patients

Contact Info

Product

Resources

About