Molecular Phylogenetic Analyses of Biological Control Strains of Trichoderma harzianum and Other Biotypes of Trichoderma spp. Associated with Mushroom Green Mold

Abstract

A polymerase chain reaction-amplified DNA containing the internal transcribed spacer (ITS)-1, 5.8S, and ITS-2 regions of the nuclear ribosomal DNA transcriptional unit was sequenced for 81 isolates of Trichoderma spp. associated with mushroom culture or used for biological control of plant pathogens. Phylogenetic analyses revealed that the biocontrol isolates were more closely related to an isolate of T. harzianum biotype 1 (Th1) than to the aggressive biotypes 2 and 4. Th1 has been isolated from mushroom compost but is not the cause of widespread green mold epidemics that have occurred during the last 12 years in Europe and North America. Three isolates of T. harzianum obtained from shiitake (Lentinula edodes; Shi1B and S3-96) and maitake (Grifola frondosa; Mai1) substrates were placed within the biocontrol group. We also found evidence suggesting that some isolates of T. harzianum originally identified as Th4 from Pennsylvania are more closely related to Th2 from Europe. Finally, considering the wide range in sequence distribution of our samples, we propose that the consensus sequence found in this investigation be used as the reference sequence for further studies involving the identification and taxonomy of T. harzianum.

Molecular Characterization and Identification of Biocontrol Isolates of Trichoderma spp

“…Molecular studies have confirmed the distribution of T. harzianum in the same four Seaby's biotypes, of which Th1 and Th3 seem to be nonpathogenic to mushrooms (28). Phylogenetic analysis based on ITS sequences (27) has revealed that Th1 is the most recent ancestor for aggressive mushroom colonizers which were included in Th2 (26) and Th4 (28) of T. harzianum. Th3 is coincident with T. atroviride Karsten, a previously described species with similarities in colony character to T. harzianum (21).…”

Effect of <i>Trichoderma harzianum</i> in Combination with Fungicides in Controlling Gray Mould Disease (<i>Botrytis cinerea</i>) of Strawberry

“…Trichoderma harzianum isolate (Jn14) was identified based on RAPD analysis and the sequence of (ITS) [18], and formulated as wettable powder (WP) on Talc. The isolate was grown on 90 mm PDA medium plates for 10 days.…”

Eksplorasi Dan Identifikasi Trichoderma Endofitik Pada Pisang

“…Analisis sekuens internal transcribed spacer region 1 dan 2 (ITS1 dan ITS2) pada DNA ribosomal dapat membantu untuk deskripsi dan karakterisasi spesies pada genus Trichoderma (Ospina-Giraldo et al, 1999;Jeewon et al, 2013). Seluruh analisis tersebut menggunakan amplifikasi PCR karena dapat menghasilkan produk yang akurat, sehingga dapat digunakan dalam melakukan diagnosis (McPherson & Moller, 2006 (Regina et al, 2003).…”