Molecular Interactions between Saliva and Dental Composites Resins: A Way Forward

Șaramet, Veaceslav; Meleşcanu-Imre, Marina; Țâncu, Ana Maria Cristina; Albu, Crenguta; Totan, Alexandra Ripszky; Pantea, Mihaela

doi:10.3390/ma14102537

Cited by 11 publications

(11 citation statements)

References 71 publications

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“…In particular, the cells were not placed in direct contact with the CRs but were treated with the soaking extracts obtained from the immersion of dental CR disks in the culture medium for 24 h and 14 days. Indeed, this experimental method simulated a condition closer to the oral milieu [ 2 ], where the oral soft tissues could be negatively affected by the residual monomers derived from the various CR components following saliva interaction [ 37 ]; this resulted in the most commonly used approach in the literature at the moment [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

et al. 2021

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The aim of the study was to evaluate the cytotoxic and genotoxic potential of five commercially available dental composite resins (CRs), investigating the effect of their quantifiable bisphenol-A-glycidyl-methacrylate (Bis-GMA) and/or triethylene glycol dimethacrylate (TEGDMA) release. Experiments were performed using the method of soaking extracts, which were derived from the immersion of the following CRs in the culture medium: Clearfil-Majesty-ES-2, GrandioSO, and Enamel-plus-HRi (Bis-GMA-based); Enamel-BioFunction and VenusDiamond (Bis-GMA-free). Human Gingival Fibroblasts (hGDFs) were employed as the cellular model to mimic in vitro the oral cavity milieu, where CRs simultaneously release various components. Cell metabolic activity, oxidative stress, and genotoxicity were used as cellular outcomes. Results showed that only VenusDiamond and Enamel-plus-HRi significantly affected the hGDF cell metabolic activity. In accordance with this, although no CR-derived extract induced a significantly detectable oxidative stress, only VenusDiamond and Enamel-plus-HRi induced significant genotoxicity. Our findings showed, for the CRs employed, a cytotoxic and genotoxic potential that did not seem to depend only on the actual Bis-GMA or TEGDMA content. Enamel-BioFunction appeared optimal in terms of cytotoxicity, and similar findings were observed for Clearfil-Majesty-ES-2 despite their different Bis-GMA/TEGDMA release patterns. This suggested that simply excluding one specific monomer from the CR formulation might not steadily turn out as a successful approach for improving their biocompatibility.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

et al. 2021

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“…The evaluation models should be pertinent for the in vivo environment to assure that the dental materials will withstand in the oral environment without any depreciation [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Behavior of Two CAD/CAM Fiber-Reinforced Composite Dental Materials by Immersion Tests

et al. 2021

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Fiber-reinforced composites are used as restorative materials for prosthetic oral rehabilitation. Gastroesophageal reflux disease (GERD) is an accustomed affection with various oral manifestations. This study aimed to evaluate the behavior of two high-performance CAD/CAM milled reinforced composites (Trinia™, TriLor) in artificial saliva at different pH levels through immersion tests, and to determine if changes in mass or surface morphology at variable pH, specific for patients affected by GERD, appear. After investigating the elemental composition and surface morphology, the specimens were immersed in Carter Brugirard artificial saliva for 21 days at different pH values (5.7, 7.6, and varying the pH from 5.7 to 3). The values of the weighed masses during the immersion tests were statistically processed in terms of mean and standard deviation. Results suggested that irrespective of the medium pH, the two composites presented a similar mass variation in the range of −0.18 (±0.01)–1.82 (±0.02) mg after immersion, suggesting their stability when in contact with artificial saliva, an aspect which was also highlighted by scanning electron microscope (SEM) analysis performed on the immersed surfaces. Novel composite biomaterials can be a proper alternative for metal alloys used for prosthetic frameworks in patients suffering from GERD.

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“…This approach reflects the properties of dental composite, as well as certain aspects of the potential side-effects caused by the patient’s pathology. Additionally, the subject addressed has a practical connotation, since dental practitioners should be constantly informed and aware about the properties of dental materials and should personalize the selection of restoration materials in accordance with the complexity of each clinical case [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Dental Resin-Based Composites in Leukemia Saliva: An In Vitro Analysis

et al. 2021

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Background: The aim was to analyze, in vitro, four resin based composite systems (RBCs) immersed in saliva of leukemia patients before starting chemotherapy regiments. Material and methods: Saliva was collected from 20 patients (4 healthy patients, 16 leukemia patients). Resin disks were made for each RBC and were immersed in the acute leukemia (acute lymphocytic (ALL), acute myeloid (AML)), chronic leukemia (chronic lymphocytic (CLL), chronic myeloid (CML)), Artificial saliva and Control environment, and maintained for seven days. At the end of the experiment, the characteristics and the effective response of saliva from the studied salivas’ on RBCs was assessed using water sorption, water solubility, residual monomer and scanning electron microscopy (SEM). Data analysis was performed and a p-value under 0.05 was considered statistically significant. Results: The behaviour of RBCs in different immersion environments varies according to the characteristics of the RBCs. RBCs with a higher filler ratio have a lower water sorption. The solubility is also deteriorated by the types of organic matrix and filler; the results of solubility being inversely proportional on the scale of negative values compared to sorption values. Chromatograms of residual monomers showed the highest amount of unreacted monomers in ALL and AML, and the Control and artificial saliva environments had the smallest residual monomer peaks. Because of the low number of differences between the experimental conditions, we further considered that there were no important statistical differences between experimental conditions and analysed them as a single group. Conclusion: The influence of saliva on RBCs depends on the type of leukemia; acute leukemia influenced the most RBCs by changing their properties compared to chronic leukemia.

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Molecular Interactions between Saliva and Dental Composites Resins: A Way Forward

Cited by 11 publications

References 71 publications

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

Evaluation of the Behavior of Two CAD/CAM Fiber-Reinforced Composite Dental Materials by Immersion Tests

Characteristics of Dental Resin-Based Composites in Leukemia Saliva: An In Vitro Analysis

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