Characteristics of Dental Resin-Based Composites in Leukemia Saliva: An In Vitro Analysis

Abstract

Background: The aim was to analyze, in vitro, four resin based composite systems (RBCs) immersed in saliva of leukemia patients before starting chemotherapy regiments. Material and methods: Saliva was collected from 20 patients (4 healthy patients, 16 leukemia patients). Resin disks were made for each RBC and were immersed in the acute leukemia (acute lymphocytic (ALL), acute myeloid (AML)), chronic leukemia (chronic lymphocytic (CLL), chronic myeloid (CML)), Artificial saliva and Control environment, and maintained for seven days. At the end of the experiment, the characteristics and the effective response of saliva from the studied salivas’ on RBCs was assessed using water sorption, water solubility, residual monomer and scanning electron microscopy (SEM). Data analysis was performed and a p-value under 0.05 was considered statistically significant. Results: The behaviour of RBCs in different immersion environments varies according to the characteristics of the RBCs. RBCs with a higher filler ratio have a lower water sorption. The solubility is also deteriorated by the types of organic matrix and filler; the results of solubility being inversely proportional on the scale of negative values compared to sorption values. Chromatograms of residual monomers showed the highest amount of unreacted monomers in ALL and AML, and the Control and artificial saliva environments had the smallest residual monomer peaks. Because of the low number of differences between the experimental conditions, we further considered that there were no important statistical differences between experimental conditions and analysed them as a single group. Conclusion: The influence of saliva on RBCs depends on the type of leukemia; acute leukemia influenced the most RBCs by changing their properties compared to chronic leukemia.

Structural Changes in Resin-Based Composites in Saliva of Patients with Leukemia before Starting Chemotherapeutic Regimen

“…This discoloration can be attributed to the degrees of hydrophilicity and absorption of fluids found in leukemia saliva. As seen in one of our previous studies, acute leukemia influenced more RBCs by changing their properties compared to chronic leukemia [41].…”

Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to Streptococcus mutans and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study

“…The veneer–cement-adhesive–tooth (enamel) interface revealed a homogeneous and uniformly thick layer of Variolink Esthetic DC adhesive cement. At the boundary between the veneer and the adhesive cement, there were areas with microcracks due to the contraction of the cement during light curing ( Figure 3 b), but the contact was intimate, without gaps, without areas of separation of the two materials, emphasizing a tight adhesion of cement to ceramics [ 35 ]. The evaluation of the junction between the adhesive cement and the tooth (enamel) showed very good adhesion, an adhesive system of uniform thickness, and a weakly represented but present hybrid layer ( Figure 3 c).…”