Molecular identification of the causative agent of human strongyloidiasis acquired in Tanzania: Dispersal and diversity of Strongyloides spp. and their hosts

Hasegawa, Hideo; Sato, Hiroshi; Fujita, Shiho; Nguema, Pierre Philippe Mbehang; Nobusue, Kenichi; Miyagi, Kei; Kooriyama, Takanori; Takenoshita, Yuji; Noda, Shohei; Satô, Akiko; Morimoto, Azusa; Ikeda, Yatsukaho; Nishida, Toshisada

doi:10.1016/j.parint.2010.05.007

Cited by 83 publications

(120 citation statements)

References 21 publications

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“…10 also showed that S . stercoralis isolates from humans, chimpanzees, and dogs can be grouped into dog-parasitic and primate-parasitic clades using the mitochondrial Cox1 gene as a genetic marker from human (n = 5), chimpanzee (n = 2), and dog isolates (n = 2) 10 . The first report to determine worm genotypes at a population level were conducted in Cambodia by Schar et al .…”

Section: Introductionmentioning

confidence: 93%

A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

Nagayasu

Aung²,

Hortiwakul

et al. 2017

Sci Rep

108

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Humans and dogs are the two major hosts of Strongyloides stercoralis, an intestinal parasitic nematode. To better understand the phylogenetic relationships among S. stercoralis isolates infecting humans and dogs and to assess the zoonotic potential of this parasite, we analyzed mitochondrial Cox1, nuclear 18S rDNA, 28S rDNA, and a major sperm protein domain-containing protein genes. Overall, our analyses indicated the presence of two distinct lineages of S. stercoralis (referred to as type A and type B). While type A parasites were isolated both from humans and dogs in different countries, type B parasites were found exclusively in dogs, indicating that the type B has not adapted to infect humans. These epidemiological data, together with the close phylogenetic relationship of S. stercoralis with S. procyonis, a Strongyloides parasite of raccoons, possibly indicates that S. stercoralis originally evolved as a canid parasite, and later spread into humans. The inability to infect humans might be an ancestral character of this species and the type B might be surmised to be an origin population from which human-infecting strains are derived.

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Section: Introductionmentioning

confidence: 93%

A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

Nagayasu

Aung²,

Hortiwakul

et al. 2017

Sci Rep

108

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show abstract

“…The partial cox-1 gene of parasite mtDNA was amplified by a primer pair of StrCoxAfrF (5'-GTGGTTTTGGTAAT TGAATGGTT-3') and MH28R (5'-CTAACTACATAAT AAGTATCATG-3') described by Hasegawa et al (2010), but a different cycling protocol was used as follows: 2 min at 94˚C, then 40 cycles at 94˚C for 30 sec, 51˚C for 45 sec, and 72˚C for 1 min, followed by a final extension at 72˚C for 7 min. Amplicons were sequenced after genomic cloning as detailed above.…”

Section: Dna Extraction Polymerase Chain Reaction (Pcr) and Sequencingmentioning

confidence: 99%

“…All these 16 sequences were absolutely identical to each other, and to a deposited 18S rDNA sequence of Strongyloides fuelleborni from a Japanese mammalogist visiting Tanzania (DDBJ/EMBL/GenBank accession no. AB453320; Hasegawa et al, 2010). Identities of the newly obtained sequence (accession nos.…”

Section: Prevalence Of Gastrointestinal Parasites In Gorillas and Hummentioning

confidence: 99%

Prevalence and genetic diversity of Oesophagostomum stephanostomum in wild lowland gorillas at Moukalaba-Doudou National Park, Gabon

et al. 2014

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Using a sedimentation method, the prevalence of the nodular worm Oesophagostomum stephanostomum (Nematoda: Strongylida) in western lowland gorillas at Moukalaba-Doudou National Park (MDNP), Gabon, was determined in fecal samples collected between January 2007 and October 2011, along with their coprocultures. Concurrently, possible zoonotic Oesophagostomum infections in villagers living near MDNP were assessed from their fecal samples collected during October and November of 2011. In the gorillas, strongylid (Oesophagostomum and/or hookworm) eggs were found in 47 of 235 fecal samples (20.0 %) and Oesophagostomum larvae were detected in 101 of 229 coprocultures (44.1 %). In the villagers, strongylid eggs were found in 9 of 71 fecal samples (12.7 %), but no Oesophagostomum larvae were detected in coprocultures. The internal transcribed spacer (ITS) region of ribosomal RNA gene (rDNA) and cytochrome c oxidase subunit-1 (cox-1) region of mitochondrial DNA (mtDNA) of coprocultured Oesophagostomum larvae were amplified using parasite DNA extracted from 7–25 larvae/sample, cloned into Escherichia coli, and sequenced. Sequenced rDNA contained 353/354-bp long ITS1, 151-bp long 5.8S rDNA, and 227-bp long ITS2. Parts of clones showed variations at 1–3 bases in the ITS1 region at a frequency of 24/68 (35.3 %) and at 1–2 bases in the ITS2 region at a frequency of 7/68 (10.3 %), whereas the 5.8S rDNA was essentially identical. Sequenced cox-1 gene of the parasites, 849 bp in length, showed a higher number of nucleotide variations, mainly at the third nucleotide position of the codon. The majority of clones (27/41 (65.9 %)) had an identical amino acid sequence. These results suggest that at MDNP, Gabon, only a single population of O. stephanostomum with a degree of genetic diversity is prevalent in western lowland gorillas, without zoonotic complication in local inhabitants. The possible genetic variations in the ITS region of rDNA and cox-1gene of mtDNA presented here may be valuable when only a limited amount of material is available for the molecular species diagnosis of O. stephanostomum.

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“…The amplicon of 18S gene was 1.7 kb long, comprising the hypervariable region IV (HVR-IV) of 18S (Hasegawa et al, 2010). The amplicon of the partial Cox1 gene was 1 kb long.…”

Section: Dna Sequence Analysismentioning

confidence: 99%

Enterobius(Enterobius)shriverin. sp. (Nematoda: Oxyuridae: Enterobiinae) fromMacaca fascicularisRaffles, 1821 (Primates: Cercopithecidae: Cercopithecinae) and Three Other Cercopitheciid Primate Species: With Additional Information onEnterobius(Enterobius)macaciYen, 1973

Bolette

Cui

Rogers

2016

Comparative Parasitology

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Molecular identification of the causative agent of human strongyloidiasis acquired in Tanzania: Dispersal and diversity of Strongyloides spp. and their hosts

Cited by 83 publications

References 21 publications

A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

Prevalence and genetic diversity of Oesophagostomum stephanostomum in wild lowland gorillas at Moukalaba-Doudou National Park, Gabon

Enterobius(Enterobius)shriverin. sp. (Nematoda: Oxyuridae: Enterobiinae) fromMacaca fascicularisRaffles, 1821 (Primates: Cercopithecidae: Cercopithecinae) and Three Other Cercopitheciid Primate Species: With Additional Information onEnterobius(Enterobius)macaciYen, 1973

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