A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

Nagayasu, Eiji; Aung, Myo Pa Pa Thet Hnin Htwe; Hortiwakul, Thanaporn; Hino, Akinori; Tanaka, Teruhisa; Higashiarakawa, Miwa; Olia, Alex; Taniguchi, Tomoyo; Win, Soe Moe Thu; Ōhashi, Isao; Odongo-Aginya, Emmanuel Igwaro; Aye, Khin Myo; Mon, Mon; Win, Kyu Kyu; Ota, Kei; Torisu, Yukari; Panthuwong, Siripen; Kimura, Eizen; Palacpac, Nirianne; Kikuchi, Taisei; Hirata, Tetsuya; Torisu, Shidow; Hisaeda, Hajime; Horii, Toshihiro; Fujita, Jiro; Htike, Wah Win; Maruyama, Haruhiko

doi:10.1038/s41598-017-05049-x

Cited by 65 publications

(121 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are two regions of the S. stercoralis nuclear and mitochondrial genome that are considered to be conserved within the Strongyloides genus and can be used as markers for molecular typing of S. stercoralis . Hyper-variable regions (HVR) I and IV of the small Subunit ( SSU ) 18s ribosomal DNA and the cytochrome c-oxidase subunit 1 ( cox 1) gene of the mitochondrial DNA (mtDNA) have been widely used to study relationships between S. stercoralis from different hosts and different geographic locations (Hasegawa et al, 2009, Hasegawa et al, 2010, Jaleta et al, 2017, Nagayasu et al, 2017, Basso et al, 2018). Based on genetic analysis of these genetic loci, it has been recently found that there are two genetically different S. stercoralis strains, one is dog and human infective, and the other is dog specific.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of classic and cryptic Strongyloides genotypes by deep amplicon sequencing: A preliminary survey of dog and human specimens collected from remote Australian communities

Beknazarova

Barratt

Bradbury

et al. 2019

Preprint

View full text Add to dashboard Cite

17Strongyloidiasis is a neglected disease caused by the human infective nematodes 18 Strongyloides stercoralis, Strongyloides fuelleborni subsp. fuelleborni and Strongyloides 19 fuelleborni subsp. kellyi. The zoonotic potential of S. stercoralis and the potential role of dogs 20 in the maintenance of strongyloidiasis transmission has been a topic of interest and discussion 21 for many years. In Australia, strongyloidiasis is prevalent in remote socioeconomically 22 disadvantaged communities in the north of the continent. Being an isolated continent that has 23 been separated from other regions for a long geological period, knowledge on the diversity of 24 Australian Strongyloides genotypes is an important addition to our understanding of the genetic 25 diversity within the genus. Using PCR enrichment combined with Illumina sequencing 26 technology, we sequenced the Strongyloides SSU 18S rDNA hyper-variable I and hyper-27 variable IV regions using Strongyloides-specific primers, and a fragment of the mtDNA cox128 gene using primers that are broadly specific for Strongyloides sp. and hookworms. These loci 29 were amplified from DNA extracted from human and dog faeces, and one human sputum 30 sample. Using this approach, we confirm for the first time that potentially zoonotic S.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Based on genetic analysis of these genetic loci, it has been recently found that there are two genetically different S. stercoralis strains, one is dog and human infective, and the other is dog specific. These data were collected from dogs and humans in Cambodia and Japan (Jaleta et al, 2017, Nagayasu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Detection of classic and cryptic Strongyloides genotypes by deep amplicon sequencing: A preliminary survey of dog and human specimens collected from remote Australian communities

Beknazarova

Barratt

Bradbury

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Pairwise difference in nucleotides sequence of cox1 gene among isolates of S. stercoralis from humans, apes and dogs was less than 4%, regardless of the host and locality of the isolates [21]. The average pairwise distances of cox1 nucleotide sequences of S. stercoralis, isolated from human and dog collected mainly from Myanmar, were 3.6% [23].…”

Section: Discussionmentioning

confidence: 90%

“…In mentioned study, two genetically different populations of Strongyloides spp.were found in dogs, one of which that the majority of the worms belonged, appeared to be restricted to dogs; the other population was shared with humans [24]. In another study, using nuclear and mitochondrial markers, phylogenetic relationships among S. stercoralis isolates from several human and dog populations in multiple countries of East Asia were examined [23]. Accordingly, two distinct lineage of S. stercoralis were present, referring to as type A parasites isolated both from humans and dogs, and type B parasites founding exclusively in dogs and not adapted to infect humans.…”

Section: Discussionmentioning

confidence: 98%

Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1)

Tehrani

Sharifdini²,

Zahabiun

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Due to the similarity of Strongyloides stercoralis with free-living nematodes of Rhabditis species they might be miss-diagnosed with each other in microscopical examination of stool samples. The aim of this study was molecular characterization and differentiation of human derived isolates of S. stercoralis and Rhabditis species based on the mitochondrial gene of cytochrome c oxidase subunit 1 ( cox1 ) amplification. Methods: Using parasitological methods, ten isolates of S. stercoralis and three isolates of Rhabditis spp. were obtained from fresh stool samples of patients and the genomic DNA of the samples were extracted. PCR amplification of cox1 gene was carried out for all the isolates and the products were sequenced. Results: The phylogenetic analysis illustrated that S. stercoralis and Rhabditis spp. isolates were placed in two distinguishable separate clades. Interspecies genetic variation between isolates of S. stercoralis and Rhabditis spp . were ranged from 13.5 to 14.5%. Conclusions: Cox1 gene was a suitable marker for discrimination of S. stercoralis from Rhabditis spp. retrieved from human in the current study. The availability of gene sequence information will be helpful in the future development and validation of discriminatory PCR-based assays of these nematodes.

show abstract

“…The phylogenetic relationships inferred using both nuclear 18S rDNA HVR-IV and mitochondrial cox1 sequences were basically similar suggesting strong separation of human-dog and dog-specific strains of S. stercoralis. Nagayasu et al [17] also reported the existence of two genetically distinct lineages and clades of S. stercoralis isolated from dogs and humans in Japan and Myanmar using a genotyping scheme similar to that of Jaleta et al [15••]. Several S. stercoralis larvae with different HVR-I and IV genotypes were identified from the same dogs, and there was no evidence of crossing among larvae with different genotypes [15••].…”

Section: Host Specificity Genetic Diversity and Zoonosesmentioning

confidence: 99%

Advances in the Molecular and Cellular Biology of Strongyloides spp.

Jaleta

Lok

2019

Curr Trop Med Rep

View full text Add to dashboard Cite

Purpose of Review This paper constitutes an update of recent studies on the general biology, molecular genetics, and cellular biology of Strongyloides spp. and related parasitic nematodes. Recent Findings Increasingly, human strongyloidiasis is considered the most neglected of neglected tropical diseases. Despite this, the last 5 years has seen remarkable advances in the molecular biology of Strongyloides spp. Genome sequences for S. stercoralis, S. ratti, S. venezuelensis, S. papillosus, and the related parasite Parastrongyloides trichosuri were created, annotated, and analyzed. These genomic resources, along with a practical transgenesis platform for Strongyloides spp., aided a major achievement, the advent of targeted mutagenesis via CRISPR/Cas9 in S. stercoralis and S. ratti. The genome sequences have also enabled significant molecular epidemiologic and phylogenetic findings on human strongyloidiasis, including the first genetic evidence of zoonotic transmission of S. stercoralis between dogs and humans. Studies of molecular signaling pathways identified the nuclear receptor Ss-DAF-12 as one that can be manipulated in the parasite by exogenous application of its steroid ligands. The chemotherapeutic implications of this were unscored by a study in which a Ss-DAF-12 ligand suppressed autoinfection by S. stercoralis in a new murine model of human strongyloidiasis. Summary Seminal advances in genomics of Strongyloides spp. have transformed research into strongyloidiasis, facilitating fundamental phylogenetic and epidemiologic studies and aiding the deployment of CRISPR/Cas9 gene disruption and editing as functional genomic tools in Strongyloides spp. Studies of Ss-DAF-12 signaling in S. stercoralis demonstrated the potential of this pathway as a novel chemotherapeutic target in parasitic nematodes.

show abstract

A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

Cited by 65 publications

References 30 publications

Detection of classic and cryptic Strongyloides genotypes by deep amplicon sequencing: A preliminary survey of dog and human specimens collected from remote Australian communities

Detection of classic and cryptic Strongyloides genotypes by deep amplicon sequencing: A preliminary survey of dog and human specimens collected from remote Australian communities

Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1)

Advances in the Molecular and Cellular Biology of Strongyloides spp.

Contact Info

Product

Resources

About