Molecular identification of multi drug resistant bacteria from urinary tract infected urine samples

Kumar, M.S.; Das, Aradhana

doi:10.1016/j.micpath.2016.06.029

Cited by 32 publications

(9 citation statements)

References 42 publications

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“…These bacteria include Enterobacter agglomerans, Proteus spp., Pseudomonas aeruginosa, Klebsiella spp., Acinetobacter baumanni, and Serratia marscesens. This is similar to the report of Kumar et al 33 That isolated multiple drug resistant Proteus sp., Pseudomonas sp., and Enterobacter sp. from urine samples of UTI patients.…”

Section: Discussionsupporting

confidence: 92%

“…The concentrations of the flavonoids in the extract was extrapolated from standard calibration curve and expressed as milligram rutin equivalent per g of extract (mg RE/g extract). [31][32][33]…”

Section: Estimation Of Total Flavonoid Concentrationmentioning

confidence: 99%

“…The whole solution was allowed to settle and the precipitate was collected, washed with dilute ammonium hydroxide and then filtered; the residue being the alkaloid was dried and weighed to a constant mass. [31][32][33][34][35]…”

Section: Detection Of Alkaloid Contentmentioning

confidence: 99%

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Comparative study between the effect of Parkia Biglobosa (JACQ) benth and conventional antibiotics against multiple antibiotic resistant uropathogenic bacteria (MARUB)

Osuntokun¹,

Jemilaiye²,

Ar³

2018

MOJBB

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The purpose of this research work is to compare the effect of convectional antibiotics with medicinal plant (Parkia biglobosa (Jacq.)Benth) against multiple antibiotics resistant Uropathogenic bacteria isolates.(MARUB), which was isolated from basically two hospital in Ondo State, Nigeria. The two hospitals are Gani Fawehinmi Diagnostic center, Ondo and Federal Medical Center, Owo between July, 2017 and October, 2017. fifty two (52) Gram negative bacteria isolated from urine of UTIs patient at the Gani Fawehinmi Medical Diagnostic Centre, Ondo and Federal Medical Centre, Owo were screened for antibiotic resistance assay, The test isolates were identified, characterized with Microbact™ 24E test strip. it was observed that Twenty (20) bacterial isolates were resistant to at least 76.9% (10 out of 13) of the antibiotics tested, showing susceptibility to the carbapenems and piperacillin/Tazobactam. Five (5) isolates were also susceptible to ofloxacin though with narrower diameter zones of inhibition Parkia biglobosa (Jacq.) Benth s a plant used in traditional medicine for the treatment of various diseases in Nigeria. In order to microbiologically appraise some of the traditional and ethno medical uses of Parkia biglobosa, this study aimed at evaluating and compare the diameter zone of inhibition (antibacterial activities) of Parkia biglobosa leaf and stem bark extracts and conventional antibiotic against multiple antibiotic resistant Uropathogenic bacteria (MARUB). The Parkia biglobosa extracts shows zone of inhibition against Enterobacter agglomerans, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella Subsp. 3B, Serratia marcsesens, Citrobacter freundii and Klebsiella pneumonia. Diameter zones of inhibition range between 8mm to 26mm.respectively. It was observed that Minimum inhibitory concentration (MIC) of Parkia biglobosa extracts against the multiple resistant Uropathogenic bacteria ranges between 6.25mg/ml and 12.5mg/ml for Stem bark and leaf extracts respectively. Pseudomonas aeruginosa has a higher value of Minimum inhibitory concentration (MIC) of Parkia biglobosa of 12.5mg/ml for Stem bark and 25mg/ml for leaf extracts.The major phytochemical constituent of interests such as Polyphenol, Cardiac glycosides, Alkaloids, Tannins Saponin and Flavonoids were found to be present in Parkia biglobosa. In comparing the quantitative content of phytochemical of Parkia biglobosa.Stem bark extract contains large quantity of phytochemical compare to the leaf extract. These constituents are also higher in acetone extract than ethanol extract.These results justifies the uses of Parkia biglobosa in traditional medicine and pave the way for further studies in the developing a new drug.

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Section: Discussionsupporting

confidence: 92%

Section: Estimation Of Total Flavonoid Concentrationmentioning

confidence: 99%

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Comparative study between the effect of Parkia Biglobosa (JACQ) benth and conventional antibiotics against multiple antibiotic resistant uropathogenic bacteria (MARUB)

Osuntokun¹,

Jemilaiye²,

Ar³

2018

MOJBB

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“…In this study, E. coli was the most common bacteria isolated from urine of pregnant women with UTI followed by K. pneumoniae and A. baumannii as shown in Table 1. Gram-negative bacteria belong to enterobacteriaceae family is one of the most etiological agents of UTI with percentage in-between 80% to 90% [21,22]. While, grampositive bacteria comes in the second degree, for example, S. saprophyticus is the second most common cause UTI in females with percentage between 30% to 40% [23].…”

Section: Discussionmentioning

confidence: 99%

In vitro antibacterial activity of an aqueous extracts of Matricaria chmomilla flowers against pathogenic bacteria isolated from pregnant women with urinary tract infection

Aljanaby¹

2018

biomedicalresearch

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“…Other investigators were performed of the VITEK 2 system for identification and susceptibility testing of Enterobacteriaceae clinical isolates, [7]. Scientists conducted a numerous techniques (PCR, gel electrophoresis, 16S ribosomal RNA gene sequencing and phylogenetic analysis) to identify the diversity of Enterobacteriaceae species, [8,9]. Small rRNA gene sequencing, particularly 16SrRNA sequencing in bacteria, have led to advances on multiple fronts in microbiology.…”

Section: Introductionmentioning

confidence: 99%

Molecular Study of 16SrRNAGene in Enterobacteriaceaeisolated from Iraqi Patients

Mahmood¹,

Turkey²,

Khalaf³

2018

ANJS

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This study included the collection of 204 clinical and non-clinical samples from Ibn-Al-Baladi childbirth Hospital, Al-Yarmouk Teaching Hospital and Imam Ali Hospital in Baghdad, from both genders of different ages. The collected samples were distributed according to the collection source (urine, wounds, burns, feces, Tigris River water in Baghdad and soil samples). A total of isolates of Escherichia coli (48.43 %), 15 isolates Klebsiella pneumoniae (23.43 %), 10 isolates of Enterobacter cloacae (15.62 %) and 8 isolates of proteus mirabilis (12.50 %) were isolated and identified based on microscopic culture, conventional methods, VITEK 2 and molecular identification of 16 SrRNA gene. Our investigations indicated that Escherichia coli and Klebsiella pneumoniae species represent the most frequent isolates, whereas both Enterobacter cloacae and Proteus mirabilis were the less frequent species. The results of the analysis of the sequencing of selected 21 isolates were deposited in the National Center for Biotechnology Information (NCBI) belong to the four species (From

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Molecular identification of multi drug resistant bacteria from urinary tract infected urine samples

Cited by 32 publications

References 42 publications

Comparative study between the effect of Parkia Biglobosa (JACQ) benth and conventional antibiotics against multiple antibiotic resistant uropathogenic bacteria (MARUB)

Comparative study between the effect of Parkia Biglobosa (JACQ) benth and conventional antibiotics against multiple antibiotic resistant uropathogenic bacteria (MARUB)

In vitro antibacterial activity of an aqueous extracts of Matricaria chmomilla flowers against pathogenic bacteria isolated from pregnant women with urinary tract infection

Molecular Study of 16SrRNAGene in Enterobacteriaceaeisolated from Iraqi Patients

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